Multilocus genotyping of Giardia duodenalis in Tibetan sheep and yaks in Qinghai, China

Jin, Yue; Fei, Jilan; Cai, Jinzhong; Wang, Xiaolan; Li, Na; Guo, Yaqiong; Feng, Yaoyu; Xiao, Lihua

doi:10.1016/j.vetpar.2017.09.021

Cited by 37 publications

(42 citation statements)

References 37 publications

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“…Sequence analysis of 51 G . duodenalis positive samples showed mono-infection with the ruminant-specific assemblage E at the three loci, which is similar to the results of previous investigations on adult goats [ 8 , 10 , 11 , 29 ]. According to previous studies, the zoonotic genotypes A and B have been detected in goats, which suggests a zoonotic threat to human beings [ 12 ].…”

Section: Resultssupporting

confidence: 90%

“…Among the bg , tpi , and gdh loci, predominant subtypes of assemblages E at each locus (E5 (94.1%) at the bg locus, E2 (97.7%) at the tpi locus, and E4 (97.0%) at the gdh locus) were also commonly detected in sheep, cattle, yaks, and pigs, demonstrating a potential risk of cross-species transmission of G . duodenalis to different animals [ 27 , 29 , 31 , 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…Compared with other MLGs detected in previous studies, lower genetic heterogeneity was observed (only 3 MLGs detected) in G . duodenalis , which was mainly reflected by the limited subtype diversity of assemblage E with only 4, 2, and 2 subtypes yielded at the bg , gdh , and tpi loci, respectively [ 29 , 33 ]. Phylogenetic analysis indicated that the three novel assemblage E MLGs were genetically distinct from those isolated from sheep in Qinghai and Henan Provinces, China [ 29 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

“…duodenalis , which was mainly reflected by the limited subtype diversity of assemblage E with only 4, 2, and 2 subtypes yielded at the bg , gdh , and tpi loci, respectively [ 29 , 33 ]. Phylogenetic analysis indicated that the three novel assemblage E MLGs were genetically distinct from those isolated from sheep in Qinghai and Henan Provinces, China [ 29 , 31 ]. Moreover, MLG-E1 (sc) and MLG-E3 (sc) were placed in the major cluster of MLGs from Tibetan sheep in Qinghai, whereas MLG-E2 (sc) was clustered with MLGs from sheep in Henan ( Fig 2 ).…”

Section: Resultsmentioning

confidence: 99%

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Occurrence and genetic characterization of Giardia duodenalis and Cryptosporidium spp. from adult goats in Sichuan Province, China

Zhong

et al. 2018

PLoS ONE

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Giardia duodenalis and Cryptosporidium spp. are common gastrointestinal protozoa in mammals. Many studies have been conducted on the distribution of G. duodenalis and Cryptosporidium spp. genotypes in sheep and cattle. However, in China, information about molecular characterization and genetic analysis of G. duodenalis and Cryptosporidium spp. in goats is limited. In this study, 342 fecal samples from adult goats were collected from 12 farms in Sichuan Province, China. The occurrence of G. duodenalis and Cryptosporidium spp. in adult goats was 14.9% (51/342) and 4.7% (16/342), respectively. All G. duodenalis were identified as assemblage E, with two novel genotypes (assemblages E17 and E18) being detected at the beta-giardin (bg) locus. Based on three loci—beta-giardin (bg), triose phosphate isomerase (tpi), and glutamate dehydrogenase (gdh)—multilocus sequence typing revealed three novel multilocus genotypes (MLGs) of assemblage E (MLG-E1, E2, E3 (sc)). Small Subunit (SSU) rRNA-based PCR identified two Cryptosporidium species, namely C. xiaoi (11/16) and C. suis (5/16). This study is not only the first to report C. suis infection in adult goats in China but is also the first to use the MLG approach to identify G. duodenalis in adult goats.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Occurrence and genetic characterization of Giardia duodenalis and Cryptosporidium spp. from adult goats in Sichuan Province, China

Zhong

et al. 2018

PLoS ONE

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“…Giardia duodenalis (syn. Giardia intestinalis, Giardia lamblia) is a common gastrointestinal protozoon that causes enteric disease in a variety of animal species and humans [1][2][3][4]. More than 40 species of animals have been reported to be infected with G. duodenalis over the world [2,5].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genotype Distribution of Giardia duodenalis in Rabbits in Shandong Province, Eastern China

Zou

Peng

et al. 2020

BioMed Research International

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Giardia duodenalis is a zoonotic enteric parasite that can infect humans and a number of animal species including rabbits with a worldwide distribution. Infection with G. duodenalis can cause serious public health problems and significant economic losses to animal husbandry. So accurate understanding of the prevalence and genotype distribution of G. duodenalis in rabbits is necessary. In the present study, a total of 616 fecal samples were collected from rabbits in Shandong province, eastern China, and examined in G. duodenalis prevalence and genotypes by nested PCR amplification of β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) gene loci of G. duodenalis. Sixty-nine (11.2%) of the examined rabbit fecal samples were G. duodenalis-positive. Of them, the prevalence of G. duodenalis is 8.4% (41/490) in Rizhao city and 22.2% (28/126) in Weihai city. Breeds, region, and feeding modes were highly correlated with G. duodenalis infection in rabbits. Moreover, three genotypes

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Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in 1–2-month-old highland yaks in Qinghai Province, China

Wang

et al. 2018

Parasitol Res

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Cryptosporidium and Giardia are ubiquitous parasites that infect humans and animals. Few reports are available on the prevalence of these two protozoan parasites in yaks (Bos grunniens). In this study, 344 faecal samples were collected from yaks with diarrhoea in the Chenduo and Nangqian counties of Qinghai Province, China. Cryptosporidium spp. and Giardia duodenalis were detected by light and immunofluorescence microscopy and nested PCR (nPCR). Fifteen samples were positive (4.5%) by Kinyoun staining, 40 (11.6%) samples were positive by immunofluorescence test (IFT), and 39 (11.3%) samples were positive by nPCR for Cryptosporidium spp., Cryptosporidium bovis (11/39, 28.2%) was the most prevalent species, followed by C. ryanae (6/39, 15.4%), C. andersoni (5/39, 12.8%), C. struthionis (5/39, 12.8%), C. parvum (5/39, 12.8%), C. hominis (4/39, 10.3%) and C. canis (3/39, 7.7%). Thirteen out of 344 (3.8%) samples were positive for Giardia by simple microscopy, 20 (5.8%) by IFT and 18 samples (5.2%) yak faecal samples were Giardia positive by nPCR. Two G. duodenalis assemblages (B, E) were detected in this study. Nine positive samples for G. duodenalis assemblage E were from the towns of Xiewu (8/9, 4.9%) and Xiangda (1/9, 1.3%), and nine positive samples (9/9, 8.5%) for G. duodenalis assemblage B were from the town of Zhenqin. This report provides information about infection with Cryptosporidium species and G. duodenalis assemblages in domesticated 1-2-month-old highland yaks living in the Qinghai-Tibet Plateau region of China.

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Multilocus genotyping of Giardia duodenalis in Tibetan sheep and yaks in Qinghai, China

Cited by 37 publications

References 37 publications

Occurrence and genetic characterization of Giardia duodenalis and Cryptosporidium spp. from adult goats in Sichuan Province, China

Occurrence and genetic characterization of Giardia duodenalis and Cryptosporidium spp. from adult goats in Sichuan Province, China

Prevalence and Genotype Distribution of Giardia duodenalis in Rabbits in Shandong Province, Eastern China

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in 1–2-month-old highland yaks in Qinghai Province, China

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