Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Wu, Yanqi; Hui, Chi Wai; Zhu, Chen; Nie, Libo; Liu, Bin; He, Nongyue

doi:10.1166/jnn.2016.12583

Cited by 9 publications

(4 citation statements)

References 36 publications

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“…Finally, several manufacturers already developed other advanced specific systems [23e28] and investigations showed their easiness, rapidity and compatibility with automation [29,30].…”

Section: Nucleic Acid Amplification Tests (Naats)mentioning

confidence: 99%

Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results

Caruana¹,

Croxatto²,

Coste³

et al. 2020

Clinical Microbiology and Infection

155

165

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Background: To face the current COVID-19 pandemic, diagnostic tools are essential. It is recommended to use real-time RT-PCR for RNA viruses in order (a) to perform a rapid and accurate diagnostic, (b) to guide patient care and management and (c) to guide epidemiological strategies. Further studies are warranted to define the role of serological diagnosis and a possible correlation between serological response and prognosis. Objectives: The aim was to guide clinical microbiologists in the use of these diagnostic tests and clinicians in the interpretation of their results. Sources: A search of literature was performed through PubMed and Google Scholar using the keywords SARS-CoV-2, SARS-CoV-2 molecular diagnosis, SARS-CoV-2 immune response, SARS-CoV-2 serology/ antibody testing, coronavirus diagnosis. Content: The present review discusses performances, limitations and use of current and future diagnostic tests for SARS-CoV-2. Implications: Real-time RT-PCR remains the reference method for diagnosis of SARS-CoV-2 infection. On the other hand, notwithstanding its varying sensitivity according to the time of infection, serology represents a valid asset (a) to try to solve possible discrepancies between a highly suggestive clinical and radiological presentation and negative RT-PCR, (b) to solve discrepancies between different PCR assays and (c) for epidemiological purposes.

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“…Finally, several manufacturers already developed other advanced specific systems [23e28] and investigations showed their easiness, rapidity and compatibility with automation [29,30].…”

Section: Nucleic Acid Amplification Tests (Naats)mentioning

confidence: 99%

Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results

Caruana¹,

Croxatto²,

Coste³

et al. 2020

Clinical Microbiology and Infection

155

165

View full text Add to dashboard Cite

show abstract

“…Wu [106] demonstrates the ease of MB automation by designing a new type of automatic sample processing device based on magnetic separation to isolate and extract both DNA and RNA. This device enables sample preparation to be completed in the field without transferring the plate to any additional equipment.…”

Section: Nucleic Acid Extractionmentioning

confidence: 99%

“…To increase throughput for NGS, MNPs are widely used to process samples. Wu et al [106] completed the preparation of 96-well plate samples with MNPs, meeting the needs of high-throughput sample preparation. Baker [166] prepared NGS libraries for constructing CFTR gene amplicon library using MiSeqDx IUO CF analysis reagent (Illumina), and used MBs for normalizing the libraries prior to pooling.…”

Section: Ngs Library Preparationmentioning

confidence: 99%

Application of magnetic nanoparticles in nucleic acid detection

Tang

et al. 2020

J Nanobiotechnol

Self Cite

201

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Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infection, drug development, and clinical diagnosis. Gene detection has a wide range of applications, such as environmental, biomedical, pharmaceutical, agriculture and forensic medicine to name a few. Compared with Sanger sequencing, high-throughput sequencing technology has the advantages of larger output, high resolution, and low cost which greatly promotes the application of sequencing technology in life science research. Magnetic nanoparticles, as an important part of nanomaterials, have been widely used in various applications because of their good dispersion, high surface area, low cost, easy separation in buffer systems and signal detection. Based on the above, the application of magnetic nanoparticles in nucleic acid detection was reviewed.

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“…with strong practicability and a number of loci can be varied simultaneously detected, [62][63][64][65][66][67][68][69][70][71][72] based on a new SNP detection approach established at our laboratory.…”

Section: Introductionmentioning

confidence: 99%

Design and Implementation of Experimental Consumable Management Device for the Automatic Nucleic Acid Detection System

Jun¹,

Zhu²,

Wang³

et al. 2016

j nanosci nanotechnol

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A management device for experimental consumable based on the STM32 is designed to improve the efficiency of nucleic acid detection and its application for the automatic nucleic acid detection system based on magnetic nanoparticles is checked. The STM32 controller is used as the core of the management control system, the workflow and classification management of the experimental consumables is realized by STM32 controlling. The positioning of the consumables is controlled with the help of photoelectric sensor, which makes the device a closed loop control system. When received instructions from the host computer through RS485 bus, the device can analyze protocols and take appropriate measures such as pushing, recycling and recording experimental consumables. A single channel of cabinet structure is used as the primary structure of the experimental consumable management to save the space of the storage and improve the efficiency of the consumable management. This device provides researchers with quick and simple centralized control of diverse experimental consumables, which has the advantages of intelligence, remote, without human intervention, etc.

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Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Cited by 9 publications

References 36 publications

Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results

Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results

Application of magnetic nanoparticles in nucleic acid detection

Design and Implementation of Experimental Consumable Management Device for the Automatic Nucleic Acid Detection System

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