1999
DOI: 10.1002/(sici)1522-2594(199907)42:1<197::aid-mrm26>3.0.co;2-5
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Multiple bond13C-13C spin-spin coupling provides complementary information in a13C NMR isotopomer analysis of glutamate

Abstract: Most 13 C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13 C isotopomers as reported by one-bond 13 C-13 C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13 C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13 C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in… Show more

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Cited by 17 publications
(13 citation statements)
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“…2a). The ␤ proton resonance of [3][4][5][6][7][8][9][10][11][12][13] C]lactate at 1.30 ppm is a "doublet of doublets" with 1 J ␤H-␤C ϭ 128 Hz and the previously determined 3 J ␣H-␤H (Fig. 2b).…”
Section: Resultsmentioning
confidence: 93%
See 1 more Smart Citation
“…2a). The ␤ proton resonance of [3][4][5][6][7][8][9][10][11][12][13] C]lactate at 1.30 ppm is a "doublet of doublets" with 1 J ␤H-␤C ϭ 128 Hz and the previously determined 3 J ␣H-␤H (Fig. 2b).…”
Section: Resultsmentioning
confidence: 93%
“…13 C]lactate, [1][2][3][4][5][6][7][8][9][10][11][12][13] C]glucose, and [2-13 C]pyruvate we collected the 1 H spectra of effluent samples to assess the contributions of glucose and glycogen to lactate efflux. The effluent contained [3- 13 C]lactate.…”
mentioning
confidence: 99%
“…Second, the multiplet pattern in the glutamate C2 resonance also changes, and the individual lines appear to become broader at the higher glucose concentration. This apparent linebroadening is the result of long-range coupling between glutamates C2 and C5 (24), reflecting an increase in [U- 13 C 5 ]glutamate with increasing glucose concentration. However, the total glutamate concentration, as estimated by NMR areas, did not increase with increasing glucose concentration, in opposition to one recent report (25), but in agreement with another (26).…”
Section: Methodsmentioning
confidence: 98%
“…Therefore Glu-C3D actually was the sum of Glu-C3D32 and Glu-C3D34. Note that a given multiplet can represent the sum of different 13 44 ) compared with the linewidth of 13 C resonances observed in vivo, and their effect on linewidth and lineshape was assessed to be negligible in this study.…”
Section: Notationmentioning
confidence: 97%