Multiple Label-Free Detection of Antigen−Antibody Reaction Using Localized Surface Plasmon Resonance-Based Core−Shell Structured Nanoparticle Layer Nanochip

Abstract

In this research, a localized surface plasmon resonance (LSPR)-based bioanalysis method for developing multiarray optical nanochip suitable for screening bimolecular interactions is described. LSPR-based label-free monitoring enables to solve the problems of conventional methods that require large sample volumes and time-consuming labeling procedures. We developed a multiarray LSPR-based nanochip for the label-free detection of proteins. The multiarray format was constructed by a core-shell-structured nanoparticle layer, which provided 300 nanospots on the sensing surface. Antibodies were immobilized onto the nanospots using their interaction with Protein A. The concentrations of antigens were determined from the peak absorption intensity of the LSPR spectra. We demonstrated the capability of the array measurement using immunoglobulins (IgA, IgD, IgG, IgM), C-reactive protein, and fibrinogen. The detection limit of our label-free method was 100 pg/mL. Our nanochip is readily transferable to monitor the interactions of other biomolecules, such as whole cells or receptors, with a massively parallel detection capability in a highly miniaturized package. We anticipate that the direct label-free optical immunoassay of proteins reported here will revolutionize clinical diagnosis and accelerate the development of hand-held and user-friendly point-of-care devices.

Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices

“…So far, most of the plasmonic biosensors have relied on antibodies as target recognition elements due to the selectivity and sensitivity of antibodies 17 . Although antibodies offer excellent molecular recognition capabilities, they do suffer from: (i) limited pH and temperature stability; loss of conformation and recognition functionality in non-aqueous media; (iii) high cost associated with generating antibodies; and (iv) poor compatibility with micro and nanofabrication processes for efficient integration with various transduction platforms.…”

A multiplexed immunoaggregation biomarker assay using a two-stage micro resistive pulse sensor

“…1932-1058/2016/10(2)/024109/11/$30.00 V C 2016 AIP Publishing LLC 10, 024109-1 colorimetric 32,33 ), electrochemical, 23,[34][35][36] SPR, [37][38][39][40] surface-enhanced Raman scattering (SERS), 41,42 and capillary electrophoretic immunoassays (CEIA). 43,44 However, these methods require complex setups, expensive detectors, and/or complicated encoding and decoding process, 45 making them impediment for point-of-care biomarker detection for applications including disease diagnosis and therapy monitoring.…”

A localized surface plasmon resonance based immunosensor for the detection of casein in milk

“…In recent years, immunosensors based on surface plasmon resonance (SPR) were developed for the measurement of antigens, which could bind to antibodies immobilized on the biosensor surface [9][10][11]. Immunosensors based on localized surface plasmon resonance (LSPR) have become strong candidates for the development of miniaturized high-throughput devices [12].…”

“…For the improvement of these issues, we developed a gold-capped nanoparticle substrate and achieved the label-free detection of the fibrinogen and anti-fibrinogen antibody reactions, peptide nucleic acid (PNA)-DNA and DNA-DNA hybridization [24,25]. Multi-detection of clinically important proteins was also performed by spotting different antibodies on the surface of our device [12]. In its construction, the silica nanoparticle was used as the ''core'', and thin gold films were used as the ''shell'' coated at the bottom and the top of the ''core'' (Fig.…”

“…Using protein A in the antibody immobilization procedure, our method is promising for orientating the anti-casein antibody on the sensor surface, thus making this approach become simple, effectively and sensitively [12,24]. The anticasein antibody was immobilized on the gold-capped nanoparticle substrate surface and the optimization as well as characterization of the casein immunosensor was then performed in the following sections.…”