Multiphasic electrophoresis with diversified spacers

Abstract

The principle and some applications of multiphasic electrophoresis with diversified spacers are described. The method combines previously published concepts of introducing highly diversified spacers into the stack of an isotachophoretic system with the theory of snow- and telescope-electrophoresis. The link between this electrophoretic method and isotachophoresis on the one hand and monophasic zone electrophoresis on the other hand is exemplified. The importance of the nature of the spacers as well as of the pH and ions in the leading and terminating zone is illustrated. Details are given concerning the experimental set-up. This electrophoretic technique could serve as an alternative to isoelectric focusing.

Counterflow Isotachophoresis As a Method of Concentration and Isolation of DNA from Biological Fluids

“…1). There are well-known restrictions of employment of this method (specifically, deformation of gel in nonuniform electric field, which occurs in the course of counterflow isotachophoresis and limits its duration) [4,14]. Indeed, in long-term process, gel is deformed, and the zone of separation is distorted (data not shown).…”

Concentration and Isolation of DNA from Biological Fluids by Agarose Gel Isotachophoresis

“…In the case of low osmotic agarose gel, the counterflow due to electroosmotic force is negligible. The drawbacks of this technique, namely the deformation of the gel in a nonhomogeneous electric field, are well known (20). Indeed, when the process occurs long enough, the gel becomes deformed and the separation area distorted (data not shown).…”

The Use of Poly(2-acrylamido-2-methyl-1-propanesulfonic Acid) Polymers as Spacers for Isotachophoresis in Sieving Gel Matrices

“…To quote from Bringard and Charlionet (14): "Concerning the nature of the spacers, we have shown that Ampholine 5-7 or Pharmalyte 6.7-7.7 are not ideal, because their electrophoretic mobility depends on pH. Highly diversified spacers of nonamphoteric nature and with adequate mobilities for the analysis of proteins would certainly be welcome."…”