Multimodal capture − antibody-independent lateral flow immunoassay based on AuNF − PMBA for point-of-care diagnosis of bacterial urinary tract infections

Wu, Pengcheng; Zuo, Wanchao; Wang, Yufeng; Yuan, Qinfang; Yang, Jun; Liu, Xinmei; Jiang, Hui; Dai, Jianjun; Xue, Feng; Ju, Yanmin

doi:10.1016/j.cej.2022.139021

Cited by 38 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the pH and ionic strength of the matrix solution affect the binding process of the antibodies and nanomaterials. In contrast to the traditional LFSB model, some researchers have investigated a label-free LFSB based on the nanomaterial-bacteria-antibody sandwich model. − These special nanomaterials can not only act as recognition agents to capture target pathogenic bacteria but also provide sensing signals, thus allowing the direct detection for pathogenic bacteria without nanomaterial-labeled antibodies. In addition, this label-free mode eliminates the screening process of antibody pairs for pathogenic bacteria, which greatly saves time and reduces costs.…”

Section: Lfsbs For Direct Detection Of Foodborne Pathogenic Bacteriamentioning

confidence: 99%

Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Huang

Cheng

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Rapid and sensitive detection of foodborne pathogenic bacteria is particularly important for the prevention and control of foodborne diseases. The lateral flow strip biosensor (LFSB) is one of the most promising point-of-care detection tools and has been widely used in food safety monitoring. This review introduces recent advances in the detection of foodborne pathogenic bacteria using LFSBs. According to different bacterial biomarkers, we summarize the direct and indirect sensing strategies of bacterial LFSBs. The direct sensing strategies for whole bacterial cells are divided into antibodies, antibody alternatives, and label-free according to the recognition elements. The indirect sensing strategies refer to the detection of bacterial nucleic acids and metabolites. Next, we compare and discuss the applications of direct and indirect sensing strategies. Finally, the existing challenges, future perspectives, and development directions are discussed, which will facilitate the theoretical innovation and practical application for bacterial LFSBs.

show abstract

Section: Lfsbs For Direct Detection Of Foodborne Pathogenic Bacteriamentioning

confidence: 99%

Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Huang

Cheng

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Moreover, the ability to cross-validate between different modes further bolsters the reliability of the results. 8,9 Consequently, the development of novel LFIA sensors is imperative.…”

Section: Introductionmentioning

confidence: 99%

“…Fortunately, the emergence of novel LFIAs featuring two or more output signals, namely dual-mode LFIAs (dLFIAs) or multimode LFIAs (mLFIAs) has made it possible to break the aforementioned bottlenecks. , These novel LFIAs preserve the simplicity of colorimetric mode while incorporating quantitative modes of varying sensitivities, which can meet the detection requirements of diverse scenarios and greatly increase the sensitivity and accuracy of detection. Moreover, the ability to cross-validate between different modes further bolsters the reliability of the results. , Consequently, the development of novel LFIA sensors is imperative.…”

Section: Introductionmentioning

confidence: 99%

Dual-Mode Lateral Flow Immunoassay Based on “Pompon Mum”-Like Fe₃O₄@MoS₂@Pt Nanotags for Sensitive Detection of Viral Pathogens

Xu,

Zhao,

Lin

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Lateral flow immunoassay (LFIA) has been widely used for the early diagnosis of diseases. However, conventional colorimetric LFIA possesses limited sensitivity, and the single-mode readout signal is easily affected by the external environment, leading to insufficient accuracy. Herein, multifunctional Fe 3 O 4 @MoS 2 @Pt nanotags with a unique "pompon mum"-like structure were triumphantly prepared, exhibiting excellent peroxidase (POD)-like activity, photothermal properties, and magnetic separation capability. Furthermore, the Fe 3 O 4 @MoS 2 @Pt nanotags were used to establish dual-mode LFIA (dLFIA) for the first time, enabling the catalytic colorimetric and photothermal dual-mode detection of severe acute respiratory syndrome coronavirus 2 nucleocapsid protein (SARS-CoV-2 NP) and influenza A (H1N1). The calculated limits of detection (cLODs) of SARS-CoV-2 NP and H1N1 were 80 and 20 ng/mL in catalytic colorimetric mode and 10 and 8 ng/mL in photothermal mode, respectively, demonstrating about 100 times more sensitive than the commercial colloidal Au-LFIA strips (1 ng/mL for SARS-CoV-2 NP; 1 μg/ mL for H1N1). The recovery rates of dLFIA in simulated nose swab samples were 95.2−103.8% with a coefficient of variance of 2.3−10.1%. These results indicated that the proposed dLFIA platform showed great potential for the rapid diagnosis of respiratory viruses.

show abstract

“…Lateral flow immunoassay (LFIA) is a widely utilized tool for point-of-care (POC) detection. Multimodal LFIA technology has developed rapidly and has been successfully applied to food testing, , in vitro diagnostics, , and environmental monitoring recent years. , Multisignal LFIA sensors are constructed by integrating antibodies (monoclonal, polyclonal, and nanobody) and nanomaterials (noble metal nanomaterials, carbon nanomaterials, quantum dots and nanoenzymes, etc. ), which can provide multiple signal outputs, mainly including colorimetric signals, fluorescent signals, Raman signals, electrochemical signals and thermal signals .…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly Multivalent Fluorescence-Nanobody Coupled Multifunctional Nanomaterial with Colorimetric Fluorescence and Photothermal to Enhance Immunochromatographic Assay

Li,

Zhang,

Zhang

et al. 2023

ACS Nano

View full text Add to dashboard Cite

The multimodal lateral flow immunoassay (LFIA) has provided accurate and reliable results for fast and immediate detection. Nonetheless, multimodal LFIA remains challenging to develop biosensors with high sensitivity and tolerance to matrix interference in agro-food. In this study, we developed a self-assembled multivalent fluorescence-nanobody (Nb26-EGFP-H6) with 16.5-fold and 30-fold higher affinity and sensitivity than a monovalent nanobody (Nb26). Based on the Nb26-EGFP-H6, we synthesized enhanced immune-probes Zn-CN@Nb26-EGFP-H6 by pyrolyzing and oxidizing an imidazolating zeolite framework-8 (ZIF-8) to obtain photothermal metal–carbon nanomaterials (Zn-CN) for immobilizing Nb26-EGFP-H6. The rough and porous structure of Zn-CN with a large surface area facilitates the enrichment and immobilization of antibodies. A trimodal lateral flow immunoassay (tLFIA) with colorimetric, fluorescent, and photothermal triple signal outputs was constructed for the detection of aflatoxin B1 (AFB1) in maize. Attractively, the Zn-CN-based tLFIA’s multiplex guarantees accurate and sensitive detection of AFB1, with triple signal detection limits of 0.0012 ng/mL (colorimetric signals), 0.0094 ng/mL (fluorescent signals), and 0.252 ng/mL (photothermal signals). The sensitivity of the trimode immunosensor was 628-fold and 42-fold higher than that of the original Nb26-based ELISA (IC50) and the unimodal LFIA (LOD). This work provides an idea for constructing a sensitive, tolerant matrix and efficient and accurate analytical platform for rapidly detecting AFB1 in food.

show abstract

Multimodal capture − antibody-independent lateral flow immunoassay based on AuNF − PMBA for point-of-care diagnosis of bacterial urinary tract infections

Cited by 38 publications

References 49 publications

Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Dual-Mode Lateral Flow Immunoassay Based on “Pompon Mum”-Like Fe₃O₄@MoS₂@Pt Nanotags for Sensitive Detection of Viral Pathogens

Self-Assembly Multivalent Fluorescence-Nanobody Coupled Multifunctional Nanomaterial with Colorimetric Fluorescence and Photothermal to Enhance Immunochromatographic Assay

Contact Info

Product

Resources

About

Multimodal capture − antibody-independent lateral flow immunoassay based on AuNF − PMBA for point-of-care diagnosis of bacterial urinary tract infections

Cited by 38 publications

References 49 publications

Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Dual-Mode Lateral Flow Immunoassay Based on “Pompon Mum”-Like Fe3O4@MoS2@Pt Nanotags for Sensitive Detection of Viral Pathogens

Self-Assembly Multivalent Fluorescence-Nanobody Coupled Multifunctional Nanomaterial with Colorimetric Fluorescence and Photothermal to Enhance Immunochromatographic Assay

Contact Info

Product

Resources

About

Dual-Mode Lateral Flow Immunoassay Based on “Pompon Mum”-Like Fe₃O₄@MoS₂@Pt Nanotags for Sensitive Detection of Viral Pathogens