Multifaceted Neuro-Regenerative Activities of Human Dental Pulp Stem Cells for Functional Recovery after Spinal Cord Injury

Yamamoto, Akira; Matsubara, Kazuki; Saito, Kiyoshi; Kano, Fumiya

doi:10.5772/58906

Cited by 20 publications

(21 citation statements)

References 47 publications

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“…Of these, dental pulp stem cells (DPSCs) exhibit a strong proliferation and multidifferentiation ability and the advantages of noninvasive collection and easy access and culture, which are important for stem cell-based regeneration in the oral maxillofacial region, such as in dentin, periodontal and bone tissue [Khorsand et al, 2013;Woloszyk et al, 2014;Jensen et al, 2015;Tran and Doan, 2015] as well as extracranial tissues such as vascular tissue, the retina, peripheral nerves, cartilage and adipose tissue Martens et al, 2014;Mead et al, 2014;Nemeth et al, 2014;Yamamoto et al, 2014;Roozafzoon et al, 2015;Saghiri et al, 2015].…”

Section: Introductionmentioning

confidence: 99%

Demineralized Dentin Matrix Induces Odontoblastic Differentiation of Dental Pulp Stem Cells

Liu

Gao

et al. 2015

Cells Tissues Organs

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The aim of this study was to investigate the effect of demineralized dentin matrix (DDM) on dental pulp stem cells (DPSCs) and the potential of complexes with DPSCs and DDM for mineralized tissue formation. Stem cells derived from the dental pulp of healthy pigs aged 18 months were isolated and cultured. DPSCs were incubated with alpha-minimum essential medium treated with DDM extract at 1 mg/ml (DDM1) or 10 mg/ml (DDM10). The concentrations of 3 growth factors in DDM extract was measured by enzyme-linked immunosorbent assay. Adhesion of DPSCs on DDM and hydroxyapatite-tricalcium phosphate (HA-TCP) surfaces was observed using scanning electron microscopy. Cell proliferation was evaluated with cell counting kit-8 and migration by Transwell migration assays. Odontoblastic differentiation was assessed by alkaline phosphatase (ALP) and alizarin red staining, ALP activity and real-time polymerase chain reaction analysis of markers of ALP, runt-related transcription factor 2, type I collagen, dentin matrix acidic phosphoprotein-1, osteonectin and dentin sialophosphoprotein (DSPP). Finally, DPSCs were combined with DDM and placed subcutaneously in nude mice for 12 weeks; DPSCs combined with HA-TCP and DDM alone served as controls. DDM could promote DPSC adhesion, migration and odontoblastic differentiation. Mineralized tissue formation was observed with the DPSC and DDM combination and the DPSC and HA-TCP combination. The mineralized tissue of the DPSC + DDM combination stained positive for DSPP, similar to the dentin tissue. These results indicate that DDM induces DPSC odontoblastic differentiation, suggesting applications for dentin regeneration.

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Section: Introductionmentioning

confidence: 99%

Demineralized Dentin Matrix Induces Odontoblastic Differentiation of Dental Pulp Stem Cells

Liu

Gao

et al. 2015

Cells Tissues Organs

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“…More recently, healthy dental pulps are seen to be an excellent cell source for neuro-ectoderm related studies especially towards neurogenesis such as dopaminergic (DAergic)-like cells (Govindasamy et al 2010, Wang et al 2010, Kanafi et al 2013, Osathanon et al 2014, Varga & Gerber 2014, Yamamoto et al 2014. The aim of the present study was to investigate whether DPSCs from carious teeth were able to differentiate into neuronal lineage especially towards DAergic-like cells, which has yet to be reported.…”

Section: Introductionmentioning

confidence: 91%

Differentiation of stem cells derived from carious teeth into dopaminergic‐like cells

Gnanasegaran

Govindasamy²,

Kasim

2015

Int Endodontic J

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“…However, those cells make up more than 90% of DMSCs. 38 The high expression of neural markers indicates that dental stem cells can be used as an ideal seed for neural induction and regeneration. In addition to neural markers, the expression of neurotrophic factors also plays an important role.…”

Section: Usag E Of Dental S Tem Cell S In Nervous Sys Tem Impairmentmentioning

confidence: 99%

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Wang

Tian

et al. 2019

Cell Proliferation

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Nerous system diseases, both central and peripheral, bring an incredible burden onto patients and enormously reduce their quality of life. Currently, there are still no effective treatments to repair nerve lesions that do not have side effects. Stem cellbased therapies, especially those using dental stem cells, bring new hope to neural diseases. Dental stem cells, derived from the neural crest, have many characteristics that are similar to neural cells, indicating that they can be an ideal source of cells for neural regeneration and repair. This review summarizes the neural traits of all the dental cell types, including DPSCs, PDLCs, DFCs, APSCs and their potential applications in nervous system diseases. We have summed up the advantages of dental stem cells in neural repair, such as their neurotrophic and neuroprotective traits, easy harvest and low rejective reaction rate, among others. Taken together, dental stem cells are an ideal cell source for neural tissue regeneration and repair.

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Multifaceted Neuro-Regenerative Activities of Human Dental Pulp Stem Cells for Functional Recovery after Spinal Cord Injury

Cited by 20 publications

References 47 publications

Demineralized Dentin Matrix Induces Odontoblastic Differentiation of Dental Pulp Stem Cells

Demineralized Dentin Matrix Induces Odontoblastic Differentiation of Dental Pulp Stem Cells

Differentiation of stem cells derived from carious teeth into dopaminergic‐like cells

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

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