Multimodal imaging system combining optical coherence tomography and Brillouin microscopy for neural tube imaging

Ambekar, Yogeshwari S.; Singh, Manmohan; Schill, Alexander; Zhang, Jitao; Zevallos-Delgado, Christian; Khajavi, Behzad; Aglyamov, Salavat R.; Finnell, Richard H.; Scarcelli, Giuliano; Larin, Kirill V.

doi:10.1364/ol.453996

Cited by 21 publications

(13 citation statements)

References 31 publications

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“…Furthermore, although the ex vivo studies performed in this work provide valuable information, translating Brillouin microscopy measurements to in vivo assessment of corneal stiffness introduces measurement tradeoffs in terms of the number of samples acquired in measurement to prevent the effects of motion artifacts and to reduce subject discomfort. However, OCT guidance 52 could be used to correct the motion. Brillouin microscopy has been performed in vivo in murine embryos 52 and humans.…”

Section: Discussionmentioning

confidence: 99%

“…However, OCT guidance 52 could be used to correct the motion. Brillouin microscopy has been performed in vivo in murine embryos 52 and humans. 30 , 52 …”

Section: Discussionmentioning

confidence: 99%

“…Brillouin microscopy was performed in WT (n = 5) and Col12a1 –/– (n = 5) corneas using a home-built setup 52 consisting of a 660 nm single-mode laser source (Torus; Laser Quantum Inc., Fremont, CA, USA), with 35 mW power on the sample. The Brillouin frequency shift was detected by a spectrometer composed of a dual-stage virtually imaged phase array spectrometer 53 with 30 GHz of free spectral range.…”

Section: Methodsmentioning

confidence: 99%

“…1 a), the Brillouin system was co-aligned with a swept-source OCT system with a central wavelength of 1310 nm, bandwidth of 105 nm, and A-scan rate of 50 kHz for image-guided Brillouin measurements. 52 …”

Section: Methodsmentioning

confidence: 99%

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Multiple Optical Elastography Techniques Reveal the Regulation of Corneal Stiffness by Collagen XII

Nair

Ambekar

Zevallos-Delgado

et al. 2022

Invest. Ophthalmol. Vis. Sci.

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Purpose Collagen XII plays a role in regulating the structure and mechanical properties of the cornea. In this work, several optical elastography techniques were used to investigate the effect of collagen XII deficiency on the stiffness of the murine cornea. Methods A three-prong optical elastography approach was used to investigate the mechanical properties of the cornea. Brillouin microscopy, air-coupled ultrasonic optical coherence elastography (OCE) and heartbeat OCE were used to assess the mechanical properties of wild type (WT) and collagen XII–deficient ( Col12a1 –/– ) murine corneas. The Brillouin frequency shift, elastic wave speed, and compressive strain were all measured as a function of intraocular pressure (IOP). Results All three optical elastography modalities measured a significantly decreased stiffness in the Col12a1 –/– compared to the WT ( P < 0.01 for all three modalities). The optical coherence elastography techniques showed that mean stiffness increased as a function of IOP; however, Brillouin microscopy showed no discernable trend in Brillouin frequency shift as a function of IOP. Conclusions Our approach suggests that the absence of collagen XII significantly softens the cornea. Although both optical coherence elastography techniques showed an expected increase in corneal stiffness as a function of IOP, Brillouin microscopy did not show such a relationship, suggesting that the Brillouin longitudinal modulus may not be affected by changes in IOP. Future work will focus on multimodal biomechanical models, evaluating the effects of other collagen types on corneal stiffness, and in vivo measurements.

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Section: Discussionmentioning

confidence: 99%

“…However, OCT guidance 52 could be used to correct the motion. Brillouin microscopy has been performed in vivo in murine embryos 52 and humans. 30 , 52 …”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Multiple Optical Elastography Techniques Reveal the Regulation of Corneal Stiffness by Collagen XII

Nair

Ambekar

Zevallos-Delgado

et al. 2022

Invest. Ophthalmol. Vis. Sci.

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“…In addition, the dorsal neural tissue in early Xenopus embryo has been observed to increase almost 4 folds from stage 11.5 to stage 21 11 . Recently, the preliminary studies on mouse embryo suggest as large as an 80% increase of tissue modulus during neurulation by Brillouin microscopy 31 , 34 . Although these data are not directly comparable to this work due to the differences in species and/or the used techniques, the similar trend observed in chick embryos suggests that tissue biomechanics may play an important role during NTC across species.…”

Section: Discussionmentioning

confidence: 99%

Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy

Handler

Scarcelli

Zhang

2023

Sci Rep

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Neural tube closure (NTC) is a complex process of embryonic development involving molecular, cellular, and biomechanical mechanisms. While the genetic factors and biochemical signaling have been extensively investigated, the role of tissue biomechanics remains mostly unexplored due to the lack of tools. Here, we developed an optical modality that can conduct time-lapse mechanical imaging of neural plate tissue as the embryo is experiencing neurulation. This technique is based on the combination of a confocal Brillouin microscope and a modified ex ovo culturing of chick embryo with an on-stage incubator. With this technique, for the first time, we captured the mechanical evolution of the neural plate tissue with live embryos. Specifically, we observed the continuous increase in tissue modulus of the neural plate during NTC for ex ovo cultured embryos, which is consistent with the data of in ovo culture as well as previous studies. Beyond that, we found that the increase in tissue modulus was highly correlated with the tissue thickening and bending. We foresee this non-contact and label-free technique opening new opportunities to understand the biomechanical mechanisms in embryonic development.

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Disruption of Fuz in mouse embryos generates hypoplastic hindbrain development and reduced cranial nerve ganglia

Caiaffa,

Ambekar,

Singh

et al. 2024

Developmental Dynamics

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BackgroundThe brain and spinal cord formation is initiated in the earliest stages of mammalian pregnancy in a highly organized process known as neurulation. Environmental or genetic interferences can impair neurulation, resulting in clinically significant birth defects known collectively as neural tube defects. The Fuz gene encodes a subunit of the CPLANE complex, a macromolecular planar polarity effector required for ciliogenesis. Ablation of Fuz in mouse embryos results in exencephaly and spina bifida, including dysmorphic craniofacial structures due to defective cilia formation and impaired Sonic Hedgehog signaling.ResultsWe demonstrate that knocking Fuz out during embryonic mouse development results in a hypoplastic hindbrain phenotype, displaying abnormal rhombomeres with reduced length and width. This phenotype is associated with persistent reduction of ventral neuroepithelial stiffness in a notochord adjacent area at the level of the rhombomere 5. The formation of cranial and paravertebral ganglia is also impaired in these embryos.ConclusionsThis study reveals that hypoplastic hindbrain development, identified by abnormal rhombomere morphology and persistent loss of ventral neuroepithelial stiffness, precedes exencephaly in Fuz ablated murine mutants, indicating that the gene Fuz has a critical function sustaining normal neural tube development and neuronal differentiation.

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Multimodal imaging system combining optical coherence tomography and Brillouin microscopy for neural tube imaging

Cited by 21 publications

References 31 publications

Multiple Optical Elastography Techniques Reveal the Regulation of Corneal Stiffness by Collagen XII

Multiple Optical Elastography Techniques Reveal the Regulation of Corneal Stiffness by Collagen XII

Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy

Disruption of Fuz in mouse embryos generates hypoplastic hindbrain development and reduced cranial nerve ganglia

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