Multiparity Differentially Affects Specific Aspects of the Acute Neuroinflammatory Response to Traumatic Brain Injury in Female Mice

Gilfarb, Rachel A.; Tapp, Zoe; Lemanski, Elise; Velasquez, John; Cornelius, Sydney; Kokiko-Cochran, Olga N.; Leuner, Benedetta

doi:10.1016/j.neuroscience.2022.12.011

Cited by 1 publication

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 77 , 78 We previously reported that TBI and sleep disruption each decrease AQP4 polarization acutely after injury. 39 , 47 Here, we show that AQP4 expression is differentially influenced by TBI with SF-R. AQP4 expression was enhanced with TBI alone whereas TBI SF-R had the lowest expression of perivascular AQP4. Further, this perivascular AQP4 expression had a sex-dependent response, with the TBI-induced enhancements of perivascular AQP4 being driven by males.…”

Section: Discussionmentioning

confidence: 62%

“…AQP4 intensity and polarization were determined using custom in-house software as previously described. 39 , 47 AQP4 polarization was calculated as the ratio of perivascular to parenchymal (20 μm from vessel) AQP4 immunofluorescence. Representative images for AQP4 were cropped from the original analysis image to emphasize the large vessel used in analysis.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Divergent Spatial Learning, Enhanced Neuronal Transcription, and Blood–Brain Barrier Disruption Develop During Recovery from Post-Injury Sleep Fragmentation

Tapp,

Ren,

Palmer

et al. 2023

Neurotrauma Reports

Self Cite

View full text Add to dashboard Cite

Traumatic brain injury (TBI) causes pathophysiology that may significantly decrease quality of life over time. A major propagator of this response is chronic, maladaptive neuroinflammation, which can be exacerbated by stressors such as sleep fragmentation (SF). This study determined whether post-TBI SF had lasting behavioral and inflammatory effects even with a period of recovery. To test this, male and female mice received a moderate lateral fluid percussion TBI or sham surgery. Half the mice were left undisturbed, and half were exposed to daily SF for 30 days. All mice were then undisturbed between 30 and 60 days post-injury (DPI), allowing mice to recover from SF (SF-R). SF-R did not impair global Barnes maze performance. Nonetheless, TBI SF-R mice displayed retrogression in latency to reach the goal box within testing days. These nuanced behavioral changes in TBI SF-R mice were associated with enhanced expression of neuronal processing/signaling genes and indicators of blood–brain barrier (BBB) dysfunction. Aquaporin-4 (AQP4) expression, a marker of BBB integrity, was differentially altered by TBI and TBI SF-R. For example, TBI enhanced cortical AQP4 whereas TBI SF-R mice had the lowest cortical expression of perivascular AQP4, dysregulated AQP4 polarization, and the highest number of CD45 + cells in the ipsilateral cortex. Altogether, post-TBI SF caused lasting, divergent behavioral responses associated with enhanced expression of neuronal transcription and BBB disruption even after a period of recovery from SF. Understanding lasting impacts from post-TBI stressors can better inform both acute and chronic post-injury care to improve long-term outcome post-TBI.

show abstract