Multilocus Sequence Typing of Corynebacterium ulcerans Provides Evidence for Zoonotic Transmission and for Increased Prevalence of Certain Sequence Types among Toxigenic Strains

König, Christina; Meinel, Dominik M.; Margos, Gabriele; Konrad, Regina; Sing, Andreas

doi:10.1128/jcm.02291-14

Cited by 35 publications

(44 citation statements)

References 24 publications

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“…The MLST method used in the present study employed the nucleotide sequencing of housekeeping genes commonly utilized for C. diphtheriae MLST , as well as in recent MLST analyses of C. ulcerans . Although we selected different nucleotide regions for identifying alleles than those used in other studies, our method had sufficient power and resolution to type 24 distinct C. ulcerans strains and isolates.…”

Section: Discussionmentioning

confidence: 99%

Toxigenic Corynebacterium ulcerans isolated from a hunting dog and its diphtheria toxin antibody titer

Katsukawa

Komiya

Umeda³

et al. 2016

Microbiology and Immunology

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Toxigenic Corynebacterium ulcerans is a zoonotic pathogen that produces diphtheria toxin and causes a diphtheria-like illness in humans. The organism is known to infect and circulate among dogs, which can then transmit it to humans. Furthermore, previous studies have found that C. ulcerans is carried by wild animals, including game animals. In the present study, we tested hunting and companion dogs for the presence of toxigenic C. ulcerans and succeeded in isolating the bacterium from a hunting dog. Moreover, several hunting dogs had serum diphtheria antitoxin titers that were higher than the titers required for protection in humans, suggesting a history of exposure to toxigenic Corynebacterium strains. Notably, ribotyping, pulsed-field gel electrophoresis and tox gene sequencing demonstrated that the isolate from the hunting dog clustered with previously characterized C. ulcerans strains isolated from wild animals, as opposed to groups of isolates from humans and companion dogs. Interestingly, the wild animal cluster also contains an isolate from an outdoor breeding dog, which could have formed a bridge between isolates from wild animals and those from companion dogs. The results presented herein provide insight into the mechanism by which the zoonotic pathogen C. ulcerans circulates among wild animals, hunting and companion dogs, and humans.

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Section: Discussionmentioning

confidence: 99%

Toxigenic Corynebacterium ulcerans isolated from a hunting dog and its diphtheria toxin antibody titer

Katsukawa

Komiya

Umeda³

et al. 2016

Microbiology and Immunology

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“…Assembly quality was checked with QUAST [55]. MLST was performed with the assembled WGS data based on the seven housekeeping loci atpA, dnaE, dnaK, fusA, leuA, odhA, rpoB of the C. diphtheriae scheme [7,8] using Ridom SeqSphere+ (Ridom, Münster, Germany) and matching to pubmlst database (http:// pubmlst. org/ cdiphtheriae/).…”

Section: Genome Featuresmentioning

confidence: 99%

“…Differentiation also succeeds through analysis of genetic characteristics, such as variations in 16S rRNA gene, rpoB gene or dispersed over the genome. Multi locus sequence typing (MLST) is one of the most common tools for molecular typing [5,6], widely used for C. diphtheriae [7] and C. ulcerans [8] and implemented for delineating species within bacterial genera [9]. Currently, whole genome sequencing (WGS) offers the highest molecular resolution and is increasingly used for typing and phylogenetic classification of various pathogens, including corynebacteria [10][11][12][13].…”

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confidence: 99%

Corynebacterium silvaticum sp. nov., a unique group of NTTB corynebacteria in wild boar and roe deer

Dangel¹,

Berger²,

Rau

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A total of 34 Corynebacterium sp. strains were isolated from caseous lymph node abscesses of wild boar and roe deer in different regions of Germany. They showed slow growth on Columbia sheep blood agar and sparse growth on Hoyle’s tellurite agar. Cellular fatty acid analysis allocated them in the C. diphtheriae group of genus Corynebacterium . MALDI-TOF MS using specific database extensions and rpoB sequencing resulted in classification as C. ulcerans . Their quinone system is similar to C. ulcerans , with major menaquinone MK-8(H2). Their complex polar lipid profile includes major lipids phosphatidylinositol, phosphatidylinositol-mannoside, diphosphatidylglycerol, but also unidentified glycolipids, distinguishing them clearly from C. ulcerans . They ferment glucose, ribose and maltose (like C. ulcerans ), but do not utilise d-xylose, mannitol, lactose, sucrose and glycogen (like C. pseudotuberculosis ). They showed activity of catalase, urease and phospholipase D, but variable results for alkaline phosphatase and alpha-glucosidase. All were non-toxigenic, tox gene bearing and susceptible to clindamycin, penicillin and erythromycin. In 16SrRNA gene and RpoB protein phylogenies the strains formed distinct brancheswith C. ulcerans as nearest relative.Whole genome sequencing revealed the unique sequence type 578, a distinctbranch in pangenomic core genome MLST, average nucleotide identities <91%, enhancedgenome sizes (2.55 Mbp) and G/C content (54.4 mol%) compared to related species.These results suggest that the strains represent a novel species, for which wepropose the name Corynebactriumsilvaticum sp. nov., based on their first isolation from forest-dwellinggame animals. The type strain isKL0182T (= CVUAS 4292T = DSM 109166T = LMG 31313T= CIP 111 672T).

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“…; Konig et al . ). The usual route of transmission to humans was from the domesticated animals and wild animals, but the possibility for human‐to‐human transmission has been reported by Konrad et al .…”

Section: Introductionmentioning

confidence: 97%

Strengthening the laboratory diagnosis of pathogenicCorynebacteriumspecies in the Vaccine era

Sekar

Veeraraghavan

Anandan

et al. 2017

Lett Appl Microbiol

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Over the last three decades, successful implementation of the diphtheria vaccination in the developed and developing countries has reduced the infections caused by the toxigenic strains of Corynebacterium diphtheriae, but a concomitant increase in the invasive infections due to the nontoxigenic strains was seen. In addition, the recent reports on the emergence of nontoxigenic toxin gene-bearing strains, having the potential to revert back to toxigenic form poses a significant threat to human beings. Besides infections caused by C. diphtheriae, the emergence of the respiratory, cutaneous and invasive infections by related pathogenic Corynebacterium species like C. ulcerans and C. pseudotuberculosis, complicate the diagnosis and management of infection. These observations together with the widespread prevalence of diphtheria in the vaccine era, necessitates the strengthening of the epidemiological surveillance and laboratory diagnosis of the pathogen. This review provides the overview of the advantages and limitations of different molecular methods and the role of MALDI-TOF in the laboratory diagnosis of Diphtheria. The contribution of next generation sequencing technology and different genotyping techniques in understanding the pathogenicity, transmission dynamics and epidemiology of the C. diphtheriae is discussed.

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Multilocus Sequence Typing of Corynebacterium ulcerans Provides Evidence for Zoonotic Transmission and for Increased Prevalence of Certain Sequence Types among Toxigenic Strains

Cited by 35 publications

References 24 publications

Toxigenic Corynebacterium ulcerans isolated from a hunting dog and its diphtheria toxin antibody titer

Toxigenic Corynebacterium ulcerans isolated from a hunting dog and its diphtheria toxin antibody titer

Corynebacterium silvaticum sp. nov., a unique group of NTTB corynebacteria in wild boar and roe deer

Strengthening the laboratory diagnosis of pathogenicCorynebacteriumspecies in the Vaccine era

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