Multilaboratory Study of the Biomic Automated Well-Reading Instrument versus MicroScan WalkAway for Reading MicroScan Antimicrobial Susceptibility and Identification Panels

Fader, Robert; Weaver, Emily S.; Fossett, Rhonda; Toyras, Michele; Vanderlaan, John; Gibbs, David L.; Wang, Andrew; Thierjung, Nikolaus

doi:10.1128/jcm.03088-12

Cited by 45 publications

(86 citation statements)

References 6 publications

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“…The biochemical studies of P. fluorescens were performed on MicroScan Walk-Away ® [22]. Biochemical reactions pattern were carried out in all the tested group using 33 biochemicals viz.…”

Section: Biochemical Studiesmentioning

confidence: 99%

“…Antimicrobial sensitivity pattern of P. fluorescens in each group was carried out with the help of automated instrument, MicroScan WalkAway ® using NBPC30 panel, as per the manufacturer's instructions [22]. MIC and the qualitative susceptibility pattern like resistant (R), intermediate (I), or susceptible (S), were determined by observing the lowest antimicrobial concentration showing growth inhibition.…”

Section: Antimicrobial Sensitivity Assaymentioning

confidence: 99%

“…The biotype number of P. fluorescens was determined by automated MicroScan Walk-Away ® processed panel data utilizing biochemical reactions [22].…”

Section: Biotype Numbermentioning

confidence: 99%

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Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

Trivedi¹,

Branton²

2015

Clin Med Biochemistry Open Access

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Biofield therapies have been reported to improve the quality of life as compared to other energy medicine. The aim of the study was to evaluate the impact of Mr. Trivedi's biofield energy treatment on Pseudomonas fluorescens (P. fluorescens) for antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number. P. fluorescens cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 49838) number and divided in control and treated group. The effect was evaluated on day 10, and 159 after biofield treatment in lyophilized state. Further study was performed on day 5, 10, and 15 after retreatment on day 159 in revived state as per study design. All experimental parameters were studied using automated MicroScan Walk-Away ® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. fluorescens with other bacterial species after treatment. The results showed improved sensitivities and decreased MIC value of aztreonam, cefepime, moxifloxacin, and tetracycline in revived and lyophilized treated sample with respect to the control. Arginine, cetrimide, kanamycin, and glucose showed altered biochemical reactions after biofield treatment with respect to control. Biotype numbers were altered along with species in lyophilized as well as in revived group. Based on nucleotides homology and phylogenetic analysis using 16S rDNA gene sequencing, treated sample was detected to be Pseudomonas entomophila (GenBank Accession Number: AY907566) with 96% identity of gene sequencing data, which was nearest homolog species to P. fluorescens (Accession No. EF672049). These findings suggest that Mr. Trivedi's unique biofield treatment has the capability to alter changes in pathogenic P. fluorescens even in the lyophilized storage condition and can be used to modify the sensitivity of microbes against antimicrobials.

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“…The biochemical studies of P. fluorescens were performed on MicroScan Walk-Away ® [22]. Biochemical reactions pattern were carried out in all the tested group using 33 biochemicals viz.…”

Section: Biochemical Studiesmentioning

confidence: 99%

Section: Antimicrobial Sensitivity Assaymentioning

confidence: 99%

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Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

Trivedi¹,

Branton²

2015

Clin Med Biochemistry Open Access

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“…Preparation of NBPC 30 panel, inoculum followed by dehydration and rehydration were performed in a similar way as mentioned in antimicrobial susceptibility assay for analysis of biochemical reactions followed by biotype number. The detailed experimental procedures and conditions were followed as per the manufacturer's instructions [32].…”

Section: Biochemical Reaction Studiesmentioning

confidence: 99%

“…The detailed experimental procedure and conditions were followed as per the manufacturer's instructions. The antimicrobial susceptibility pattern (S: Susceptible, R: Resistant; and I: Intermediate) and MIC values were determined by observing the lowest antimicrobial concentration showing inhibition of growth [32].…”

Section: Antimicrobial Susceptibility Testmentioning

confidence: 99%

Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

Trivedi¹,

Branton²

2015

J Clin Med Genomics

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Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes. First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were studied using automated MicroScan Walk-Away ® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treated cells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant to susceptible after biofield treatment, with support of decreased MIC value (>8 to ≤ 4 µg/mL) by two-fold in all the treated samples as compared to the control. Biochemical reactions also showed significant (42.42%) alteration in the treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA (53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus) on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with species were substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and (53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showed that the identified microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. The nearest homolog genus-species of C. braakii was found to be Citrobacter werkmanii (Accession No. AF025373). The results suggested that biofield treatment has a significant impact on C. braakii in lyophilized as well as revived state. Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen.

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Automated and Manual Systems for Antimicrobial Susceptibility Testing of Bacteria

Evangelista

Karlowsky

2016

Manual of Commercial Methods in Clinical Microbiology

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Multilaboratory Study of the Biomic Automated Well-Reading Instrument versus MicroScan WalkAway for Reading MicroScan Antimicrobial Susceptibility and Identification Panels

Cited by 45 publications

References 6 publications

Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

Automated and Manual Systems for Antimicrobial Susceptibility Testing of Bacteria

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