Multiple HPV genotypes in cervical carcinomas: improved DNA detection and typing in archival tissues

Huang, Lee-Wen; Chao, Shiouh-Lirng; Chen, Pei-Hsin; Chou, Hui-Ping

doi:10.1016/s1386-6532(03)00167-7

Cited by 55 publications

(52 citation statements)

References 31 publications

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“…In accord with several previous reports from Thailand (Chichareon et al, 1998;Siriaunkgul et al, 2008;Suthipintawong et al, 2011;Chinchai et al, 2012) and Asia (Huang et al, 2004;Bhatla et al, 2008;Chinchai et al, 2012;Kawana et al, 2012), we confirmed that in Northeast Thailand HPV-16 is the most prevalent type of HPV among women with SCCA (56.21%). In addition to the high prevalence of single infection of HPV-16 in SCCA (50.9%), co-infection with HPV-16 was common among those with multiple HPV infections (9 out of 11).…”

Section: Discussionsupporting

confidence: 93%

“…Human papillomavirus (HPV) infection is a main cause for the development of cervical cancer (Liaw et al, 2001;Huang et al, 2004;Bhatla et al, 2008;Serrano et al, 2012). At present, more than 100 HPV genotypes have been identified (Huang et al, 2004;Kawana et al, 2012); among which 40 different types infecting the genital tract (Suthipintawong et al, 2011) are classified as high-risk type and associated with cervical carcinoma vs. the low-risk type associated with genital warts (Villa, 2006;Kawana et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…At present, more than 100 HPV genotypes have been identified (Huang et al, 2004;Kawana et al, 2012); among which 40 different types infecting the genital tract (Suthipintawong et al, 2011) are classified as high-risk type and associated with cervical carcinoma vs. the low-risk type associated with genital warts (Villa, 2006;Kawana et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Human Papillomavirus Genotypes and Cervical Cancer in Northeast Thailand

Natphopsuk¹,

Settheetham-Ishida²,

Pientong³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Human papillomavirus (HPV) is a major cause of cervical cancer. More than 100 HPV genotypes have been identified; however the distribution varies geographically and according to ethnicity. The purpose of this study was to investigate the prevalence and distribution of HPV subtypes among Northeast Thai women. Subjects included 198 cases of SCCA and 198 age-matched, healthy controls. HPV-DNA was amplified by PCR using the consensus primers GP5+/6+ system followed by reverse line blot hybridization genotyping. The prevalence of high-risk HPV infection was 21 (

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human Papillomavirus Genotypes and Cervical Cancer in Northeast Thailand

Natphopsuk¹,

Settheetham-Ishida²,

Pientong³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…In addition to other studies demonstrating that adenocarcinomas are more often HPV18 positive than HPV16 positive (Pilch et al, 2001;Schwartz et al, 2001;Altekruse et al, 2003;Burk et al, 2003;Clifford et al, 2003b;Huang et al, 2004), we have now shown that when comparing invasive adenocarcinoma cases to cytologically normal controls, the OR MH is only 1.3 for HPV16 and 15.0 for HPV18. Conversely, when comparing invasive SCC cases to normal controls, OR MH is 7.0 for HPV16 and only 1.3 for HPV18.…”

Section: Preferential Risk Of Hrhpv Types S Bulk Et Alsupporting

confidence: 72%

Preferential risk of HPV16 for squamous cell carcinoma and of HPV18 for adenocarcinoma of the cervix compared to women with normal cytology in The Netherlands

et al. 2005

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We present the type-distribution of high-risk human papillomavirus (HPV) types in women with normal cytology (n ¼ 1467), adenocarcinoma in situ (ACIS) (n ¼ 61), adenocarcinoma (n ¼ 70), and squamous cell carcinoma (SCC) (n ¼ 83). Cervical adenocarcinoma and ACIS were significantly more frequently associated with HPV18 (OR MH 15.0; 95% CI 8.6 -26.1 and 21.8; 95% CI 11.9 -39.8, respectively) than normal cytology. Human papillomavirus16 was only associated with adenocarcinoma and ACIS after exclusion of HPV18-positive cases (OR MH 6.6; 95% CI 2.8 -16.0 and 9.4; 95% CI 2.8 -31.2, respectively). For SCC, HPV16 prevalence was elevated (OR MH 7.0; 95% CI 3.9 -12.4) compared to cases with normal cytology, and HPV18 prevalence was only increased after exclusion of HPV16-positive cases (OR MH 4.3; 95% CI 1.6 -11.6). These results suggest that HPV18 is mainly a risk factor for the development of adenocarcinoma whereas HPV16 is associated with both SCC and adenocarcinoma. (Walboomers et al, 1999). Testing for hrHPV types combined with cervical cytology becomes increasingly attractive as data accumulate that a combined test increases the efficacy of cervical screening programmes and triage policies for women with both equivocal and normal cervical smears (Cuzick et al, 2003;Khan et al, 2005). Possibly, even more efficient screening strategies can be developed by selecting hrHPV types conferring a preferential risk for the development of cervical cancer, and treat these infections more aggressively. In order to assess the preferential risk for cervical cancer and its precursors, typespecific prevalence of hrHPV types in cancer cases should be compared to type-specific prevalence in women without cancer. In a meta-analysis of cervical squamous cell carcinomas (SCCs) compared to high-grade squamous intraepithelial lesions, HPV16, HPV18 and HPV45 appeared to display an elevated prevalence in cervical cancer (Clifford et al, 2003a). A second meta-analysis revealed that HPV16 and HPV18 are more prevalent in SCC than in low-grade SIL (Clifford et al, 2005). However, a comparison with hrHPV prevalence in women with normal cytology was not made, hampering the translation of these findings to implementation of type-specific hrHPV testing in population-based screening.Recently in a cross-sectional study, we have demonstrated that among the hrHPV types, HPV16 and HPV33 were significantly more common in women with cervical intraepithelial neoplasia grade 2 or more (XCIN2) than in women with normal cytology. However in that study, cases of XCIN2 were retrieved from population-based screening, and consequently, the prevalence of invasive carcinomas as well as adenocarcinoma in situ (ACIS) was low.In order to obtain a more comprehensive view on the change in distribution from hrHPV infections without cytological abnormalities to hrHPV prevalence in cervical cancer, we compared crosssectional screening data of women with normal cytology to retrospectively collected cases of SCC, adenocarcinoma (AdCx) and ACIS. MATERIALS AND METHODS Women wi...

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“…18 First, HPV DNA was amplified by PCR assay using modified MY11/GP6 þ biotinylated consensus primer sets to amplify a fragment of 192 bp in the L1 open reading frame of HPV and GAPDHF/GAPDHR biotinylated primer sets were used to amplify a 136-bp segment for specimen validation as described previously. 4,19 Briefly, PCR for HPV DNA was performed in a final reaction volume of 26 ml with 20-ng aliquot of genomic DNA in a PCR master mixture containing 15 mM Tris-HCl (pH 8.0), 2.0 mM MgCl 2 , 50 mM KCl, 0.25 mM each deoxynucleoside triphosphate, 0.6 mM primer, and 0.5 units of DNA polymerase (HPt High Performance HotStart Taq DNA Polymerase; DNA Technologies Ltd., UK). PCR assay was performed as follows: 10 min at 951C, followed by 40 cycles of 30 s at 951C, 30 s at 451C, and 30 s at 721C and a final extension of 5 min at 721C.…”

Section: Hpv Genotyping By Easychip Hpv Blotmentioning

confidence: 99%

Human papillomavirus genotyping for the eight oncogenic types can improve specificity of HPV testing in women with mildly abnormal Pap results

et al. 2008

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To evaluate whether human papillomavirus (HPV) genotyping for the selected oncogenic HPV types can improve the efficacy of HPV DNA testing in predicting cervical intraepithelial neoplasia (CIN 2/3) in women with mildly abnormal Pap results, we compared HPV DNA testing and HPV genotyping for eight oncogenic types (16, 18, 31, 33, 35, 45, 52 and 58) in Pap specimens with abnormal results (HSIL, 20 cases; LSIL, 42 cases; ASC-US, 94 cases) and follow-up biopsies. Using consensus primer-mediated PCR assays, HPV DNA was detected in 90% (18/20) of HSIL, 95% (40/42) of LSIL and 64% (60/94) of ASC-US cases. HPV DNA positivity was significantly associated with CIN 2/3/carcinoma (Po0.001) in women with ASC-US, but not in women with LSIL (P ¼ 0.52). Of HPV DNA-positive specimens, the eight oncogenic HPV types were detected in 83% of HSIL cases (15/18), 53% of LSIL cases (21/40), and 47% of ASC-US cases (28/60). The eight oncogenic HPV types were significantly associated with CIN 2/3/carcinoma (OR, 10.6; 95% CI, 3.98-28.10; Po0.001), whereas no significant association was observed between the non-eight oncogenic HPV types and CIN 2/3/carcinoma (OR, 2.20; 95%CI, 0.80-6.03; P ¼ 0.125). In women with ASC-US, HPV genotyping for the eight oncogenic types showed higher specificity (81 vs 46%) and positive predictive value (PPV, 44 vs 26%) in predicting CIN 2/3/carcinoma compared to HPV DNA testing. Similarly, in women with LSIL, higher specificity (69 vs 8%) and PPV (62 vs 39%) for predicting CIN 2/3/carcinoma were also observed using HPV genotyping test for the eight oncogenic types compared to HPV DNA testing. Our findings suggested that HPV genotyping for the eight oncogenic types might be useful to improve the efficacy of HPV DNA testing for predicting CIN 2/3/carcinoma in women with mildly abnormal Pap results, which may lead to personalized clinical management with improved patient compliance for follow-up. Modern Pathology (2008Pathology ( ) 21, 1037Pathology ( -1043 doi:10.1038/modpathol.2008 published online 23 May 2008 Keywords: human papillomavirus; HPV; genotyping; HSIL; LSIL; ASCUS Oncogenic HPV infection has been recognized as a primary risk factor responsible for more than 99% of cervical carcinoma and high-grade cervical precancerous lesions, that is, CIN 2/3.1 Persistent oncogenic HPV infection has been considered as the most important factor to determine cervical precancerous lesion progression. 2,3 In the United States, HPV DNA testing in conjunction with Pap cytology test has become standard practice for patient management in gynecology clinics and cervical cancer prevention programs. 4,5 Reflex oncogenic HPV DNA testing using pooled-probes has proved cost effective in the triage of women with atypical squamous cells of under-determined significance (ASC-US).6 Triage of women with low-grade squamous intraepithelial lesions (LSIL) using HPV DNA testing was recently recommended, but only for postmenopausal women. 4 The reason that HPV DNA testing was not recommended initially in the triage of women with LSIL...

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Multiple HPV genotypes in cervical carcinomas: improved DNA detection and typing in archival tissues

Cited by 55 publications

References 31 publications

Human Papillomavirus Genotypes and Cervical Cancer in Northeast Thailand

Human Papillomavirus Genotypes and Cervical Cancer in Northeast Thailand

Preferential risk of HPV16 for squamous cell carcinoma and of HPV18 for adenocarcinoma of the cervix compared to women with normal cytology in The Netherlands

Human papillomavirus genotyping for the eight oncogenic types can improve specificity of HPV testing in women with mildly abnormal Pap results

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