Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Tada, Tatsuya; Nhung, Pham Hong; Miyoshi‐Akiyama, Tohru; Shimada, Kayo; Tsuchiya, Mitsuhiro; Phuong, Doan Mai; Anh, Nguyen Quoc; Ohmagari, Norio; Kirikae, Teruo

doi:10.1128/aac.01177-16

Cited by 28 publications

(44 citation statements)

References 38 publications

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“…The selected isolates, consisting of Gram-negative bacteria producing a variety of carbapenemases, were readily available in the two participating laboratories (10)(11)(12)(13)(14)(15)(16)(17). These isolates included 23 GES-5producing isolates obtained throughout Japan and not epidemiologically related; GES-5 producers were included because they have spread and are causing serious problems in medical settings throughout Japan (unpublished data).…”

Section: Methodsmentioning

confidence: 99%

A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species

et al. 2017

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The carbapenem inactivation method (CIM) and modified CIM (mCIM) are simple and economical phenotypic screening methods for detecting carbapenemase production in Gram-negative bacteria. Although the mCIM has been recommended by the Clinical and Laboratory Standards Institute, both the CIM and mCIM have limitations. This study describes another modified CIM, called CIMTris, in which carbapenemase was extracted from bacteria with 0.5 M Tris-HCl (pH 7.6) buffer. The ability of the CIMTris to detect carbapenemase production was examined in and species. The CIMTris had an overall sensitivity of 97.6% and an overall specificity of 92.6%, whereas the mCIM had a sensitivity of 45.1% and a specificity of 100% for the isolates tested. These findings indicate that the CIMTris is useful for detecting carbapenemase production in and species.

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Section: Methodsmentioning

confidence: 99%

A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species

et al. 2017

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“…The presence of tellurium, mercury, and arsenic operons in pEHZJ1 was consistent with the conclusion that IncHI2 plasmids usually harbor toxin-antitoxin systems and heavy metal resistance from, Aoki et al 40 These metal-resistant operons may make the strains carrying IncHI2type plasmid to be more viable in diverse environments. Thus far, two reports have studied the genetic features surrounding bla IMP-26 in P. aeruginosa, 17,18 and one in Enterobacter cloacae. 20 Among them, the genetic context surrounding bla IMP-26 in P. aeruginosa was intI1-bla IMP-26 -qacG-aacA4-aac (6ʹ)-orf-catB3 and intI1-bla IMP-26 -qacG-aac(6ʹ)-Ib-aac(6ʹ)-orf3-orf4-catB3-dfrA1-tnpA-istB-orf5, respectively.…”

Section: Dovepressmentioning

confidence: 99%

“…Previous reports showed that bla IMP-26 has high genetic similarity to bla IMP-4 , with only one position substitution at 145 (G-to-T change), 13 moreover, bla IMP-26 and bla IMP-4 were generally carried by a class 1 integron. 17,18,46 So, we selected pIMP26, pEI1573 and our plasmid pEHZJ1 for comparative analysis of genes surrounding bla IMP-4 and bla IMP-26 . The results revealed that pIMP26 had the same genetic context surrounding bla IMP-26 as pEHZJ1, however genetic environment of bla IMP-4 in pEI1573 was intI1bla IMP-4 -qacG-aacA4-catB-qacEΔ1-sul1.…”

Section: Dovepressmentioning

confidence: 99%

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Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene blaSHV-178

Gou¹,

Liu²,

Guo³

et al. 2020

IDR

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To investigate the occurrence and genetic characteristics of the bla IMP-26-positive plasmid from a multidrug-resistant clinical isolate, Enterobacter hormaechei L51. Methods: Species identification was determined by MALDI-TOF MS and Sanger sequencing. Antimicrobial susceptibility testing was performed by the agar dilution and broth microdilution. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 and PacBio Sequel platforms, and the genome was annotated by the RAST annotation server. The ANI analysis of genomes was performed using OAT. Phylogenetic reconstruction and analyses were performed using the Harvest suite based on the core-genome SNPs of 61 publicly available E. hormaechei genomes. Results: The E. hormaechei L51 genome consists of a 5,018,729 bp circular chromosome and a 343,918 bp conjugative IncHI2/2A plasmid pEHZJ1 encoding bla IMP-26 which surrounding genetic context was intI1-bla IMP-26-ltrA-qacEΔ1-sul1. A new sequence type (ST1103) was assigned for the isolate L51 which was resistant to cephalosporins, carbapenems, but sensitive to piperacillin-tazobactam, amikacin, tigecycline, trimethoprimsulfamethoxazole and colistin. Phylogenetic analysis demonstrated that E. hormaechei L51 belonged to the same subspecies as the reference strain E. hormaechei SCEH020042, however 18,248 divergent SNP were identified. Resistance genes in pEHZJ1 including aac (3)-IIc, aac(6ʹ)-IIc, bla SHV-178 , bla DHA-1 , bla TEM-1 , bla IMP-26 , ereA2, catII, fosA5, qnrB4, tet(D), sul1 and dfrA19. Conclusion: In our study, we identified a conjugative IncHI2/2A plasmid carrying bla IMP-26 and bla SHV-178 in E. hormaechei ST1103, a novel multidrug-resistant strain isolated from China, and describe the underlying resistance mechanisms of the strain and detailed genetic context of mega plasmid pEHZJ1.

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“…Unlike the bla KPC gene, the IMP-type metallo-β-lactamase (MBL) genes, which have been reported carried by IncL/M, IncA/C, IncHI2, and IncN plasmids in Enterobacterales from Australia and China (Villa et al, 2010;Dolejska et al, 2016;Wang et al, 2017), were not frequently detected in CRKP and associated with IncFII K plasmids (Wang et al, 2018). IMP-26, which differs from IMP-4 by a single amino acid substitution (Phe49Val), was firstly reported from the Pseudomonas aeruginosa isolate in Singapore in 2010 (Koh et al, 2010;Tada et al, 2016) and was demonstrated to possess increased carbapenem-hydrolyzing activity to meropenem than IMP-1 (Tada et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate

Yao

Cheng

et al. 2020

Front. Microbiol.

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Carbapenems and tigecycline are two important classes of antimicrobial agents to treat the infections caused by Enterobacterales. Here, we reported a plasmid carrying both bla IMP−26 and tet(A) variant in clinical Klebsiella pneumoniae KP-1572. MIC results showed that K. pneumonia KP-1572 was resistant to a wide range of antimicrobials. The bla IMP−26 and tet(A) variant were located on an identical plasmid, which was indicated by S1-PFGE and southern blotting hybridization and can be successfully transferred by electroporation. Whole-plasmid sequencing and analysis revealed that a 142,993-bp-sized plasmid, designated pIMP1572, contains an IncFII k backbone and a variable region harboring bla IMP−26 and tet(A) variant. The plasmid pIMP1572 was apparently originated from a tet(A)-carrying IncFII k plasmid but with a deletion length of 6,216-bp and a multiple drug resistance region (MDRR) insertion of 25,259 bp. The plasmid pIMP1572 in the present study represents the first report of the IncFII k plasmid co-carrying bla IMP and tet(A) variant, which should be monitored.

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Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Cited by 28 publications

References 38 publications

A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species

A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species

<p>Carbapenem-Resistant <em>Enterobacter hormaechei</em> ST1103 with IMP-26 Carbapenemase and ESBL Gene <em>bla</em><sub>SHV-178</sub></p>

Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate

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