Multifunctional compartments in the nucleus: Insights from DNA and RNA localization

Clemson, Christine; Lawrence, Jeanne B.

doi:10.1002/(sici)1097-4644(199608)62:2<181::aid-jcb6>3.3.co;2-v

Cited by 13 publications

(23 citation statements)

References 28 publications

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“…The absence of speckles at the nuclear periphery ( Figure 8C) is in good correlation with the results of Brown et al (2006). We also found that other nuclear domains involved in RNA synthesis and processing and nucleoli were specifically distributed within interphase nuclei, which is in agreement with observations that Cajal bodies, PML bodies and specific RNAs lie on the surface of chromosome territories (Zirbel et al 1993, Clemson & Lawrence 1996. In our experiments, a non-random radial distribution was found not only for SC35 domains, RNAP II regions, PML bodies and nucleoli, but also for c-myc T ; therefore, this general feature of interphase nuclei could also apply for structures involved in RNA metabolism.…”

Section: Relationship Of Transcribed Genes To Nuclear Domains Involvementioning

confidence: 99%

Single-cell c-myc gene expression in relationship to nuclear domains

et al. 2008

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Nuclear locations of the c-myc gene and its transcripts (c-myc (T)) have been investigated in relation to nuclear domains involved in RNA synthesis and processing. Transcription of the c-myc gene appears to be linked to the late G(1)- and preferentially to S-phases of the cell cycle. The c-myc gene and its transcripts were positioned non-randomly within the interphase nucleus; additionally, c-myc RNA signals accumulated at nucleoli. Using oligo-probes, designed to exon II and exon III of the c-myc gene, single c-myc (T) was preferentially observed in human carcinoma HT29 and A549 cells. Conversely, human embryonal teratocarcinoma NTERA cells were characterized by the presence of multiple c-myc RNA signals located in both the nucleoli and nucleoplasm. When accumulated at nucleoli, c-myc (T) occupied the periphery of this organelle, though not those associated with the cultivation surface. In HT29 cells, approximately 80% of c-myc (T) co-localized with the RNAP II positive regions, so-called transcription factories. However, in approximately 20% of the cells with c-myc transcripts, the c-myc (T) was released from the site of synthesis, and was not associated with either transcription factories or SC35 domains. In approximately 60% of nuclei with c-myc (T), these signals were located in close proximity to the SC35 regions, but promyelocytic leukaemia bodies were associated with c-myc (T) only in approximately 20% of the nuclei. Taken together, c-myc RNA signals were positioned in the most internal parts of the cell nuclei preferentially associated with the nucleoli. Specific nuclear and nucleolar positioning probably reflects the kinetics of c-myc RNA metabolism.

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Section: Relationship Of Transcribed Genes To Nuclear Domains Involvementioning

confidence: 99%

Single-cell c-myc gene expression in relationship to nuclear domains

et al. 2008

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“…Indeed, the study by Bridger et al shows that the RNA accumulations produced from the integrated viral genes are derived from gene signals positioned at the edges of chromosome territories . Moreover, when RNA from specific genes are visualised by FISH, it is always seen outwith chromosome territories in a chromatin-free space (see Clemson and Lawrence 1996;Zirbel et al 1993;Bridger et al 2005). However, when other groups have assessed the nuclear distribution of all active sites of transcription by incorporation of bromo-uridine (BrUTP) in mammalian cells or the distribution of transcription factors, transcription sites have been found deep within the chromosome territories (Verschure et al 1999(Verschure et al , 2002Sadoni and Zink 2004).…”

Section: Interchromosomal Domain Compartment and Chromatin Loopsmentioning

confidence: 99%

The genome and the nucleus: a marriage made by evolution

2005

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Genomes are housed within cell nuclei as individual chromosome territories. Nuclei contain several architectural structures that interact and influence the genome. In this review, we discuss how the genome may be organised within its nuclear environment with the position of chromosomes inside nuclei being either influenced by gene density or by chromosomes size. We compare interphase genome organisation in diverse species and reveal similarities and differences between evolutionary divergent organisms. Genome organisation is also discussed with relevance to regulation of gene expression, development and differentiation and asks whether large movements of whole chromosomes are really observed during differentiation. Literature and data describing alterations to genome organisation in disease are also discussed. Further, the nuclear structures that are involved in genome function are described, with reference to what happens to the genome when these structures contain protein from mutant genes as in the laminopathies.

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“…Specific chromatin domains also occupy distinct, spatial positions, the pattern of which has been associated with the transcriptional state of the cell (Borden and Manuelidis 1988;Janevski et al 1995Janevski et al , 1997Naegele et al 1995). This led to the concept that one level of control of gene expression is exerted by spatially coupling those components that participate in transcription and RNA processing (Jackson 1991;Clemson and Lawrence 1996). It is unknown, however, whether such compartments are randomly distributed within the three-dimensional (3-D) nuclear space or are organized into a specific, non-random topology.…”

Section: Introductionmentioning

confidence: 99%

A specific conformation of the territory of chromosome 17 locates ERBB - 2 sequences to a DNase-hypersensitive domain at the nuclear periphery

Park

Boni

1998

Chromosoma

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Chromatin in interphase nuclei exhibits a topology that is associated with the transcriptional state of cells. We examined the spatial, intranuclear distribution of chromosome 17 and the ERBB-2 (HER2/neu) sequence thereon, relative to that of DNase-hypersensitive chromatin (DHC), in breast tumour cells exhibiting different levels of expression of ERBB-2. These sequences were specifically associated with the nuclear periphery, within a band of DHC. The remainder of the chromosome 17 mass showed no preferential position within the nucleus. The peripheral placement of ERBB-2 sequences is associated with a specific conformation of chromosome 17. We propose that the conformational organization of chromosome territories might represent a fundamental control mechanism in gene expression.

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Multifunctional compartments in the nucleus: Insights from DNA and RNA localization

Cited by 13 publications

References 28 publications

Single-cell c-myc gene expression in relationship to nuclear domains

Single-cell c-myc gene expression in relationship to nuclear domains

The genome and the nucleus: a marriage made by evolution

A specific conformation of the territory of chromosome 17 locates ERBB - 2 sequences to a DNase-hypersensitive domain at the nuclear periphery

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