Multiparametric magnetic immunoassays utilizing non-linear signatures of magnetic labels

Lenglet, Luc

doi:10.1016/j.jmmm.2009.02.104

Cited by 27 publications

(16 citation statements)

References 3 publications

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“…A study has looked into simultaneous quantification of multiple mNPs [37] in which the authors and were able to accurately quantify three mNPs using mNP saturation harmonics. Another group has attempted to distinguish nanobeads [38, 39] where the authors quantified the derivatives of the n th order magnetic induction field with respect to the magnetic field in order to create magnetic signatures for different nanobeads. The authors were then able to quantify mixtures of nanobeads.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic AC susceptibility imaging (sASI) of magnetic nanoparticles

Ficko

Nadar

Diamond

2015

Journal of Magnetism and Magnetic Materials

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This study demonstrates a method for alternating current (AC) susceptibility imaging (ASI) of magnetic nanoparticles (mNPs) using low cost instrumentation. The ASI method uses AC magnetic susceptibility measurement to create tomographic images using an array of drive coils, compensation coils and fluxgate magnetometers. Using a spectroscopic approach in conjunction with ASI, a series of tomographic images can be created for each frequency measurement and is termed sASI. The advantage of sASI is that mNPs can be simultaneously characterized and imaged in a biological medium. System calibration was performed by fitting the in-phase and out-of-phase susceptibility measurements of an mNP sample with a hydrodynamic diameter of 100 nm to a Brownian relaxation model (R2 = 0.96). Samples of mNPs with core diameters of 10 and 40 nm and a sample of 100 nm hydrodynamic diameter were prepared in 0.5 ml tubes. Three mNP samples were arranged in a randomized array and then scanned using sASI with six frequencies between 425 and 925 Hz. The sASI scans showed the location and quantity of the mNP samples (R2 = 0.97). Biological compatibility of the sASI method was demonstrated by scanning mNPs that were injected into a pork sausage. The mNP response in the biological medium was found to correlate with a calibration sample (R2 = 0.97, p <0.001). These results demonstrate the concept of ASI and advantages of sASI.

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Section: Introductionmentioning

confidence: 99%

Spectroscopic AC susceptibility imaging (sASI) of magnetic nanoparticles

Ficko

Nadar

Diamond

2015

Journal of Magnetism and Magnetic Materials

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“…The MIAplex ® sensor measures a signal, which is proportional to the second derivative of a magnetization curve [11]. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Such applications of these single nanoparticles are unexplored and are preliminary demonstrated through the MIAplex ® measurements for multiparametric tests [11].…”

Section: Introductionmentioning

confidence: 99%

An innovative nanoprobe for magnetic immunoassay: Individual γ-Fe2O3 nanoparticles; towards high sensitive and multiparametric detection

Montferrand¹,

Jouni²,

Lièvre³

et al. 2011

IRBM

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“…Osterfeld et al (2008) used an array of giant magnetoresistive sensors, each tagged with an antibody for a specific analyte. Lenglet (2009) used the unique magnetic response of two different micron size particles to quantify each particle for immunoassays without the need for spatial resolution. However, micron-sized particles have limited in vivo applications because their large size limits the distribution.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of multiple magnetic nanoparticles

2010

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Distinct magnetic nanoparticle designs can have unique spectral responses to an AC magnetic field in a technique called the magnetic spectroscopy of Brownian motion (MSB). The spectra of the particles have been measured using desktop spectrometers and in vivo measurements. If multiple particle types are present in a region of interest, the unique spectral signatures allow for the simultaneous quantification of the various particles. We demonstrate such a potential experimentally with up to three particle types. This ability to concurrently detect multiple particles will enable new biomedical applications.

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Multiparametric magnetic immunoassays utilizing non-linear signatures of magnetic labels

Cited by 27 publications

References 3 publications

Spectroscopic AC susceptibility imaging (sASI) of magnetic nanoparticles

Spectroscopic AC susceptibility imaging (sASI) of magnetic nanoparticles

An innovative nanoprobe for magnetic immunoassay: Individual γ-Fe2O3 nanoparticles; towards high sensitive and multiparametric detection

Simultaneous quantification of multiple magnetic nanoparticles

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