Multidrug-resistant Enterobacteriaceae from indoor air of an urban wastewater treatment plant

Teixeira, Juliana V.; Cecílio, Pedro; Gonçalves, Daniela; Vilar, Vítor J.P.; Pinto, Eugénia; Ferreira, Helena

doi:10.1007/s10661-016-5382-4

Cited by 24 publications

(7 citation statements)

References 22 publications

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“…However, the diversity in the US air samples was overall lower than in Beijing smog. Using culture-based approaches, air samples from wastewater treatment plants, animal slaughter houses and swine feeding operations have earlier been shown to carry resistant bacteria [ 38 – 40 ]. In addition, aerial transport of antibiotic-resistant bacteria from cattle feed yards was recently proposed [ 41 ], but in general, the primary sources and the importance of resistant bacteria in urban air are not clear.…”

Section: Discussionmentioning

confidence: 99%

The structure and diversity of human, animal and environmental resistomes

et al. 2016

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BackgroundAntibiotic resistance genes (ARGs) are widespread but cause problems only when present in pathogens. Environments where selection and transmission of antibiotic resistance frequently take place are likely to be characterized by high abundance and diversity of horizontally transferable ARGs. Large-scale quantitative data on ARGs is, however, lacking for most types of environments, including humans and animals, as is data on resistance genes to potential co-selective agents, such as biocides and metals. This paucity prevents efficient identification of risk environments.ResultsWe provide a comprehensive characterization of resistance genes, mobile genetic elements (MGEs) and bacterial taxonomic compositions for 864 metagenomes from humans (n = 350), animals (n = 145) and external environments (n = 369), all deeply sequenced using Illumina technology. Environment types showed clear differences in both resistance profiles and bacterial community compositions. Human and animal microbial communities were characterized by limited taxonomic diversity and low abundance and diversity of biocide/metal resistance genes and MGEs but a relatively high abundance of ARGs. In contrast, external environments showed consistently high taxonomic diversity which in turn was linked to high diversity of both biocide/metal resistance genes and MGEs. Water, sediment and soil generally carried low relative abundance and few varieties of known ARGs, whereas wastewater/sludge was on par with the human gut. The environments with the largest relative abundance and/or diversity of ARGs, including genes encoding resistance to last resort antibiotics, were those subjected to industrial antibiotic pollution and a limited set of deeply sequenced air samples from a Beijing smog event.ConclusionsOur study identifies air and antibiotic-polluted environments as under-investigated transmission routes and reservoirs for antibiotic resistance. The high taxonomic and genetic diversity of external environments supports the hypothesis that these also form vast sources of unknown resistance genes, with potential to be transferred to pathogens in the future.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-016-0199-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

The structure and diversity of human, animal and environmental resistomes

et al. 2016

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“…It is worth noticing that compare to GNB, GPB dominant much more in ambient air, which we believe may influence this difference of MDR occupation percentage to a certain extent. Still, the situation of MDR bacteria occupation in the ambient air was serious, approaching the percentage of airborne MDR bacteria that have been isolated from swine feeding operations (Chapin et al, 2005) or wastewater treatment plants (Teixeira et al, 2016). MDR bacteria occupied approximately one forth in both polluted or non-polluted weather, which also suggest the serious situation of airborne MDR bacteria.…”

Section: Discussionmentioning

confidence: 99%

Comparison of culturable antibiotic-resistant bacteria in polluted and non-polluted air in Beijing, China

Mao

Ding

Wang

et al. 2019

Environment International

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Background: Air pollution has been a serious health issue in Beijing for years. Airborne antibiotic-resistant bacteria could be a potential health crisis as reserve of antibiotic resistance transmission in environment. The composition and antibiotic resistance pattern of culturable bacterial community and how these are affected by air pollution remain unclear.Objectives: This study aimed to compare the compositions and antibiotic resistance patterns of culturable bacteria in polluted and non-polluted weather conditions in Beijing. Methods: Air samples were collected indoors and outdoors during polluted and non-polluted weather using sixstage Andersen Samplers. For each isolated bacterium, the 16S ribosomal RNA gene was amplified, sequenced, and blasted against the National Center for Biotechnology Information database Antibiotic resistance was conducted by antimicrobial susceptibility testing. Results: Bacterial concentration in polluted weather was significantly higher than in non-polluted weather, both indoors and outdoors (P < 0.05). Gram-positive bacteria (GPB) were dominant in both weathers but gramnegative bacteria (GNB) were more abundant in polluted weather than non-polluted weather both indoors and outdoors. Multidrug-resistant (MDR) bacteria occupied 23.7% of all bacterial isolates, 22.4% of isolates from polluted weather and 27.8% of isolates from non-polluted weather. Penicillins were resisted by 72.4% and 83.3% of isolates from polluted and non-polluted weather, respectively. Conclusions: The bacterial concentration was significantly higher in polluted weather, compared to non-polluted weather. Polluted weather is correlated with changes in the bacterial composition in the air, with a greater abundance of GNB. Penicillins was resisted by over 70% of bacterial isolates. The abundance of MDR bacteria suggested potential risks for human health.

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“…The supernatant was then collected and stored at 4°C until further use. Relevant beta-lactamase [ bla TEM , bla OXA, bla SHV ( Dallenne et al, 2010 ) , and bla CTX-M group 1 ( Machado et al, 2005 )] and carbapenemase [ bla VIM , bla IMP , bla KPC , bla OXA-48 , and bla NDM ( Poirel et al, 2011 )] genes were screened using the primers and amplification conditions described in the literature ( Machado et al, 2005 ; Dallenne et al, 2010 ; Poirel et al, 2011 ; Goncalves et al, 2016 ; Teixeira et al, 2016 ). Whenever relevant, amplicons were sequenced using the ABI-PRISM 3100 automatic genetic analyzer (Thermo Fischer Scientific, Waltham, MA, United States).…”

Section: Methodsmentioning

confidence: 99%

Intra- and Extra-Hospital Dissemination of IMP-22-Producing Klebsiella pneumoniae in Northern Portugal: The Breach of the Hospital Frontier Toward the Community

et al. 2021

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The emergence of infections (and colonization) with Enterobacteriaceae-producing carbapenemases is a threatening public health problem. In the last decades, we watched an isolated case becoming a brutal outbreak, a sporadic description becoming an endemic problem. The present study aims to highlight the dissemination of IMP-22-producing Klebsiella pneumoniae in the North of Portugal, through the phenotypic and genotypic characterization of isolates collected from hospitalized patients (n=5) and out-patients of the emergency ward of the same acute care hospital (n=2), and isolates responsible for the intestinal colonization of residents in a Long-Term Care Facility (n=4). Pulsed-field gel electrophoresis (PFGE) results, associated with conjugation experiments pointed to a pattern of both vertical and horizontal dissemination. Overall, and complementing other studies that give relevance to IMP-22-producing K. pneumoniae in the clinical settings, here we show for the first time the public health threatening breach of the hospital frontier of this resistance threat, toward the community.

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Multidrug-resistant Enterobacteriaceae from indoor air of an urban wastewater treatment plant

Cited by 24 publications

References 22 publications

The structure and diversity of human, animal and environmental resistomes

The structure and diversity of human, animal and environmental resistomes

Comparison of culturable antibiotic-resistant bacteria in polluted and non-polluted air in Beijing, China

Intra- and Extra-Hospital Dissemination of IMP-22-Producing Klebsiella pneumoniae in Northern Portugal: The Breach of the Hospital Frontier Toward the Community

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