Multilayer membranes via layer-by-layer deposition of PDDA and DNA with Au nanoparticles as tags for DNA biosensing

Zhu, Chen; Chen, Miao; Fan, Hao; Zhao, Kun; Zhuang, Shuqi; He, Pingang; Fang, Yuzhi

doi:10.1016/j.electacta.2007.11.004

Cited by 23 publications

(11 citation statements)

References 25 publications

(30 reference statements)

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“…They can be used to differentiate one base mismatch sequences from the complementary target sequences sensitively. These biosensors also do not require any additional preparation step [19,20]. Arora et al [20] studied DNA hybridization using polypyrrole-polyvinyl sulfonate modified Pt electrode.…”

Section: Introductionmentioning

confidence: 98%

Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

et al. 2010

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Poly(vinylferrocenium) (PVF ? ) modified gold (Au) electrode was developed in this study for the electrochemical sensing of deoxyribonucleic acid (DNA) hybridization based on the oxidation signals of polymer and guanine, and also for the electrochemical investigation of interaction of anticancer drug, mitomycin C (MC) and DNA immobilized onto PVF ? modified Au electrode. PVF ? modified Au electrode was prepared by electrooxidation of poly(vinylferrocene) PVF at ?0.7 V versus Ag/AgCl reference electrode. The polymer modified electrode and DNA immobilized polymer modified electrode were characterized by X-ray photoelectron (XPS), Fourier transform infrared-attenuated total reflentance (FTIR-ATR) and alternating current (AC) impedance spectroscopy. For application studies, differential pulse voltammetry (DPV) technique was used.

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Section: Introductionmentioning

confidence: 98%

Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

et al. 2010

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“…The central layer of the scaffolds was modified by incubating poly(lactide-co-glycolide) (PLG) microspheres with a range of polymers, including polyethylenimine (PEI), poly(L-lysine) (PLL), poly(allylamine hydrochloride) (PAH), polydiallyldimethylammonium (PDDA), and polydopamine (PD). These components were selected based on their ability to bind DNA, their ability to provide surface coatings on PLG, or their use in layer-by-layer assemblies [5, 6, 10–17]. The retention of DNA within the scaffold and release profile was initially characterized.…”

Section: Introductionmentioning

confidence: 99%

The contribution of plasmid design and release to in vivo gene expression following delivery from cationic polymer modified scaffolds

et al. 2010

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Tissue engineering scaffolds capable of gene delivery can provide a structure that supports tissue formation while also inducing the expression of inductive factors. Sustained release strategies are hypothesized to maintain elevated plasmid concentrations locally that can enhance gene transfer. In this report, we investigate the relationship between plasmid release kinetics and the extent and duration of transgene expression. Scaffolds were fabricated from polymer microspheres modified with cationic polymers (polyethylenimine, poly(L-lysine), poly(allylamine hydrochloride), polydiallyldimethylammonium) or polydopamine (PD), with PD enhancing incorporation and slowing release. In vivo implantation of scaffolds into the peritoneal fat pad had no significant changes in the level and duration of transgene expression between PD and unmodified scaffolds. Control studies with plasmid dried onto scaffolds, which exhibited a rapid release, and scaffolds with extended leaching to reduce initial quantities released had similar levels and duration of expression. Changing the plasmid design, from a cytomegalovirus (CMV) to an ubiquitin promoter substantially altered the duration of expression. These studies suggest that the initial dose released and vector design affect the extent and duration of transgene expression, which may be sustained over several weeks, potentially leading to numerous applications in cell transplantation and regenerative medicine.

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“…In view of the fact that their unique physical and chemical properties, such as catalytic properties, large surface area, pore structure, embedded effect, and size effect, nanomaterials have potential applications in electronics, optics, genomics, and bioanalytical effects. One of the key points is the development of catalytic labels that amplify the detection of the duplex generated between the probe DNA sequence and the target DNA sequence [37][38][39]. The use of metallic nanoparticles, semiconductor based nanoparticles and metal oxide nanoparticles are in increasing demand for enhancing biosensor response for this purpose.…”

Section: Nanoparticles (Nps)mentioning

confidence: 99%

The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization

Karadeniz¹,

Kuralay²,

Abacı³

et al. 2011

CAC

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Electrochemical biosensors have a key importance in many areas including clinical, biological, pharmaceutical, forensic, environmental and agricultural applications. They have the advantages of rapid, easy and low cost preparation allowing the localization of biocompound on electrodes of any size with independence of sample turbidity, high sensitivity and high selectivity. Electrochemical devices have attracted considerable interest in the development of DNA hybridization biosensors. They rely on the conversion of hybridization event into a direct electrochemical signal. Many efforts have been carried out in the detection of DNA hybridization for improving the stability, reproducibility and sensitivity.This review summarizes the advanced electrochemical methodologies involved in nucleic acid hybridization performed by using polymer technology, nanoparticles (NPs), magnetic particles, and carbon nanotubes (CNTs). The preparation of polymer modified electrodes especially including conducting polymers and their applications for DNA hybridization are given with the advantages of electrocatalysis, good electroactivity, high sensitivity and providing suitable matrix for DNA immobilization. The role of various nanomaterials in biosensors with the advantages of high surface area, fast heterogeneous electron transfer, excellent biocompatibility and enhanced signal with a good selectivity are presented. The combination of these techniques is also discussed in details.

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Multilayer membranes via layer-by-layer deposition of PDDA and DNA with Au nanoparticles as tags for DNA biosensing

Cited by 23 publications

References 25 publications

Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

The contribution of plasmid design and release to in vivo gene expression following delivery from cationic polymer modified scaffolds

The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization

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