Multimode Light Microscopy and the Dynamics of Molecules, Cells, and Tissues

Farkas, Daniel L.; Baxter, GF; DeBiasio, Richard; Gough, Albert; Nederlof, Michel; Pane, D.; Pane, John F.; Patek, David R.; Ryan, K.; Taylor, D. Lansing

doi:10.1146/annurev.ph.55.030193.004033

Cited by 67 publications

(36 citation statements)

References 80 publications

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“…Although the concentration of dye we used in this study would allow monitoring of several divisions, we did not attempt to quantify cellular proliferation. Additional possible causes of a decrease in fluorescence intensity include submersion of the transplanted cells under host BM stroma [5], limited lifetime of mature donor cells in host BM (experiments under way), and photobleaching of the fluorochromes by repeated illumination [41].…”

Section: Discussionmentioning

confidence: 99%

Transplanted Hematopoietic Cells Seed in Clusters in Recipient Bone Marrow In Vivo

et al. 2002

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Section: Discussionmentioning

confidence: 99%

Transplanted Hematopoietic Cells Seed in Clusters in Recipient Bone Marrow In Vivo

et al. 2002

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“…This improves contrast in IRM images as a result of the increased index differential between the substratum and the medium. DIC and IRM images were acquired on a multimode microscope based on a Zeiss (Thornwood, NY) Axiovert microscope that had been automated to allow changes in optical configuration (optical path, DIC analyzer, color and neutral density filters, camera and lamp shutters, stage position, and focus) within a period of a few seconds under software control (STC-View, the multimode microscopy imaging system developed for the Science and Technology Center at Carnegie Mellon University), with simultaneous control of image acquisition and storage into computer memory (Macintosh Quadra 950; Apple, Cupertino, CA) and onto disk Farkas et al, 1993). Images were usually acquired by a video camera (C-2400 Newvicon, Hamamatsu, Bridgewater, NJ) and stored directly into computer memory or onto videotape.…”

Section: Data Acquisitionmentioning

confidence: 99%

Keratocytes Generate Traction Forces in Two Phases

Burton

Park

Taylor

1999

MBoC

172

131

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Forces generated by goldfish keratocytes and Swiss 3T3 fibroblasts have been measured with nanonewton precision and submicrometer spatial resolution. Differential interference contrast microscopy was used to visualize deformations produced by traction forces in elastic substrata, and interference reflection microscopy revealed sites of cell-substratum adhesions. Force ranged from a few nanonewtons at submicrometer spots under the lamellipodium to several hundred nanonewtons under the cell body. As cells moved forward, centripetal forces were applied by lamellipodia at sites that remained stationary on the substratum. Force increased and abruptly became lateral at the boundary of the lamellipodium and the cell body. When the cell retracted at its posterior margin, cell-substratum contact area decreased more rapidly than force, so that stress (force divided by area) increased as the cell pulled away. An increase in lateral force was associated with widening of the cell body. These mechanical data suggest an integrated, two-phase mechanism of cell motility: (1) low forces in the lamellipodium are applied in the direction of cortical flow and cause the cell body to be pulled forward; and (2) a component of force at the flanks pulls the rear margins forward toward the advancing cell body, whereas a large lateral component contributes to detachment of adhesions without greatly perturbing forward movement.

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“…Their alignment was often not repeatable each time a particular filter was rotated into place. Taylor and colleagues have had better repeatable position accuracy using a linear filter slider (Farkas et al, 1993;Taylor et al, 1992); however, this does not eliminate the problem of image registration on the detector.…”

Section: Filter Cube Turret-thementioning

confidence: 99%