Simultaneous quantification of multiple magnetic nanoparticles

Rauwerdink, Adam M.; Giustini, Andrew J.; Weaver, John B.

doi:10.1088/0957-4484/21/45/455101

Cited by 38 publications

(26 citation statements)

References 22 publications

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“…Larger mNPs that relax via Brownian mechanisms are used to maximize the number of environmental factors that influence the spectra. The approach allows facile measurement of temperature [13–15], viscosity [16,17], relaxation time [18], mNP uptake in cells [19], matrix stiffness [20], bound state [21–25] and mNP number [26]. Tumor microenvironment characteristics can be measured using the shape of the spectra, because it reflects the ability of the mNPs to align with the alternating field.…”

Section: Project 2: Spectroscopic Quantification Of Ligand Binding Inmentioning

confidence: 99%

The Dartmouth Center for Cancer Nanotechnology Excellence: Magnetic Hyperthermia

Baker

Fiering

Griswold³

et al. 2015

Nanomedicine (Lond.)

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The Dartmouth Center for Cancer Nanotechnology Excellence – one of nine funded by the National Cancer Institute as part of the Alliance for Nanotechnology in Cancer – focuses on the use of magnetic nanoparticles for cancer diagnostics and hyperthermia therapy. It brings together a diverse team of engineers and biomedical researchers with expertise in nanomaterials, molecular targeting, advanced biomedical imaging and translational in vivo studies. The goal of successfully treating cancer is being approached by developing nanoparticles, conjugating them with Fabs, hyperthermia treatment, immunotherapy and sensing treatment response.

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Section: Project 2: Spectroscopic Quantification Of Ligand Binding Inmentioning

confidence: 99%

The Dartmouth Center for Cancer Nanotechnology Excellence: Magnetic Hyperthermia

Baker

Fiering

Griswold³

et al. 2015

Nanomedicine (Lond.)

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“…(Rauwerdink et al 2010; Rauwerdink and Weaver 2010b; Weaver and Kuehlert 2012; Weaver et al 2009) Particularly of interest to biosensing applications, MSB is able to quantitatively measure the bound fraction (Rauwerdink and Weaver 2011) and relaxation times (Weaver and Kuehlert 2012). The measurements rely on the fact that mNPs tend to align with the applied magnetic field, but that tendency is countered by Brownian motion that randomizes the mNPs’ alignment.…”

Section: Introductionmentioning

confidence: 99%

Molecular sensing with magnetic nanoparticles using magnetic spectroscopy of nanoparticle Brownian motion

Zhang

Reeves

Perreard

et al. 2013

Biosensors and Bioelectronics

102

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Functionalized magnetic nanoparticles (mNPs) have shown promise in biosensing and other biomedical applications. Here we use functionalized mNPs to develop a highly sensitive, versatile sensing strategy required in practical biological assays and potentially in vivo analysis. We demonstrate a new sensing scheme based on magnetic spectroscopy of nanoparticle Brownian motion (MSB) to quantitatively detect molecular target. MSB uses the harmonics of oscillating mNPs as a metric for the freedom of rotational motion, thus reflecting the bound state of the mNP. The harmonics can be detected in vivo from nanogram quantities of iron within 5 seconds. Using a streptavidin-biotin binding system, we show that the detection limit of the current MSB technique is lower than 150 pM (0.075 picomole), which is much more sensitive than previously reported techniques based on mNP detection. Using mNPs conjugated with two anti-thrombin DNA aptamers, we show that thrombin can be detected with high sensitivity (4 nM or 2 picomole). A DNA-DNA interaction was also investigated. The results demonstrated that sequence selective DNA detection can be achieved with 100 pM (2 nM) sensitivity. The results of using MSB to sense these interactions, verified that the MSB based sensing technique can achieve rapid measurement (within 10 sec), and is suitable for detecting and quantifying a wide range of biomarkers or analytes. It has the potential to be applied in variety of biomedical applications or diagnostic analyses.

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“…AC rotational magnetic fields have been exploited to enhance the measurement of the out-of-phase susceptibility component of mNPs [19, 35, 36]. A study has looked into simultaneous quantification of multiple mNPs [37] in which the authors and were able to accurately quantify three mNPs using mNP saturation harmonics. Another group has attempted to distinguish nanobeads [38, 39] where the authors quantified the derivatives of the n th order magnetic induction field with respect to the magnetic field in order to create magnetic signatures for different nanobeads.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic AC susceptibility imaging (sASI) of magnetic nanoparticles

Ficko

Nadar

Diamond

2015

Journal of Magnetism and Magnetic Materials

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This study demonstrates a method for alternating current (AC) susceptibility imaging (ASI) of magnetic nanoparticles (mNPs) using low cost instrumentation. The ASI method uses AC magnetic susceptibility measurement to create tomographic images using an array of drive coils, compensation coils and fluxgate magnetometers. Using a spectroscopic approach in conjunction with ASI, a series of tomographic images can be created for each frequency measurement and is termed sASI. The advantage of sASI is that mNPs can be simultaneously characterized and imaged in a biological medium. System calibration was performed by fitting the in-phase and out-of-phase susceptibility measurements of an mNP sample with a hydrodynamic diameter of 100 nm to a Brownian relaxation model (R2 = 0.96). Samples of mNPs with core diameters of 10 and 40 nm and a sample of 100 nm hydrodynamic diameter were prepared in 0.5 ml tubes. Three mNP samples were arranged in a randomized array and then scanned using sASI with six frequencies between 425 and 925 Hz. The sASI scans showed the location and quantity of the mNP samples (R2 = 0.97). Biological compatibility of the sASI method was demonstrated by scanning mNPs that were injected into a pork sausage. The mNP response in the biological medium was found to correlate with a calibration sample (R2 = 0.97, p <0.001). These results demonstrate the concept of ASI and advantages of sASI.

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Simultaneous quantification of multiple magnetic nanoparticles

Cited by 38 publications

References 22 publications

The Dartmouth Center for Cancer Nanotechnology Excellence: Magnetic Hyperthermia

The Dartmouth Center for Cancer Nanotechnology Excellence: Magnetic Hyperthermia

Molecular sensing with magnetic nanoparticles using magnetic spectroscopy of nanoparticle Brownian motion

Spectroscopic AC susceptibility imaging (sASI) of magnetic nanoparticles

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