Multifunctional Core@Shell Magnetic Nanoprobes for Enhancing Targeted Magnetic Resonance Imaging and Fluorescent Labeling in Vitro and in Vivo

Abstract

Core@shell magnetic nanoparticles (core@shell MNPs) are attracting widespread attention due to their enhancement properties for potential applications in hyperthermia treatment, magnetic resonance imaging (MRI), diagnostics, and so forth. Herein, we developed a facile thermal decomposition method for controllable synthesis of a superparamagnetic, monodispersed core@shell structure (Co@Mn = CoFeO@MnFeO) with uniform size distribution (σ < 5%, d ≈ 15 nm). The CoFeO core could enhance magnetic anisotropy, and the MnFeO shell could improve the magnetization value. The Co@Mn MNPs were transferred into aqueous solution with an amphiphilic polymer (labeled 2% TAMRA) and functionalized with PEG and target molecules (folic acid, FA) to fabricate multifunctional PMA-Co@Mn-PEG-FA nanoprobes. The obtained PMA-Co@Mn-PEG-FA nanoprobes exhibit good biocompatibility, high T relaxation values, and long-term fluorescence stability (at least 6 months). Our results demonstrate that the synthesized PMA-Co@Mn-PEG-FA nanoprobes can effectively enhance the targeted MRI and fluorescent labeling in vitro and in vivo. The research outcomes will contribute to the rational design of new nanoprobes and provide a promising pathway to promote core@shell nanoprobes for further clinical contrast MRI and photodynamic therapy in the near future.

Biconcave Carbon Nanodisks for Enhanced Drug Accumulation and Chemo‐Photothermal Tumor Therapy

“…A comparison of confocal and TPF images (Figure S4, Supporting Information) of 4T1 cells incubated with PBS solution as control further indicates that 4T1 cells had no fluoresce signal and the fluorescence of Figure d was generated by BCNs. The capability of BCNs for multimodal fluorescence imaging demonstrates their potential use as optical nanoprobes in biomedical imaging . In addition, BCNs were primarily localized in the cytoplasm of 4T1 cells, suggesting that they could be used as a transmembrane carrier for both drug delivery and PTT.…”

Recent Progress in Synthesis and Functionalization of Multimodal Fluorescent-Magnetic Nanoparticles for Biological Applications

“…In another study, multifunctional core@shell (CoFe 2 O 4 @MnFe 2 O 4 ) nanostructures coated with tetramethylrhodamine-5-carboxamide cadaverine labeled poly(isobutylene-alt-maleic anhydride), PEG and functionalized with folic acid for targeting demonstrated good biocompatibility, high T2 relaxation values, and long-term fluorescence stability. These fluorescent magnetic nanocomposites have been used to effectively enhance the targeted MRI and fluorescent labeling in vitro and in vivo [89].…”

Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

“…As the typical cell model, a MGC-803 cell line was applied for biocompatibility studies of IMNPs using resazurin assay in this experiment. 24,25 Briefly, 100 µL of cell medium (medium contained DMEM supplemented with 10% FBS, 0.1 mg mL −1 streptomycin and 100 U mL −1 penicillin) was seeded into 96-well plates at a cell density of 1 × 10 5 mL −1 . The cells were cultured overnight at 37°C (5% CO 2 ).…”