Multifunctional Role of the Whey Culture Medium in the Spray-Drying Microencapsulation of Lactic Acid Bacteria

Abstract

SUMMARYThis study aims to evaluate the multifunctional role of whey culture medium during the spray drying microencapsulation of Lactobacillus fermentum K73. Whey culture medium containing growing microorganisms served to hydrate different mixtures (gum arabic, maltodextrin and whey). We evaluated the use of these mixtures as carbon sources and their protective effects on simulated gastrointestinal conditions. The optimal mixture was spray-dried while varying the outlet temperature and atomizing pressure using a response surface design. These conditions served to evaluate microorganism survival, tolerance to gastrointestinal conditions in vitro, physicochemical properties, morphometric features and stability at 4, 25 and 37 °C. Lactobacillus fermentum K73 replicated in the carrier material. Bacterial change cycles were (–1.97±0.16) log CFU/g after the drying process and (–0.61±0.08) and (–0.23±0.00) log CFU/g after exposure of the capsules to simulated gastric pH and bile salt content, respectively. The physicochemical properties and morphometric features were within the normal ranges for a powder product. The powder was stable at a storage temperature of 4 °C. The spray drying of the whey culture medium with growing microorganisms using the optimized drying conditions was successful. This study demonstrates the use of whey culture medium as a component of carrier material or as the carrier material itself, as well as its protective effects during drying, under simulated gastrointestinal conditions, and at varied storage temperatures.

Limosilactobacillus fermentum from buffalo milk is suitable for potential biotechnological process development and inhibits Helicobacter pylori in a gastric epithelial cell model

“…Further downstream processing was performed to simulate a complete manufacturing cycle. Biomass concentration on hollow fibers was the most critical point of the downstream process [4] . Clogging of the membrane, either due to production of EPS, or to the lumen of the hollow fibers, allowed processing of about 11 L of broth.…”

“…However, very few scientific publications are available on the development of fermentation processes for the large-scale production [ 2 , 19 ]. L. fermentum was demonstrated to grow on renewable feedstocks such as starch [11] , whey [4] , cereal-based substrates [13] and even anaerobic digester sludge [32] . Nevertheless, most studies and patents still use De Man, Rogosa and Sharpe (MRS) medium for bioreactor experiments [ 28 , 33 , 52 ] even if this medium is not well suited for commercial food or pharmaceutical production processes due to presence of animal derived components (beef extract and peptone).…”

Encapsulation of Lactobacillus fermentum K73 by Refractance Window drying

“…The carrier material was fixed according to the reported by Aragon-Rojas et al . 55 , where an optimization of the carrier material content was developed. The mixture was homogenized with magnetic stirrer during 30 minutes at 130 rpm.…”

Enhancement of Live Food Nutritional Status with Essential Nutrients for Improving Aquatic Animal Health: A Review

“…The spray drying method of whey culture medium with 10 6 CFU/g L. plantarum was applied. The whey culture medium is often used as a carrier material for probiotic microencapsulation and generally contains lactose and soluble proteins [ 179 ]. However, a subsequent study found that enrichment of rotifers with two LAB strains between 10 7 and 2 × 10 7 CFU/mL once a day was enough to protect the consuming fish larvae from Vibrio sp.…”