Multidrug resistance and integron carriage in clinical isolates ofPseudomonas aeruginosa in Tehran, Iran

Abstract

Background/aim: Pseudomonas aeruginosa is the cause of 10% of hospital-acquired infections. The organisms are often multidrugresistant, mediated mostly by antibiotic-resistant integrons. The aim of this research was to study integron carriage and its association with multidrug resistance in burn and nonburn clinical isolates of P. aeruginosa.Materials and methods: A total of 112 P. aeruginosa clinical isolates were collected from the Motahari and Shohadaye Tajrish hospitals in Tehran between July and December 2011. Antibiotic susceptibility to 13 antibiotics was determined by disk diffusion. Detection of integron classes 1 and 2 and amplifications of internal variable regions (IVRs) of class 1 integrons were carried out by PCR and specific primers.Results: Among the 112 isolates, 77 were from burn patients and 35 were nonburn isolates. Multidrug resistance and class 1 integron carriage were both significantly higher in the burn isolates compared to the nonburn strains (97.4% vs. 22.8% and 82.3% vs. 17.7%, respectively). Class 2 integron (2.7%) was only present in the burn isolates. Amplification of IVRs of class 1 integrons revealed 3 different fragment arrays. Conclusion:The significant association between multidrug resistance and integron carriage among P. aeruginosa burn isolates suggests a dissemination of resistance determinants by horizontal gene transfer.

Antibacterial Activity of Chitosan and PAβN on MexAB Expression in Clinical Isolates of Ciprofloxacin Resistant Pseudomonas aeruginosa

“…Following the disk diffusion method, the highest resistance rate of 70% was observed in meropenem and piperacillin/tazobactam, while the lowest resistance rate of 40% was seen in tobramycin. In a study by Goudarzi in Iran, the frequency of MDR strains was 74.1%, which is close to our results of 76.7% (10). In a study by Pokhare et al, of all isolates, 28.2% were resistant to tobramycin, 23.9% to piperacillin/tazobactam, and 34.7% to meropenem (17).…”

<p>Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of <em>Pseudomonas aeruginosa</em></p>

“…In contrast, Shahcheraghi et al in Tehran reported 5.4% of their isolates were MDR. 14 Other studies in Iran 1,2,4,15,16 showed different rates (40% to 97.5%) of MDR clinical isolates of P. aeruginosa based on different sources of samples and hospital wards of their collection. However, we found that P. aeruginosa isolated from the catheters and burn wounds are more capable for the acquisition of antibiotic resistance genes, as exhibited the highest prevalence of MDR phenotypes (Table 4).…”

Multidrug resistant Pseudomonas aeruginosa in Iran: A systematic review and metaanalysis

“…Finally, 350 articles were retained for detailed full-text evaluation. According to quality assessment criteria and inclusion/exclusion criteria, a total of 23 articles with full text reporting the prevalence of MDR P. aeruginosa were recruited for the systematic review and meta-analysis[1112131415161718192021222324252627282930313233] [Table 1]. In total, 10 studies from Tehran, 3 studies from Isfahan, 3 studies from Ahvaz, 2 studies from Orumieh, 1 study from Zahedan, 1 study from Zanjan, 1 study from Tabriz, 1 study from Guilan, and 1 study from Hamedan were involved.…”

“…In total, 10 studies from Tehran, 3 studies from Isfahan, 3 studies from Ahvaz, 2 studies from Orumieh, 1 study from Zahedan, 1 study from Zanjan, 1 study from Tabriz, 1 study from Guilan, and 1 study from Hamedan were involved. [1112131415161718192021222324252627282930313233] Figure 2 shows the distribution of MDR P. aeruginosa in different parts of Iran. By using random-effect models, the pooled prevalence of MDR P. aeruginosa was estimated to be 58% (95% confidence interval [CI]; 0.54–0.61).…”