Multiple antibiotic resistance and virulence factors of Staphylococcus aureus strains isolated from dairy farms in South Sulawesi, Indonesia

Juwita, Sartika; Indrawati, Agustin; Soejoedono, Retno Damajanti; Safika, Safika; Mayasari, Ni Luh Putu Ika

doi:10.13057/biodiv/d230244

Cited by 9 publications

(13 citation statements)

References 55 publications

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“…Several studies have reported the production of hemolysins by S. aureus strains isolated from food sources. In agreement with the results of this study, a study by Juwita et al (2022) investigated the hemolysin production in S. aureus strains isolated from dairy products and human, and found that a signi cant proportion of the isolates produced hemolysin. The production of hemolysins by S. aureus strains of food origin has been associated with their ability to cause foodborne illnesses.…”

Section: Discussionsupporting

confidence: 89%

Molecular detection of Alpha toxin in local Staphylococcus aureus isolates

Alkhafaji¹,

Musafer

Faqri³

et al. 2023

Preprint

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Bacterial toxins are the most important virulence factors that could hurt humans causing serious health problems. Staphylococcus aureus have a wide range of extracellular toxins; one of them is alpha toxin; haemolysin that degrade red blood cells. Thirty S. aureus isolates were isolated from clinical and animal-origin foods sample. Staphylococcus aureus isolates were chosen for the phenotypic and genotypic detection of alpha toxin. Bacterial deoxy ribonucleic acid was extracted and specific primers were designated for this study. Polymerase chain reaction was depended to detect the prevalence of haemolysin coding gene in local isolates in Iraq. On the other hand, silver nanoparticles were biosynthesized using food origin bacterial isolate of Salmonella. The biosynthesis was done in optimized conditions, purified, and tested for their antimicrobial activity. Phenotypic detection methods revealed the ability of 70% to produce alpha toxin. Whereas the genetic tool was very precise in detecting the genetic content (93.33%) of alpha toxin gene in clinical and animal-origin foods. The biosynthesized nanoparticles were with an average diameter of 44.89 nm. They have an antimicrobial activity against haemolysin producing S. aureus isolates. Monitoring and understanding the hemolysin production by S. aureus strains in food samples can aid in implementing effective food safety measures to prevent contamination and ensure the safety of food products.

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Section: Discussionsupporting

confidence: 89%

Molecular detection of Alpha toxin in local Staphylococcus aureus isolates

Alkhafaji¹,

Musafer

Faqri³

et al. 2023

Preprint

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“…In South Africa's Kariega estuary, 22 percent of S. aureus tetA genes were identified, similarly to the case with the tetA gene identified in this research [41], S. aureus strains obtained from Indonesian dairy farms in South Sulawesi Province all had the tetA gene [42]. In S. aureus, the tetA gene, in association with tetB, is necessary for the drug efflux pump that provides a significant amount of tetracycline resistance and is carried predominantly on a plasmid [43].…”

Section: Introductionsupporting

confidence: 77%

Identification of Antibiotic Resistance Pattern and Antibiotic Resistance Gene of Staphylococcus aureus from Broiler Chicken Farm in Sukabumi and Cianjur Regency, West Java Province, Indonesia

Hermana

Safika

Indrawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Broiler chicken is one of the most common forms of protein in Indonesia. Sukabumi and Cianjur are two of the province of West Java’s top producers of broiler chickens. Farmers nonetheless use antibiotics for prevention, treatment, and growth enhancement. The objective of this investigation is to detect antibiotic resistance and the related resistance gene. The cloacal swab samples were determined to be Staphylococcus aureus by biochemical and molecular assays. Against the positive S. aureus strains, the antibiotics tetracycline, ampicillin, gentamicin, nalidixic acid, ciprofloxacin, erythromycin, and chloramphenicol were evaluated. The colony’s obvious inhibitory zone was evaluated in accordance with the 2018 Clinical and Laboratory Standard Institute recommendations (CLSI 2018). After extracting the DNA from resistant strains, the polymerase chain reaction (PCR) method was employed to identify the resistance gene. The total number of S. aureus isolates from both regions was 26, with 14 coming from Sukabumi and 12 from Cianjur. The vast majority were resistant to the vast majority of antibiotics used in broiler farms including several medicines. These samples carried the genes blaTEM, gyrA, tetA, and ermB for antibiotic resistance.

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“…Antibiotic resistance pattern results show that all the isolates (100%) exhibited resistance to penicillin, and almost all were resistant to ampicillin (91.42%). is alarming rate of resistance to beta-lactam antibiotics is corroborated by some recent findings [54,55] and can be explained by the common use of β-lactams in the treatment of bovine mastitis [56]. A study carried out in Kenya by Mbindyo et al found that 71.4% of S. aureus strains exhibited resistance to ampicillin [57].…”

Section: Discussionmentioning

confidence: 85%

Relationship between Biofilm Formation and Antibiotic Resistance and Adherence Genes in Staphylococcus aureus Strains Isolated from Raw Cow Milk in Shahrekord, Iran

Pajohesh

Tajbakhsh

Momtaz

et al. 2022

International Journal of Microbiology

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The production of biofilms by S. aureus contributes significantly to treatment failures. The present study aims to establish the relationship between biofilm formation and antibiotic resistance and adhesion genes in Staphylococcus aureus strains isolated from raw cow milk in Shahrekord, Iran. A total of 90 samples of raw cow’s milk were collected. Presumptive S. aureus strains were obtained using Baird-Parker plates after enrichment in tryptone soy broth, and final colonies were selected from brain heart infusion. Additional tests such as coagulase were done, and the identification was confirmed by the detection of the aroA gene. Biofilm producing strains were screened using a spectrophotometry method applied to microplates. Crystal violet staining was used to quantify the formation of biofilm. An antibiotic susceptibility test was performed using the Kirby–Bauer disc diffusion method. PCR was used to detect several biofilm and antibiotics resistance related genes. The chi-square test and Fisher’s exact test were used to establish a statistically significant relationship between biofilm reaction and antibiotic resistance ( p value <0.05). Results show a moderate (38.88%) recovery rate of S. aureus in milk and 65.71% of the isolates were strong biofilm producers. Antibiotic susceptibility tests show an alarming rate of resistance to beta-lactam antibiotics, especially penicillin (100%), ampicillin (91.42%), and oxacillin (71.42%). This finding correlates with antibiotic resistance gene detection, in which the gene blaZ was most found (71.42%), followed by mecA and Aac-D (42.85%). Detection of biofilm-related genes shows that all the genes targeted were found among S. aureus isolates. Statistical tests show a significant correlation between biofilm production and antibiotic resistance in S. aureus. This study revealed that there is a significant correlation between biofilm production and antibiotic resistance in S. aureus isolated from raw milk. These results highlight the need for regular surveillance of the occurrence of S. aureus strains in milk and milk products in Iran.

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Multiple antibiotic resistance and virulence factors of Staphylococcus aureus strains isolated from dairy farms in South Sulawesi, Indonesia

Cited by 9 publications

References 55 publications

Molecular detection of Alpha toxin in local Staphylococcus aureus isolates

Molecular detection of Alpha toxin in local Staphylococcus aureus isolates

Identification of Antibiotic Resistance Pattern and Antibiotic Resistance Gene of Staphylococcus aureus from Broiler Chicken Farm in Sukabumi and Cianjur Regency, West Java Province, Indonesia

Relationship between Biofilm Formation and Antibiotic Resistance and Adherence Genes in Staphylococcus aureus Strains Isolated from Raw Cow Milk in Shahrekord, Iran

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