Multidrug-resistant Salmonella strains from food animals as a potential source for human infection in Iran

Manzari, Mojgan; Fani, Fereshteh; Alebouyeh, Masoud; Moaddeli, Alaleh; Farzami, Marjan Rahnamaye; Shahidi, Maneli Amin; Shekarforoush, Seyed Shahram

doi:10.1016/j.cimid.2022.101898

Cited by 3 publications

(4 citation statements)

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“…Salmonella is one of the most widespread pathogenic foodborne bacteria in Chinese food products and retail chicken have been recognized as one of the major sources of human salmonellosis ( Paudyal et al, 2018 ). Moreover, there has been an increase in reports of the dissemination of resistant Salmonella from chicken to humans through the chicken production chain ( Kongsanan et al, 2021 ; Manzari et al, 2022 ). In this study, we surveyed the prevalence, serovar distribution, MDR, and genomic characteristics of Salmonella in chilled chicken carcass and humans with diarrhea sources in Qingdao, China in 2020.…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of Salmonella isolated from retail chicken and humans with diarrhea in Qingdao, China

Wang,

Cui,

Liu

et al. 2023

Front. Microbiol.

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Salmonella, especially antimicrobial resistant strains, remains one of the leading causes of foodborne bacterial disease. Retail chicken is a major source of human salmonellosis. Here, we investigated the prevalence, antimicrobial resistance (AMR), and genomic characteristics of Salmonella in 88 out of 360 (24.4%) chilled chicken carcasses, together with 86 Salmonella from humans with diarrhea in Qingdao, China in 2020. The most common serotypes were Enteritidis and Typhimurium (including the serotype I 4,[5],12:i:-) among Salmonella from both chicken and humans. The sequence types were consistent with serotypes, with ST11, ST34 and ST19 the most dominantly identified. Resistance to nalidixic acid, ampicillin, tetracycline and chloramphenicol were the top four detected in Salmonella from both chicken and human sources. High multi-drug resistance (MDR) and resistance to third-generation cephalosporins resistance were found in Salmonella from chicken (53.4%) and humans (75.6%). In total, 149 of 174 (85.6%) Salmonella isolates could be categorized into 60 known SNP clusters, with 8 SNP clusters detected in both sources. Furthermore, high prevalence of plasmid replicons and prophages were observed among the studied isolates. A total of 79 antimicrobial resistant genes (ARGs) were found, with aac(6′)-Iaa, blaTEM-1B, tet(A), aph(6)-Id, aph(3″)-Ib, sul2, floR and qnrS1 being the dominant ARGs. Moreover, nine CTX-M-type ESBL genes and the genes blaNMD-1, mcr-1.1, and mcr-9.1 were detected. The high incidence of MDR Salmonella, especially possessing lots of mobile genetic elements (MGEs) in this study posed a severe risk to food safety and public health, highlighting the importance of improving food hygiene measures to reduce the contamination and transmission of this bacterium. Overall, it is essential to continue monitoring the Salmonella serotypes, implement the necessary prevention and strategic control plans, and conduct an epidemiological surveillance system based on whole-genome sequencing.

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Section: Discussionmentioning

confidence: 99%

Genomic characterization of Salmonella isolated from retail chicken and humans with diarrhea in Qingdao, China

Wang,

Cui,

Liu

et al. 2023

Front. Microbiol.

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“…Between 2013 and 2019 in Iran, Salmonella caused 350 (16.7%) of the 2098 reported foodborne outbreaks (Manzari et al., 2022 ). Several Iranian studies have examined the prevalence and characteristics of Salmonella .…”

Section: Introductionmentioning

confidence: 99%

“…Of the 105 Salmonella isolates obtained, most (70%) originated from chicken meat, followed by beef (19%), lamb (11%) and human stool (1.7%). The predominant serotypes detected were Salmonella infantis (40.9%), S. enteritidis (29.5%) and Salmonella paratyphi B (8.6%) (Manzari et al., 2022 ). In a study across 6 provinces in Iran (Tehran, Qazvin, Mazandaran, West‐Azerbaijan, Khuzestan and Sistan‐Baluchestan), a total of 3125 samples were examined for Salmonella contamination, including 2250 poultry samples and 875 poultry egg samples.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and antimicrobial susceptibility of Salmonella enteritidis and Salmonella typhimurium isolated from hen eggs and quail eggs in Karaj, Iran

Yousefi Amin,

Oshaghi,

Habibi

et al. 2024

Veterinary Medicine & Sci

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Background and aimDifferent Salmonella serotypes are considered one of the most important food pathogens in the world. Poultry meat and eggs are the primary carriers of Salmonella in human populations. This study aimed to estimate the Salmonella enteritidis and Salmonella typhimurium contamination rates of retail hen and quail eggs in Karaj, Iran. Moreover, the antimicrobial resistance patterns of the strains were evaluated, and the efficiency of the standard culture method and multiplex polymerase chain reaction (m‐PCR) were compared.Materials and methodsIn this descriptive cross‐sectional study over 1 year (Jan–Dec 2022), 150 commercial and 150 backyard hen eggs and 300 commercial quail eggs, without cracks and fractures, were collected randomly from best selling groceries in Karaj city. All samples were examined for Salmonella contamination independently by standard culture and m‐PCR approaches. A standard disc diffusion method was employed to assess the antimicrobial susceptibility of the strains against 18 antimicrobial agents.ResultsOut of 300 examined eggs, 2 S. enteritidis strains were isolated from the shell of backyard hen eggs. The same serotype was also detected in the contents of one of these two eggs. One S. typhimurium was isolated from the shell of a commercial hen egg. Overall, the Salmonella contamination of the shell and contents was 1% and 0.3%, respectively. Salmonella was not isolated from the eggshells or the contents of the quail eggs. There was complete agreement between the results of m‐PCR and the standard culture methods. Among the 18 tested antibiotics, the highest resistance was recorded for colistin (100%), followed by nalidixic acid (75%).ConclusionAs most Salmonella spp. are associated with human food poisoning, continuous surveillance is required to effectively reduce the risk posed by contaminated poultry eggs. Furthermore, mandatory monitoring of antimicrobial use on Iranian poultry farms is recommended.

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Characterization of Virulence Genotypes, Antimicrobial Resistance Patterns, and Biofilm Synthesis in Salmonella spp Isolated from Foodborne Outbreaks

Soltan Dallal,

Nasser,

Karimaei

2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Salmonella is the main bacterial pathogen that causes foodborne disease, particularly in developing countries. Nontyphoidal Salmonella (NTS) include Enteritidis and Typhimurium as the most prevalent strains which are one of the significant causes of acute gastroenteritis in children. Therefore, identifying the most predominant serovars, types of common contaminated food, and paying attention to their antibiotic resistance are the main factors in the prevention and control strategy of salmonellosis. This study was undertaken to evaluate the prevalence rate of serovars, the biofilm formation, antimicrobial resistance (AMR) status, and phenotypic virulence factors of Salmonella strains isolated from diarrhea samples in some cities of Iran. A total of 40 (10.41%) Salmonella isolates were recovered from 384 diarrhea samples processed and the most common serovar was Salmonella serovar Typhimurium (82.5). Also, all isolates belonging to serovar Typhimurium showed more virulence factors compared to other serovars. The isolates showed a high resistance rate to ampicillin (95%) and nalidixic acid (87.5%), while a low resistance rate was found for chloramphenicol (2.5%). Moreover, significant variances in the capacity of biofilm formation were found between different Salmonella serotypes. The resistance of NTS to extant choice drugs is a potential public health problem. Constant monitoring of AMR pattern and virulence profile of NTS serovars is suggested for the prevention of salmonellosis in humans.

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Multidrug-resistant Salmonella strains from food animals as a potential source for human infection in Iran

Cited by 3 publications

References 42 publications

Genomic characterization of Salmonella isolated from retail chicken and humans with diarrhea in Qingdao, China

Genomic characterization of Salmonella isolated from retail chicken and humans with diarrhea in Qingdao, China

Prevalence and antimicrobial susceptibility of Salmonella enteritidis and Salmonella typhimurium isolated from hen eggs and quail eggs in Karaj, Iran

Characterization of Virulence Genotypes, Antimicrobial Resistance Patterns, and Biofilm Synthesis in Salmonella spp Isolated from Foodborne Outbreaks

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