Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.

Brindle, Paul K.; Holland, J P; Willett, C E; Innis, M A; Holland, Michael J.

doi:10.1128/mcb.10.9.4872

Cited by 90 publications

(88 citation statements)

References 26 publications

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“…Between the first and the second putative CT-boxes, two overlapping putative binding sites for Rap1p (RPG-box, RTR-CACCCANNCMCC, in which M = A/C; Graham and Chambers, 1994) (Chambers et al, 1995). Furthermore, two putative binding sites (11/13 and 9/13 matches) for Abf1p (RTCRYNNNNNACG; Einerhand et al, 1995), which is also involved in the regulation of ENO2 and PGK1 expression (Brindle et al, 1990;Chambers et al, 1990;Packham et al, 1996), were found between CT-box2 and CT-box3. These observations suggested the possibility that this region is the regulatory region of GCR1, and that GCR1 is regulated in the same manner as glycolytic genes.…”

Section: Uas Region Of Gcr1 Contains the Ct-boxesmentioning

confidence: 99%

Expression of GCR1, the transcriptional activator of glycolytic enzyme genes in the yeast Saccharomyces cerevisiae, is positively autoregulated by Gcr1p

Sasaki

Kishimoto

Mizuno

et al. 2005

Yeast

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When regulation of GCR1 expression was analysed using a GCR1-lacZ fusion, lacZ expression levels were decreased in the gcr1 or gcr2 mutant. RT-PCR analysis of genomic GCR1 transcript confirmed the dependency of GCR1 expression on the Gcr1p-Gcr2p complex. Examination of the 5 non-coding region of GCR1 identified three putative Gcr1p binding sites (CT-boxes) in the −100 to −200 region of GCR1, and the putative binding sites for Rap1p (RPG-box) and Abf1p were also identified nearby. The region containing putative cis-elements was analysed by cloning it upstream of the CYC 1 TATA -lacZ fusion. The GCR1 UAS -CYC 1 TATA -lacZ fusion showed a moderate activity and, as expected, the activity was drastically reduced in the gcr1 or gcr2 mutant. Systematic deletion and mutation analyses of ciselements in this region demonstrated that the putative binding sites for Rap1p and Abf1p were not involved in the promoter activity of GCR1 UAS and only one of the three CT-boxes showed GCR1 -and GCR2 -dependent promoter activity. In contrast to the expression of glycolytic genes, where a RPG-box adjacent to the CT-box is required for strong promoter activities, CT-box-dependent expression of GCR1 did not require the RPG-box. Also, a contribution of Sgc1p, an E-box binding transcription factor, to the expression of GCR1 was suggested, based on its disruption analysis.

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Section: Uas Region Of Gcr1 Contains the Ct-boxesmentioning

confidence: 99%

Expression of GCR1, the transcriptional activator of glycolytic enzyme genes in the yeast Saccharomyces cerevisiae, is positively autoregulated by Gcr1p

Sasaki

Kishimoto

Mizuno

et al. 2005

Yeast

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“…Strain MEW85 serA was infected with A placMu9 as described above and incubated in minimal glucose-kanamycin medium with glycine; ampicillin was added after 60 min. After about 4 h, the cells were washed and spread on minimal lactose-kanamycin plates with L-serine. Colonies that grew with glucose and L-serine but not with glucose and glycine were retained as possible glycine cleavage mutants (18).…”

Section: Methodsmentioning

confidence: 99%

Lambda placMu insertions in genes of the leucine regulon: extension of the regulon to genes not regulated by leucine

1992

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The leucine regulon coordinates the expression of several Escherichia coli genes according to the presence of exogenous leucine, which interacts with the bp gene product, Lrp. We isolated and characterized 22 strains with k placMu insertions in Lrp-regulated genes. Lrp and leucine influenced gene expression in a surprising variety of ways. We identified two genes that are regulated by Lrp and not affected by L-leucine. We therefore rename this the leucine-fp regulon. Genes coding for glycine cleavage and leucine biosynthesis enzymes have been identified as members of the leucine4rp regulon. We suggest that the brp gene product activates genes needed for growth in minimal medium, and we show that the gene is repressed by its own product and is highly repressed during growth in rich medium.We recently showed that in Escherichia coli the branchedchain amino acid L-leucine is the effector of a global response that governs a group of genes known as the leucine regulon (11). Regulation of the synthesis of a number of enzymes from different metabolic pathways is affected by the presence of L-leucine in the growth medium (11,16). This response to exogenous L-leucine was altered in an E. coli mutant carrying an insertion in a gene at 20 min (11). This gene, referred to originally as rbl, was renamed lip by consensus among interested investigators (11,20).The lip gene codes for a 38-kDa dimer, the leucineresponsive regulatory protein (Lrp), which has been purified extensively (31). Under the name of the ilvIH binding protein, it was shown to bind to two sites upstream of ilvIH (22), for which it is a positive regulator. A mutation in lrp made the strain constitutive for ilvIH expression (20) and changed only one nucleotide, resulting in a substitution of Glu for Asp. The lip gene was shown to be identical to oppI (2) and is also thought to be identical to livR (12,20).Until now, the genes of the leucine regulon have been identified by guesswork or by the accident of a previously known regulatory gene proving to be identical to lip. As a result of a somewhat different accident, lysU has now been added to the regulon. We present here the results of a screening among random X placMu9 insertions for leucineand Lrp-regulated expression of lacZ. We used a system based on that used for the isolation of din genes (8). We also show that some genes are regulated by Lrp without leucine, and therefore we rename the regulon as the leucine-Lrp regulon. MATERIALS AND METHODSCultures. The strains used in this study-all derivatives of E. coli K-12-are described in Table 1. The plasmids used are also listed in Table 1. Cultures were grown as described previously (11) with L-isoleucine and L-valine (each at 50 ,g/ml) added to the minimal medium used to grow strain CU1008 and all its derivatives to compensate for the ilvA mutation carried by these strains. Other genetic techniques. Plasmid isolations, DNA manipulations, transductions, and transformations were performed as described previously (13, 14).Enzyme assays. L-Serine deaminase was as...

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“…The RAP1 binding site at the HMR silent mating-type locus is required for full repression of transcription [6][7][8][9][10]. RAPI binding sites are also found upstream of a large number of genes including ribosomal and glycolytic enzyme genes activating gene transcription [11][12][13][14]. RAP1 also binds to ARSs (autonomously replicating sequences) and telomeres of yeast chromosomes to regulate cell division [10,15].…”

Section: Introductionmentioning

confidence: 99%

Activation of gastrin gene transcription in islet cells by a RAP1‐like cis‐acting promoter element

1994

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Gastrin transcription in islet cells is activated by a cis-regulatory sequence containing a binding site for the yeast transcription factor RAP1. The DNA-protein interactions between RAP1 protein and the gastrin DNA element determined by methylation interference assays are identical to those of RAP1 and yeast genes. Point mutations in the gastrin RAP1 binding site, which abolished RAP1 binding, decreased transcriptional activation by this sequence. Islet cells revealed a DNA binding protein with RAPl-like binding specificity. These findings support the conclusion that gastrin transcription is activated in mammalian cells by a RAPl-like transcription factor.

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Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.

Cited by 90 publications

References 26 publications

Expression of GCR1, the transcriptional activator of glycolytic enzyme genes in the yeast Saccharomyces cerevisiae, is positively autoregulated by Gcr1p

Expression of GCR1, the transcriptional activator of glycolytic enzyme genes in the yeast Saccharomyces cerevisiae, is positively autoregulated by Gcr1p

Lambda placMu insertions in genes of the leucine regulon: extension of the regulon to genes not regulated by leucine

Activation of gastrin gene transcription in islet cells by a RAP1‐like cis‐acting promoter element

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