Multipartite nature of potato virus X

Price, M

doi:10.1128/jvi.67.1.596-600.1993

Cited by 7 publications

(5 citation statements)

References 34 publications

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“…Comparison of PMV, PVX and other potexvirus 5′‐NTR sequences indicates repeated AC‐rich elements within regions predicted to be unstructured (Lok and AbouHaidar, 1986; Miller et al ., 1999; Sit et al ., 1994). Although such sequences in the 5′‐NTR of PVX RNA may direct assembly, it is also possible that elements in the 3′‐region may be required, because there is one report indicating that PVX sgRNAs are packaged (Price, 1993). In addition, sgRNA packaging has been noted for several other potexviruses (Bendena et al ., 1987; Forster et al ., 1987; Short and Davies, 1983; White and Mackie, 1990).…”

Section: Requirements For Cis‐acting Rna Elementsmentioning

confidence: 99%

Potato virus X: a model system for virus replication, movement and gene expression

Batten

Yoshinari

Hemenway

2003

Molecular Plant Pathology

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SUMMARY Considerable research has focused on the cis‐ and trans‐acting components required for various aspects of the potato virus X (PVX) infection process. In addition, the development of PVX‐based vectors has facilitated analyses of the PVX infection process and provided diverse technological applications. As a result, the PVX system will continue to serve as a model for analyses of processes such as virus movement, RNA replication, and gene silencing, and as a tool for protein expression.

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Section: Requirements For Cis‐acting Rna Elementsmentioning

confidence: 99%

Potato virus X: a model system for virus replication, movement and gene expression

Batten

Yoshinari

Hemenway

2003

Molecular Plant Pathology

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“…In TEM TMGMV labeled via N ‐terminus measured 175±23 nm, via tyrosine residues 152±41 nm and via glutamic acid residues 177±17 nm (Figure 3C), which are shorter than expected. While TMGMV forms 300 nm nucleoprotein complexes, the TMGMV preparations show shorter rods, even in untreated samples (191.4±21 nm, Figure S4) – this could be due to grid preparation or sample processing [63] …”

Section: Resultsmentioning

confidence: 99%

“…While TMGMV forms 300 nm nucleoprotein complexes, the TMGMV preparations show shorter rods, even in untreated samples (191.4 � 21 nm, Figure S4)this could be due to grid preparation or sample processing. [63] Next, addressability of the reactive sites using biotin tags was assessed. Biotin is a small vitamin that is easily detected through interaction with avidin/streptavidin conjugates [64] in biochemical assays such as Western blot.…”

Section: Reactivity Of Tmgmv and Analysis Of Vnpsmentioning

confidence: 99%

Bioconjugation Strategies for Tobacco Mild Green Mosaic Virus

et al. 2022

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Tobacco mild green mosaic virus (TMGMV) is a plant virus closely related to Tobacco mosaic virus (TMV), sharing many of its structural and chemical features. These rod-shaped viruses, comprised of 2130 identical coat protein subunits, have been utilized as nanotechnological platforms for a myriad of applications, ranging from drug delivery to precision agriculture. This versatility for functionalization is due to their chemically active external and internal surfaces. While both viruses are similar, they do exhibit some key differences in their surface chemistry, suggesting the reactive residue distribution on TMGMV should not overlap with TMV. In this work, we focused on the establishment and refinement of chemical bioconjugation strategies to load molecules into or onto TMGMV for targeted delivery. A combination of NHS, EDC, and diazo coupling reactions in combination with click chemistry were used to modify the N-terminus, glutamic/aspartic acid residues, and tyrosines in TMGMV. We report loading with over 600 moieties per TMGMV via diazo-coupling, which is a > 3-fold increase compared to previous studies. We also report that cargo can be loaded to the solvent-exposed N-terminus and carboxylates on the exterior/interior surfaces. Mass spectrometry revealed the most reactive sites to be Y12 and Y72, both tyrosine side chains are located on the exterior surface. For the carboxylates, interior E106 (66.53 %) was the most reactive for EDC-propargylamine coupled reactions, with the exterior E145 accounting for > 15 % reactivity, overturning previous assumptions that only interior glutamic acid residues are accessible. A deeper understanding of the chemical properties of TMGMV further enables its functionalization and use as a multifunctional nanocarrier platform for applications in medicine and precision farming.

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“…Potato virus X (PVX) is the type member of the genus potexvirus and has a monopartite genome composed of a single, positive strand 6.4 kb genomic RNA (gRNA) encoding five open reading J o u r n a l P r e -p r o o f 5 frames (ORFs). The replication of PVX consists in the synthesis of both strands of gRNA and subgenomic RNAs (Price, 1992), and the positive-strand RNAs have a 5´ Cap (Sonenberg et al, 1978) and a 3' poly-A tail (Price, 1993). In Nicotiana benthamiana, PVX produces local and systemic infection and the typical symptoms are concentric chlorosis, vascular clearing or mottled chlorosis.…”

Section: Introductionmentioning

confidence: 99%