For speech enhancement or blind signal extraction (BSE), estimating interference and noise characteristics is decisive for its performance. For multichannel approaches using multiple microphone signals, a BSE scheme combining a blocking matrix (BM) and spectral enhancement filters was proposed in numerous publications. For such schemes, the BM provides a noise estimate by suppressing the target signal only. The estimated noise reference is then used to design spectral enhancement filters for the purpose of noise reduction. For designing the BM, 'Directional Blind Source Separation (BSS)' was already proposed earlier. This method combines a generic BSS algorithm with a geometric constraint derived from prior information on the target source position to obtain an estimate for all interfering point sources and diffuse background noise. In this paper, we provide a theoretical analysis to show that Directional BSS converges to a relative transfer function (RTF)-based BM. The behavior of this informed signal separation scheme is analyzed and the blocking performance of Directional BSS under various acoustical conditions is evaluated. The robustness of Directional BSS regarding the localization error for the target source position is verified by experiments. Finally, a BSE scheme combining Directional BSS and Wiener-type spectral enhancement filters is described and evaluated.