Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

Handa, Shweta; Sharma, Nivedita; Pathania, Shruti

doi:10.3390/fermentation2020010

Cited by 34 publications

(20 citation statements)

References 30 publications

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“…Wheat bran has been reported to be mainly used by many researchers as the best inducer for xylanase and pectinase production . Apart from wheat bran, various other agroresidues such as rice bran , barley bran , corncob , wheat straw , rice straw , sorghum straw , maize straw , paddy husk , cassava bagasse , apple pomace , banana waste , sugarcane baggases , sweet sorghum baggase , and oil‐palm‐empty‐bunches have been used for xylanase production and orange/citrus peel , rice straw, orange pulp , banana peel , sugarcane bagasse , sugar beet pulp , and pineapple residue for pectinase production by different microorganisms.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Kaur

Varghese

Mahajan

2019

Biotech and App Biochem

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Simultaneous production of alkaline xylanase and all seven types of pectinases by a bacterial isolate, under solid-state fermentation was checked in this study. Under optimized conditions, high concurrent production of xylanase (22,800 ± 578 IU/g substrate) and pectinase (4,832 ± 189 IU/g substrate) was achieved. The different types of pectinases produced were exo-polymethylgalacturonase (782 IU/g), endo-polymethylgalacturonase (6.42 U/g), exo-polygalacturonase (2,250 IU/g), endo-polygalacturonase (11.57 U/g), polymethylgalacturonate lyase (53.99 IU/g), polygalacturonate lyase (59.78 IU/g), and pectin esterase (5.78 IU/g). Wheat bran resulted in the highest titer of both enzymes. The maximum xylanase-pectinase yield was detected after 7 days of incubation with 2 mM MgSO 4 and 1.5 g/L K 2 HPO 4 at wheat bran to moisture ratio 1:1.5 (w/v), media to flask volume ratio 1:25, pH 7.0, temperature 37 • C, and inoculum size 15%. Xylanase was most stable at pH 8.0, retained more than 75% activity up to 24 H, whereas pectinase was most stable at pH 9.0, having full activity even after 24 H. At 45 • C, the xylanase showed 82% residual activity after 6 H of incubation. The pectinase was 97% and 61% stable up to 3 H at 50 and 55 • C, respectively. This is the first report showing the production of xylanase-pectinases by bacterium along with high titer of seven types of pectinases, suitable for industries.

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Section: Resultsmentioning

confidence: 99%

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Kaur

Varghese

Mahajan

2019

Biotech and App Biochem

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“…The absorbance of the filtered juices was recorded (650 nm ) to elucidate the effect of pectinase on fruit juices. Enzyme was not applied in the control and commercial pectinase (Sigma, U.S.A) was used as standard …”

Section: Methodsmentioning

confidence: 99%

Simultaneous production of commercial enzymes using agro industrial residues by statistical approach

Viayaraghavan¹,

Kumar²,

Arasu

et al. 2019

J Sci Food Agric

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BACKGROUND Simultaneous production of commercial enzymes using agro‐industrial residues by statistical approach is an important perspective in an industrial point of view. Despite the advantages of statistical methods optimization, the report on simultaneous production of pectinase and amylases are limited. The accumulation of agro‐industrial residues causes serious environmental problems; however, citrus peel can be the important substrate for various enzymes production, including pectinase. These enzymes involving saccharification process and act as clarifying agent. RESULTS In this study, orange peel and banana peel mixture were used as the suitable substrate for pectinase and amylase production using Bacillus pumilus in solid‐state culture. The process parameters were optimized for simultaneous production of enzymes by a traditional‐one‐variable‐at‐a‐time approach, a two level full factorial design, central composite design and response surface methodology. Among the selected variables, moisture content of the medium, pH and mineral supplement significantly influenced pectinase and amylase production. Pectinase production increased over 3‐fold, whereas, 2‐fold increase on amylase production was achieved after optimization by statistical approach. The purified pectinase exhibited maximal activity at pH 8.0, temperature of 60 °C and the molecular weight was 60 kDa. The purified amylase was highly active at pH 8.0, at 50 °C and the molecular weight was 37 kDa. The enzyme showed activity on fruit pulp in increasing clarity in orange and carrot juice and the saccharification of starch. CONCLUSION Orange peel and banana peel mixture was effective as a solid medium for the simultaneous production of pectinase and amylase by Bacillus pumilus. Also, our statistical approach to optimize the medium components to yield more pectinase and amylase was fruitful and these enzymes showed appreciable results suitable for various applications. © 2018 Society of Chemical Industry

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“…Likewise, the achieved results are in agreement with those stated by Tari et al(2007) where they indicated that the pvalue reveals the correlation amongst the variables, and the response variable, pvalue lower than 0.05, shows that the practical model is significant. The model Fvalue and Probability value (P model > F) of 170.45 and <0.0001 respectively, for pectinase activity, show that the applied model is highly significant(Handa et al (2016). Likewise,(Asia et al 2019) reported that the significance of the model (p<0.05) was determined by Analysis of variance (ANOVA) that indicated an F-value of 8.22.…”

mentioning

confidence: 94%

Optimizing the Production of Pectinase of Orange Peel Waste by Penicillium Chrysogenum Mf318506 Using Response Surface Methodology in Submerged Fermentation

Ahmed¹,

Rahman²

2021

J microb biotech food sci

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Pectinases find wide application in different industrial applications, e.g., textile, food, tea, paper and pulp, vegetable oil extractions, saccharification of agricultural remains, and in fermented drinks. Three fungal strains were screened for pectinase production. Results indicated that all strains are capable of producing a pectinase enzyme. Penicillium chrysogenum MF318506 showed the highest pectinase activity (0.214 U/ml) on the recommended medium at 30 ◦C at 150 rpm, on 6 days. Different agricultural wastes (orange peel, banana peel, potato peel, pomegranate peel, wheat bran, and rice bran) were screened as a carbon source. The maximum pectinase activity of 0.48U/ml was obtained from Orange peel and was selected for further studies. Screening of important medium components for pectinase production by the Plackett-Burman design (PBD) showed that the maximum pectinase activity of 1.057 U/ml was achieved with 3 % orange peel, 2.0 g/l (NH4)2SO4, 6.0 g/l peptone, 6.0 g/l KH2PO4, 6.0 g/l K2HPO4, 0.1 g/l MgSO4.7H2O, 0.5 g/l KCl and 0.1 g/l FeSO4. Finally, the Box-Behken design (BBD) which uses the response surface methodology (RSM) was applied to further optimization of the selected factors using the PBD. The maximum pectinase activity of 1.292U/ml was observed at 2.5% orange peel and 4.5 g/l peptone. The Statistical optimization, enhanced the pectinase production by 6.04 folds using Orange peel waste as a substrate. The pectinase produced by Penicillium chrysogenum MF318506 has potential applications in different industrial fields.

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Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

Cited by 34 publications

References 30 publications

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Simultaneous production of commercial enzymes using agro industrial residues by statistical approach

Optimizing the Production of Pectinase of Orange Peel Waste by Penicillium Chrysogenum Mf318506 Using Response Surface Methodology in Submerged Fermentation

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