Multigenerational effects of neonicotinoids (acetamiprid, clothianidin) on growth, fertility and motility of nematode &lt;i&gt;C. elegans&lt;/i&gt;

Sakaguchi, Yuko; Mikami, Saki; Ikoma, Naoko; Kawazoe, Sadahiro; Uchida, Masaya; Tominaga, Nobuaki; Arizono, Koji; Ichikawa, Nobuhiro

doi:10.2131/fts.9.95

Cited by 2 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Toxicity data from C. elegans could be relevant to human development and diseases. To date, we have examined the effects of various chemical substances using C. elegans (Sakaguchi et al, 2022a(Sakaguchi et al, , 2022b(Sakaguchi et al, , 2022c.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of neurotoxicity of anticancer drugs using nematode Caenorhabditis elegans as a model organism

Sakaguchi,

Mizukami,

Hiroka

et al. 2023

J. Toxicol. Sci.

Self Cite

View full text Add to dashboard Cite

It is well established that platinum-based drugs, including oxaliplatin (L-OHP) and cisplatin (CDDP), as well as microtubule inhibitors paclitaxel (PTX) and vincristine (VCR), are associated with chemotherapy-induced peripheral neuropathy (CIPN). In this study, we examined and compared the characteristics of neuropathies induced by L-OHP, CDDP, PTX, and VCR to evaluate whether Caenorhabditis elegans (C. elegans) could serve as a model organism for human CIPN. Worms were cultured on nematode growth medium plates, and L1 larvae synchronized by gel filtration were employed. We then performed bioassays and examined motility. In the motility test, exposure was performed for 2, 24, and 48 hr, and time-dependent effects were measured for each exposure time and 24 hr after terminating exposure. Herein, we observed that L-OHP and CDDP exerted concentration-dependent effects above a certain concentration, and PTX and VCR exerted concentration-dependent negative effects in the bioassay. Motility recovered in L-OHP-, PTX-, and VCR-treated worms on terminating exposure. However, CDDP exposure tended to reduce motility even 24 hr after terminating exposure. L-OHP exposure could decrease motility 2 hr after exposure, with a trend toward recovery 24 hr after terminating drug exposure. The findings of the present study revealed that C. elegans could exhibit neuropathy characteristics suggested to be similar to those observed in humans, indicating that this organism could be a suitable model to explore human CIPN.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of neurotoxicity of anticancer drugs using nematode Caenorhabditis elegans as a model organism

Sakaguchi,

Mizukami,

Hiroka

et al. 2023

J. Toxicol. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

The effects on growth and reproductive function by parabens in C. elegans

Sakaguchi

Hirota

Fukushima

et al. 2022

Fundam. Toxicol. Sci.

Self Cite

View full text Add to dashboard Cite

In recent years, paraoxybenzoic acid esters (parabens) have been used in pharmaceuticals, cosmetics, and food additives. Parabens have been reported to have a weak estrogenic effect in in vitro test systems, and it is presumed that the longer the alkyl chain of the paraben, the greater its endocrine-disrupting and reproductive function effects. However, the effects of parabens on human health are still unclear. In this study, we evaluated the effects of six parabens (methyl p-hydroxybenzoate [MP], ethyl p-hydroxybenzoate [EP], propyl p-hydroxybenzoate [PP], isopropyl p-hydroxybenzoate [IPP], butyl p-hydroxybenzoate [BP], and isobutyl p-hydroxybenzoate [IBP]) on the reproductive function of the model organism Caenorhabditis elegans. We used 25, 50, and 100 µg/mL solutions of parabens in 0.1% dimethyl sulfoxide (DMSO). Bioassays (growth and maturation effect tests and reproduction effect tests) were performed on L1 larvae of wild-type C. elegans. In the growth effects test, all parabens were observed to have no effect. In the maturation effects test, there was a significant decrease in maturity at each concentration of five of the six parabens, with the exception being MP. In the reproduction effects test, a significant decrease in the number of lifetime offspring was observed at each concentration of five of the six parabens, with the exception being EP. This decrease was remarkable with PP, which has been reported to adversely affect reproductive function in rats. It is necessary to continue to focus on the estrogen-like action of parabens, including PP, and perform genetic analyses, such as RNA sequencing.

show abstract

Multigenerational effects of neonicotinoids (acetamiprid, clothianidin) on growth, fertility and motility of nematode <i>C. elegans</i>

Cited by 2 publications

References 27 publications

Evaluation of neurotoxicity of anticancer drugs using nematode <i>Caenorhabditis elegans</i> as a model organism

Evaluation of neurotoxicity of anticancer drugs using nematode <i>Caenorhabditis elegans</i> as a model organism

The effects on growth and reproductive function by parabens in <i>C. elegans</i>

Contact Info

Product

Resources

About