Multiple immunoregulatory disturbances in the progeny of rats with experimental gestational diabetes

Abstract

The objective of the present work was to study the morphofunctional state of insulin-expressing, antigen-presenting, Treg cells, epithelioreticulocytes, thymic cells expressing c-Fos and Bcl-2+ in the progeny of rats with experimental gestational diabetes (EGD). The methods employed included immunohistochemical staining with direct and indirect immunofluorescence using monoclonal antibodies against insulin, MHC-II antigen, CD25 antigen, cytokeratins, rat Bcl-2 and c-Fos proteins. The off-springs of rats with experimental gestational diabetes were shown to have multiple disturbances of immunoregulation in the thymus, such as abnormalities of Treg cell differentiation, impaired expression of early response proteins (c-Fos), reduced number of epithelioreticulocytes, antigen-presenting and insulin-immunopositive cells, hyperexpression of anti-apoptotic Bcl-2 protein. These changes interfere with the normal apoptotic process, selection of thymocytes, and development of central tolerance to pancreatic antigens.

Influence of experimental metabolic syndrome on bioimpedance spectroscopy indicators of rat body

“…Animals were anesthetized (thiopental sodium 45 mg/kg) for the electrodes insertion via hypodermic needles according to the standard scheme. After the matching color-coded leads were attached, 3-5 consecutive impedance measurements were taken with the average value calculation [4].…”

“…The extracellular to intracellular fluid volume ratio is 2:1 in male rats, whereas it is 1:1 in female rats. 4. Metabolic syndrome development on study day 7 is characterized by decrease in body fat mass in female rats and changes in the extracellular to intracellular fluid volume ratio following the "male" pattern -2:1, which should be considered as a sign of intracellular hyperhydration.…”