Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution

Abstract

We developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray’s superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.

Hot‐Electron‐Mediated Photochemical Reactions: Principles, Recent Advances, and Challenges

“…Since 2010, various experimental and theoretical results have supported the production of DMAB from pATP by plasmon-driven surface-catalyzed chemical reactions. [61][62][63][64][65][66][67][68][69][70][71][72] Adv. Optical Mater.…”

Nanoscale measurement of trace element distributions in Spartina alterniflora root tissue during dormancy

“…The HXN beamline provides a scanning X-ray microscopy capability with multimodality imaging including fluorescence and differential phase contrast2748 at a 15 nm spatial resolution. In this study, the nano-XRF measurement was carried out at 12 keV using a HXN X-ray microscope24 equipped with a pair of multilayer Laue lenses.…”

“…For example, a multilayer Laue lens (MLL)-based scanning hard-X-ray microscope24 has been developed at the Hard X-ray Nanoprobe (HXN) beamline at National Synchrotron Light Source II (NSLS-II). This beamline can provide an ultra-high spatial resolution measurement down to about a 10 nm level for various scientific applications252627. The unique feature of this high detection sensitivity and resolution measurement is to make each pixel size small enough to focus on a specific small area in the root tissue during the measurement.…”

Nm-scale spatial resolution X-ray imaging with MLL nanofocusing optics: Instrumentational requirements and challenges

“…For 3D tomography measurements, an additional rotational degree of freedom is needed. Table 1 Prior to construction, rigorous R&D work has been performed for concept validation and optimization of various components [6,[9][10][11][12]. The microscope relies on piezo driven actuators to minimize heat dissipation and at the same time preserve travel ranges and adequate resolution.…”

“…Different nanofocusing optics can be utilized to achieve high spatial resolution in the hard x-ray regime e.g. Kirkpatrick-Baez (KB) mirrors [5], Fresnel Zone Plates [6,7] and Multilayer Laue Lenses [8][9][10][11] The Hard X-ray Nanoprobe (HXN) beamline at NSLS-II has been designed and constructed to enable scanning imaging experiments with unprecedented spatial resolution and detection sensitivity. We utilized MLL optics to achieve sub-20 nm focused spot size preserving focusing efficiency in the energy range between 6 and 25 keV.…”