Multidrug resistance dissemination by extended-spectrum β-lactamase-producing Escherichia coli causing community-acquired urinary tract infection in the Central-Western Region, Brazil

Gonçalves, Laura Fernandes; Martins-Júnior, Paulo de Oliveira; Melo, Ana Beatriz Fabrício de; Silva, Rafaella Christina Rocha Moreira da; Martins, Vívian T.; Pitondo-Silva, André; Campos, Tatiana Amabile de

doi:10.1016/j.jgar.2016.02.003

Cited by 42 publications

(33 citation statements)

References 21 publications

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“…The rate of ESBL producing E. coli isolates in Nepal, Paris, Karachi, central India and Shiraz were 91/7%, 69/4%, 54%, 41/6% and 69/2%, respectively, which are high as compared to our results. In contrast, according to a Brazilian report of 2013, the figure was 7/1% which is lower than the results of our study [18,21,[27][28][29][30]. Contrary to our study, in Isfahan in 2012, E. coli isolates showed lower resistance against ceftazidime (32%), cefotaxime (38%) and ciprofloxacin (33/4%), while in Shiraz, resistant to ciprofloxacin and ceftazidim was 38/5% and 63/5%, respectively [20,29].…”

Section: Discussioncontrasting

confidence: 99%

Prevalence and Antibiotic Susceptibility Pattern of Extended Spectrum Beta Lactamases Producing Escherichia Coli Isolated from Outpatients with Urinary Tract Infections in Babol, Northern of Iran

Baziboroun

Bayani

Poormontaseri

et al. 2018

Current Issues in Pharmacy and Medical Sciences

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Received 08 November 2017 Accepted 15 February 2018 The incidence of community-acquired UTIs due to extended-spectrum beta-lactamase (ESBL) producing E. coli isolates, has increased worldwide and is considered a great problem in the treatment of infections. The aim of this study was to determine the prevalence of ESBL producing E. coli isolates in urine samples of outpatients in Babol, North of Iran. A total of 3 699 urine samples from outpatients referred to Yahyanejad Hospital, Babol, Iran, were collected during 15 months, from March 2016 to June 2017. The samples were processed for bacterial culture using conventional methods, while antimicrobial susceptibility testing for E. coli isolates was performed by the disc diffusion method. The prevalence of ESBL producing E. coli isolates were assessed by Double-Disc tests. Of 3699 tested sample, 201 samples showed the growth of pathogens and among them, 106 isolates (52/7%) were E. coli. The rate of ESBL producing E. coli isolates was 25/4%.The majority (81/5%) of the isolates were from females. High percent resistance was found against ceftazidime, cefotaxime, ceftriaxone, cefixime and ciprofloxacin, ranging from 61-100%, while the highest percent susceptibility was seen to meropenem, piperacillin- tazobactam (100%), followed by nitrofurantoin and amikacin (91%). The high prevalence of ESBL producing E. coli isolates from outpatients calls for the need to aware of this increasing resistance between uropathogens and update bacterial susceptibility data and to set up our empirical therapy accordingly.

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Section: Discussioncontrasting

confidence: 99%

Prevalence and Antibiotic Susceptibility Pattern of Extended Spectrum Beta Lactamases Producing Escherichia Coli Isolated from Outpatients with Urinary Tract Infections in Babol, Northern of Iran

Baziboroun

Bayani

Poormontaseri

et al. 2018

Current Issues in Pharmacy and Medical Sciences

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“…Our phylogenetic analysis evidenced that UPEC 252 belonged to phylogroup A, ST10, and serotype O71:H40. E. coli strains of the ST10 have been frequently associated with UTI in several countries, including Brazil (Gonçalves et al, 2016 ; Hertz et al, 2016 ; Campos et al, 2018 ). Other studies have also reported the isolation of E. coli strains of the ST10 from the environment and food, as well as from bloodstream infections and diarrhea, evidencing the versatility of colonization of this ST (Arais et al, 2018 ; Liu et al, 2018 ; Mohsin et al, 2018 ; Yamaji et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and Uropathogenic Escherichia coli (aEPEC/UPEC) Strain

Valiatti

Santos

et al. 2020

Front. Cell. Infect. Microbiol.

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Hybrid strains of Escherichia coli combine virulence traits of diarrheagenic (DEC) and extraintestinal pathogenic E. coli (ExPEC), but it is poorly understood whether these combined features improve the virulence potential of such strains. We have previously identified a uropathogenic E. coli (UPEC) strain (UPEC 252) harboring the eae gene that encodes the adhesin intimin and is located in the locus of enterocyte effacement (LEE) pathogenicity island. The LEE-encoded proteins allow enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) to form attaching and effacing (A/E) lesions in enterocytes. We sought to characterize UPEC 252 through whole-genome sequencing and phenotypic virulence assays. Genome analysis unveiled that this strain harbors a complete LEE region, with more than 97% of identity comparing to E2348/69 (EPEC) and O157:H7 Sakai (EHEC) prototype strains, which was functional, since UPEC 252 expressed the LEE-encoded proteins EspB and intimin and induced actin accumulation foci in HeLa cells. Phylogenetic analysis performed comparing 1,000 single-copy shared genes clustered UPEC 252 with atypical EPEC strains that belong to the sequence type 10, phylogroup A. Additionally, UPEC 252 was resistant to the bactericidal power of human serum and colonized cells of the urinary (T24 and HEK293-T) and intestinal (Caco-2 and LS174T) tracts. Our findings suggest that UPEC 252 is an atypical EPEC strain that emerges as a hybrid strain (aEPEC/UPEC), which could colonize new niches and potentially cause intestinal and extraintestinal infections.

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“…Recent studies have reported that ESBL-producing Enterobacteriaceae are now detected in a significant rate in outpatients presenting cystitis. In a public institution located in Brasilia, the ESBL production rate in E. coli collected from July 2013 to April 2014 was 7.1% 13 . When we reviewed all publication from Brazil about ESBLs, CTX-M-2 was the most frequently detected enzyme and was also detected in the largest number of different Enterobacteriaceae species (Table 1).…”

Section: The Rise Of Extended-spectrum β-Lactamasesmentioning

confidence: 99%

Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins

Sampaio

Gales

2016

Brazilian Journal of Microbiology

111

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During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96.2%) in carbapenem resistant K. pneumoniae. To date KPC-2 is the only variant reported in Brazil. Polymyxin B resistance in KPC-2-producing K. pneumoniae has come to an alarming rate of 27.1% in 2015 in São Paulo, the largest city in Brazil. New Delhi metallo-β-lactamase was detected in Brazil in 2013, has been reported in different Brazilian states but are not widely disseminated. Antimicrobial resistance in Enterobacteriaceae in Brazil is a very serious problem that needs urgent actions which includes both more strict adherence to infection control measures and more judicious use of antimicrobials.

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Multidrug resistance dissemination by extended-spectrum β-lactamase-producing Escherichia coli causing community-acquired urinary tract infection in the Central-Western Region, Brazil

Cited by 42 publications

References 21 publications

Prevalence and Antibiotic Susceptibility Pattern of Extended Spectrum Beta Lactamases Producing Escherichia Coli Isolated from Outpatients with Urinary Tract Infections in Babol, Northern of Iran

Prevalence and Antibiotic Susceptibility Pattern of Extended Spectrum Beta Lactamases Producing Escherichia Coli Isolated from Outpatients with Urinary Tract Infections in Babol, Northern of Iran

Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and Uropathogenic Escherichia coli (aEPEC/UPEC) Strain

Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins

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