Multilineage potential research on pancreatic mesenchymal stem cells of bovine

Gao, Fan; Wu, Yangnan; Wen, Hebao; Zhu, Wanwan; Ren, Han; Guan, Weijun; Tian, Xin

doi:10.1016/j.tice.2018.12.001

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…In the present study, the ability of three types of MSC to differentiate into pancreatic β-like cells in vitro was compared. Other studies reported the use of diverse methods to differentiate MSCs toward a pancreatic cell lineage (30)(31)(32)(33). A three-step method to effectively induce MENSCs, UCMSCs and DPSCs to differentiate into pancreatic β-like cells in vitro was developed.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of three types of mesenchymal stem cell to differentiate into pancreatic β‑like cells in vitro

Wang

Gao

et al. 2021

Exp Ther Med

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Type 1 diabetes (TID) is a chronic metabolic disease where the body produces insufficient or no insulin. Stem cells with multi-directional differentiation potential are transplanted and differentiate into β-like cells in vivo to replace pancreatic β cells, which has become a novel treatment strategy. The aim of the present study was to investigate the ability of three types of adult mesenchymal stem cell (MSC) to differentiate into pancreatic β-like cells in vitro in order to identify suitable sources for the treatment of diabetes. The three MSC types were menstrual blood-derived MSCs (MENSCs), umbilical cord-derived MSCs (UCMSCs) and dental pulp MSCs (DPSCs). The differentiation method used in the present study was divided into three steps and the MSCs were differentiated into pancreatic β-like cells in vitro. Among these MSCs, MENSCs had a greater ability to differentiate into islet β-like cells in vitro, while UCMSCs and DPSCs exhibited a similar differentiation potency, which was relatively lower compared with that of MENSCs. The present results indicated that MENSCs may be a suitable cell source for the curative treatment of TID.

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Section: Discussionmentioning

confidence: 99%

Comparative study of three types of mesenchymal stem cell to differentiate into pancreatic β‑like cells in vitro

Wang

Gao

et al. 2021

Exp Ther Med

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“…KMSCs were rinsed with PBS for 3 times. 10% goat serum closed cells: each well was added 500 µL 10% goat serum at room temperature for 30 min [27]. Incubation of primary antibodies: anti-VIM, anti-cd90, anti-cd109 and anti-pax2 were incubated at 4˚C overnight or at room temperature for 1 h. The KMSCs were cleaned by PBS for 3 times.…”

Section: Methodsmentioning

confidence: 99%

“…Two weeks later, the adipogenic cells [28] were xed with 4% paraformaldehyde and detected by Oil Red O staining. Furthermore, the speci c genes (Peroxisome proliferator-activated receptor-γ: PPAR-γ; Lipoprotein lipase: LPL) [27]of adipogenic cells were detected by RT-PCR.…”

Section: Differentiation Capacity Of Kmscsmentioning

confidence: 99%

“…The P6 KMSCs were inoculated in a 60 mm petri dish at a density of 0.5×10 5 cells/well. When the degree of cell fusion reached 60-70%, the complete medium was replaced with osteogenic cell induction solution [27] (DMEM/F12, 10% FBS, 10 4 IU/mL penicillin/streptomycin, 10 mM β-glycerol phosphate, 10 mM dexamethasone and 0.1 mM L-ascorbate-2phosphate). Morphological changes of the cells were observed daily.…”

Section: Differentiation Capacity Of Kmscsmentioning

confidence: 99%

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Kidney Mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats

LIANG¹,

Yan²,

Zhang³

et al. 2023

Tissue and Cell

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“…The P5 KMSCs were seeded in 60 mm plates at a density of 0.5×10 5 cells/well. When the degree of cell fusion reached 60-70%, the complete medium was replaced with adipogenic cells induction solution (DMEM/F12, 10% FBS, 100 µg/mL streptomycin, 10 4 IU/mL penicillin, 1 mM dexamethasone, 200 µM indomethacin, 0.5 mM IBMX and 10 µM insulin) [27]. Morphological changes of the cells were observed daily.…”

Section: Differentiation Capacity Of Kmscsmentioning

confidence: 99%

Kidney mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats

LIANG

Yan

Zhang

et al. 2022

Preprint

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This experiment was designed to demonstrate that mesenchymal stem cells derived from kidney can alleviate cisplatin-induced kidney injury and renal cell apoptosis through paracrine pathway. Firstly, mesenchymal stem cells were isolated from kidney of young rats, and their surface-specific markers were identified by RT-PCR and immunofluorescence staining. Self-renewal ability of renal mesenchymal stem cells was observed by cell counting and BrdU fluorescence staining. KMSCs at logarithmic growth stage were traced and injected into rat kidney injury model induced by cisplatin through tail vein injection. The results showed that KMSCs homed in the kidney tissues, decreased the secretion of inflammatory factors (CRP, TNFα, IL-1β, IL-6), and alleviated renal function. H＆E, Masson and PASM staining showed that KMSCs could alleviate pathological damage in rats. TUNEL assay showed that KMSCs could reduce the apoptosis of rat kidney cells induced by cisplatin. Finally, IHC results showed that cisplatin could induce higher expression of the pro-apoptotic protein Bax and lower expression of anti-apoptotic Bcl-2 in kidney tissues. However, KMSCs could reverse the pro-apoptotic effect of cisplatin on kidney cells and improve the survival rate of rats. In conclusion, KMSCs were successfully isolated in this study, and KMSCs have therapeutic effects on rat kidney injury induced by cisplatin. The therapeutic effect can be achieved through anti-inflammation, anti-apoptosis and repair of renal function. The results of cell tracer showed that KMSCs could inhibit apoptosis and repair renal injury by endocrine or paracrine mechanism.

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Multilineage potential research on pancreatic mesenchymal stem cells of bovine

Cited by 11 publications

References 29 publications

Comparative study of three types of mesenchymal stem cell to differentiate into pancreatic β‑like cells in vitro

Comparative study of three types of mesenchymal stem cell to differentiate into pancreatic β‑like cells in vitro

Kidney Mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats

Kidney mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats

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