Multimodal polymer encapsulated CdSe/Fe3O4 nanoplatform with improved biocompatibility for two-photon and temperature stimulated bioapplications

Antoniak, Magda A.; Pązik, Robert; Bazylińska, Urszula; Wiwatowski, Kamil; Tomaszewska, Anna; Kulpa-Greszta, Magdalena; Adamczyk‐Grochala, Jagoda; Wnuk, Maciej; Maćkowski, Sebastian; Lewińska, Anna; Nyk, Marcin

doi:10.1016/j.msec.2021.112224

Cited by 26 publications

(23 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After HP and 5-azaC treatments, selected parameters of oxidative stress were considered such as superoxide levels, glutathione redox potential, and oxidative DNA damage. Superoxide levels were evaluated using Muse ® Cell Analyzer and Muse ® Oxidative Stress Kit (Luminex Corporation, Austin, TX, USA) as previously described [ 19 ]. Briefly, superoxide-positive and superoxide-negative subpopulations were automatically analyzed and representative histograms are shown.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, superoxide-positive and superoxide-negative subpopulations were automatically analyzed and representative histograms are shown. The glutathione redox potential (GSH/GSSG) was assayed using a Premo™ Cellular Redox Sensor (roGFP-Grx1, P36242, Thermo Fisher Scientific, Waltham, MA, USA) as described comprehensively elsewhere [ 19 ]. Briefly, a baculovirus-based transduction (BacMam 2.0 technology) was used to provide a redox-sensitive green fluorescent protein (roGFP) and glutaredoxin 1 (Grx1) chimeric protein into insulinoma cells and glutathione redox potential-based fluorescent signals (relative fluorescent units, RFU) were measured using a Tecan Infinite ® M200 (Tecan Group Ltd., Männedorf, Switzerland) fluorescence mode microplate reader.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

5-Azacytidine Inhibits the Activation of Senescence Program and Promotes Cytotoxic Autophagy during Trdmt1-Mediated Oxidative Stress Response in Insulinoma β-TC-6 Cells

Filip

Lewińska

Adamczyk‐Grochala

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

5-Azacytidine (5-azaC), a methyltransferase inhibitor and anticancer drug, can promote several cellular stress responses such as apoptosis, autophagy, and senescence. The action of 5-azaC is complex and can be modulated by dose, time of treatment, and co-administration with oxidants. Insulinoma is a rare pancreatic neuroendocrine tumor with limited chemotherapeutic options. In the present study, two cellular models of insulinoma were considered, namely NIT-1 and β-TC-6 mouse cells, to evaluate the effects of 5-azaC post-treatment during hydrogen peroxide-induced oxidative stress. 5-azaC attenuated the development of oxidant-induced senescent phenotype in both cell lines. No pro-apoptotic action of 5-azaC was observed in cells treated with the oxidant. On the contrary, 5-azaC stimulated an autophagic response, as demonstrated by the increase in phosphorylated eIF2α and elevated pools of autophagic marker LC3B in oxidant-treated β-TC-6 cells. Notably, autophagy resulted in increased necrotic cell death in β-TC-6 cells with higher levels of nitric oxide compared to less affected NIT-1 cells. In addition, 5-azaC increased levels of RNA methyltransferase Trdmt1, but lowered 5-mC and m6A levels, suggesting Trdmt1 inhibition. We postulate that the 5-azaC anticancer action may be potentiated during oxidative stress conditions that can be used to sensitize cancer cells, at least insulinoma cells, with limited drug responsiveness.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

5-Azacytidine Inhibits the Activation of Senescence Program and Promotes Cytotoxic Autophagy during Trdmt1-Mediated Oxidative Stress Response in Insulinoma β-TC-6 Cells

Filip

Lewińska

Adamczyk‐Grochala

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

show abstract

“…Taking into account the above, the IFC method seems to be a very good solution, which, unlike FC, has a good real-time control system of the analysed results, thanks to the possibility of digital image recording of cells flowing through the measuring capillary [ 37 , 38 ]. IFC was previously widely used in science for analysis in the biology of human cells [ 42 ], yeast [ 43 ], algae [ 44 ] and bacteria [ 24 ]. Moreover, recently this technique, in combination with the deep learning algorithm, was successfully applied in the identification of pollen grains belonging to families: Lamiaceae , Orobanchaceae , Rubiaceae , Gentianaceae , Asteraceae , Campanulaceae , Apiaceae , Dipsaceae , Primulaceae , Caryophyllaceae , Fabaceae , Rosaceae , Violaceae , Clusiaceae , Parnassiaceae , Cistaceae and Ranunculaceae [ 6 ].…”

Section: Resultsmentioning

confidence: 99%

Imaging Flow Cytometry as a Quick and Effective Identification Technique of Pollen Grains from Betulaceae, Oleaceae, Urticaceae and Asteraceae

Gierlicka

Kasprzyk

Wnuk

2022

Cells

Self Cite

View full text Add to dashboard Cite

Despite the continuous and intensive development of laboratory techniques, a light microscope is still the most common tool used in pollen grains differentiation. However, microscopy is time-consuming and needs well-educated and experienced researchers. Other currently used techniques can be categorised as images and non-images analysis, but each has certain limitations. We propose a new approach to differentiate pollen grains using the Imaging Flow Cytometry (IFC) technique. It allows for high-throughput fluorescence data recording, which, in contrast to the standard FC, also enables real-time control of the results thanks to the possibility of digital image recording of cells flowing through the measuring capillary. The developed method allows us to determine the characteristics of the pollen grains population based on the obtained fluorescence data, using various combinations of parameters available in the IDEAS software, which can be analysed on different fluorescence channels. On this basis, we distinguished pollen grains both between and within different genera belonging to the Betulaceae, Oleaceae, Urticaceae and Asteraceae families. Thereby, we prove that the proposed methodology is sufficient for accurate, fast, and cost-effective identification and potentially can be used in the routine analysis of allergenic pollen grains.

show abstract

“…Doping with silver and copper ions led to two-photon absorption cross sections of up to 5.4410 6 GM and 1.3310 7 GM, respectively, and thus appears to be a method for enhancement of nonlinear optical properties of semiconductor NPLs without increasing their volume [6]. The features of these semiconductor optically active nanosystems relevant for multi-photon fluorescence microscopy applications will be briefly discussed [7].…”

Section: Extended Abstractmentioning

confidence: 99%

Spectrally Resolved Nonlinear Optical Properties Of Colloidal Quantum Confined Semiconductor Dots, Rods And Nanoplatelets

Nyk¹,

Antoniak²,

Nawrot³

et al. 2022

Proceedings of the 8th World Congress on New Technologies

Self Cite

View full text Add to dashboard Cite

Multimodal polymer encapsulated CdSe/Fe3O4 nanoplatform with improved biocompatibility for two-photon and temperature stimulated bioapplications

Cited by 26 publications

References 77 publications

5-Azacytidine Inhibits the Activation of Senescence Program and Promotes Cytotoxic Autophagy during Trdmt1-Mediated Oxidative Stress Response in Insulinoma β-TC-6 Cells

5-Azacytidine Inhibits the Activation of Senescence Program and Promotes Cytotoxic Autophagy during Trdmt1-Mediated Oxidative Stress Response in Insulinoma β-TC-6 Cells

Imaging Flow Cytometry as a Quick and Effective Identification Technique of Pollen Grains from Betulaceae, Oleaceae, Urticaceae and Asteraceae

Spectrally Resolved Nonlinear Optical Properties Of Colloidal Quantum Confined Semiconductor Dots, Rods And Nanoplatelets

Contact Info

Product

Resources

About