Multimethode zur Bestimmung der R�ckst�nde von Chemotherapeutika, Antiparasitika und Wachstumsf�rderern in Lebensmitteln tierischer Herkunft

Abstract

Nitrofurans and dinitrocarbanilide are extracted from food according to the multiresidue method and detected by Reversed-phase-HPLC with UV-detection at 360 nm. Records of the UV-spectra confirm the results qualitatively. Possibilities for further confirmation are discussed. Substances in amounts below 0.01 mg/kg can only be determined with particular care. Statistical confirmation of this method and possible problems are mentioned. An interlaboratory collaborative study confirmed the method: furazolidone, furaltadone, nitrofurazone, nitrofurantoin, and nicarbazin (the dinitrocarbanilide containing component) were added to homogenized eggs in amounts of 0.005-0.150 mg/kg. The procedure can be standardized as an official analytical method at a detection limit of 0.005 mg/kg for nitrofurans and 0.01 mg/kg for nicarbazin.

Efficient HPLC method for the determination of nicarbazin, as dinitrocarbanilide in broiler liver

“…The extraction and clean-up procedure that was developed in this work offers advantages over previous HPLC-UV methods for determination of dinitrocarbanilide in liver [8,15]. Malisch developed an early method for isolation of a range of drugs from tissues using labour intensive liquid-liquid partitioning clean-up procedures [8].…”

“…Malisch developed an early method for isolation of a range of drugs from tissues using labour intensive liquid-liquid partitioning clean-up procedures [8]. Draisci et al later developed a method to isolate DNC residues from broiler tissues, eggs, feed and litter using large volumes of solvent, which required the use of rotary evaporation systems.…”

“…At the commencement of this work, extraction and cleanup procedures from published literature [8,12] were evaluated for compatibility with liver samples and HPLC-UV determination. Using these methods, late eluting peaks interfered with subsequent sample analysis.…”

“…A number of residue methods have been developed for the determination of DNC in eggs [8][9][10][11][12][13][14][15], muscle [14][15][16][17] and liver [12][13][14][15][16][17]. Published methods for determination of DNC in liver are LC-MS [11,13,14,16], HPLC [8][9][10]15,17] and Biacore TM biosensor [12] based methods. The available HPLC methods involve liquid-liquid partitioning using large volumes of organic solvent [8,15] or are MSPD (matrix solid phase dispersion) based and lack sensitivity [17].…”

“…Published methods for determination of DNC in liver are LC-MS [11,13,14,16], HPLC [8][9][10]15,17] and Biacore TM biosensor [12] based methods. The available HPLC methods involve liquid-liquid partitioning using large volumes of organic solvent [8,15] or are MSPD (matrix solid phase dispersion) based and lack sensitivity [17].…”