Multidrug resistance protein gene expression in<i><scp>T</scp></i><i>richoplusia ni</i>caterpillars

Simmons, James G.; D'Souza, Olivia; Rheault, Mark R.; Donly, Cam

doi:10.1111/imb.12003

Cited by 29 publications

(21 citation statements)

References 51 publications

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“…PxPgp1 expressions in the head and integument were equally low. This is consistent with the other lepidopteran, the cabbage looper, T. ni , in which Pgp was most abundant in the midgut and followed by the malpighian tubules [54]. Pgp was also highly expressed in the malpighian tubules of Manduca sexta larvae [55].…”

Section: Resultssupporting

confidence: 87%

Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

Liu

Yang

Hou

et al. 2013

IJMS

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Macrocyclic lactones such as abamectin and ivermectin constitute an important class of broad-spectrum insecticides. Widespread resistance to synthetic insecticides, including abamectin and ivermectin, poses a serious threat to the management of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a major pest of cruciferous plants worldwide. P-glycoprotein (Pgp), a member of the ABC transporter superfamily, plays a crucial role in the removal of amphiphilic xenobiotics, suggesting a mechanism for drug resistance in target organisms. In this study, PxPgp1, a putative Pgp gene from P. xylostella, was cloned and characterized. The open reading frame (ORF) of PxPgp1 consists of 3774 nucleotides, which encodes a 1257-amino acid peptide. The deduced PxPgp1 protein possesses structural characteristics of a typical Pgp, and clusters within the insect ABCB1. PxPgp1 was expressed throughout all developmental stages, and showed the highest expression level in adult males. PxPgp1 was highly expressed in midgut, malpighian tubules and testes. Elevated expression of PxPgp1 was observed in P. xylostella strains after they were exposed to the abamectin treatment. In addition, the constitutive expressions of PxPgp1 were significantly higher in laboratory-selected and field-collected resistant strains in comparison to their susceptible counterpart.

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Section: Resultssupporting

confidence: 87%

Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

Liu

Yang

Hou

et al. 2013

IJMS

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“…40 Multidrug resistance-associated protein 2 (MRA) is an ATP-dependent transporter expressed at the brush border membrane of the enterocyte that confers protection against absorption of toxicants from foods or bile, localization of MRP2 in the intestine may be subject to dynamic equilibrium between the plasma membrane and intracellular domains, thus allowing for rapid regulation of MRP2 function. 41,42 Our results also implied that MRP2 may have a crucial role in the detoxification of imidacloprid in S. graminum. Leptin reduced PPAR 2 expression at the promoter, mRNA and protein levels in hepatic stellate cells, which required -catenin, mitogen-activated protein kinase and delta-like homolog 1 (DLK1) signaling pathways.…”

Section: Discussionsupporting

confidence: 58%

Differential expression of genes in greenbug (Schizaphis graminum Rondani) treated by imidacloprid and RNA interference

Liu

et al. 2019

Pest Management Science

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BACKGROUND Insecticides act as toxins, inhibitors of digestion and deterrents, and affect the expression of many genes in insects. To assess key genes associated with the detoxification or regulation of imidacloprid in greenbug, Schizaphis graminum (Rondani), the transcriptome and digital gene expression (DGE) profile were analyzed using Illumina sequencing. RESULTS In total, 48 763 494 clean reads were obtained by sequencing. Expression profile analysis showed that 2782 unigenes were differently expressed between the imidacloprid treatment and control groups. After exposure to imidacloprid, the expression levels of 1846 unigenes were upregulated and 936 were downregulated in comparison with controls. Expression patterns of the top 20 highly expressed genes show that they could be involved in the detoxification of imidacloprid. Silencing of multidrug resistance‐associated gene (MRA), GATA‐binding gene (GAT) and takeout‐like precursor gene (TLP) resulted in increasing susceptibility to imidacloprid. CONCLUSIONS The differentially expressed genes in S. graminum have potential regulatory or detoxification roles in response to imidacloprid. These results should be useful in understanding the molecular mechanisms of greenbug adaption to imidacloprid. © 2018 Society of Chemical Industry

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“…Enhanced P‐gp gene expression was also previously observed in rodent tissues and cell cultures exposed to organochlorine and organophosphate pesticides (Shabbir et al ., ; Lecoeur et al ., ). In insects, increased MDR 2 expression has been observed in the midgut and Malpighian tubules of Trichoplusia ni caterpillars after oral exposure to deltamethrin (Simmons et al ., ). Different exposure methods to ivermectin also resulted in the over‐expression of ABC transporter genes in human body lice (Yoon et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Silencing ofP‐glycoproteinincreases mortality in temephos‐treatedAedes aegyptilarvae

Figueira-Mansur

Ferreira‐Pereira

Mansur

et al. 2013

Insect Molecular Biology

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Re-emergence of vector-borne diseases such as dengue and yellow fever, which are both transmitted by the Aedes aegypti mosquito, has been correlated with insecticide resistance. P-glycoproteins (P-gps) are ATP-dependent efflux pumps that are involved in the transport of substrates across membranes. Some of these proteins have been implicated in multidrug resistance (MDR). In this study, we identified a putative P-glycoprotein in the Ae. aegypti database based on its significantly high identity with Anopheles gambiae, Culex quinquefasciatus, Drosophila melanogaster and human P-gps. The basal ATPase activity of ATP-binding cassette transporters in larvae was significantly increased in the presence of MDR modulators (verapamil and quinidine). An eightfold increase in Ae. aegypti P-gp (AaegP-gp) gene expression was detected in temephos-treated larvae as determined by quantitative PCR. To analyse the potential role of AaegP-gp in insecticide efflux, a temephos larvicide assay was performed in the presence of verapamil. The results showed an increase of 24% in temephos toxicity, which is in agreement with the efflux reversing effect. RNA interference (RNAi)-mediated silencing of the AaegP-gp gene caused a significant increase in temephos toxicity (57%). In conclusion, we have demonstrated for the first time in insects that insecticide-induced P-gp expression can be involved in the modulation of insecticide efflux.

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Multidrug resistance protein gene expression inTrichoplusia nicaterpillars

Cited by 29 publications

References 51 publications

Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

Differential expression of genes in greenbug (Schizaphis graminum Rondani) treated by imidacloprid and RNA interference

Silencing ofP‐glycoproteinincreases mortality in temephos‐treatedAedes aegyptilarvae

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