Multiple Pathways for Signaling Glutamate Taste in Rodents

Chaudhari, Nirupa; Maruyama, Yutaka; Roper, Stephen D.; Trubey, Kristina R.

doi:10.1093/chemse/bjh097

Cited by 7 publications

(4 citation statements)

References 19 publications

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“…This could be explained by the fact that these amino acids bind to the following: 1) the T1R3 protein at a site that is not affected by the Tas1r3 polymorphisms, 2) a partner protein (T1R1 or T1R2) of the heterodimeric T1R receptor, or 3) an additional, non-T1R, taste receptor. Several studies suggested that the taste of L-glutamate is transduced not only by the T1Rdependent mechanism but also by alternative transduction mechanisms (69,(78)(79)(80)(81)(82)(83). Similarly, T1R-independent mechanisms may be involved in taste transduction of the other amino acids, for which taste responses are not affected by Tas1r3 allelic variation.…”

Section: -Bottle Choice Testsmentioning

confidence: 99%

Genetics of Amino Acid Taste and Appetite

Bachmanov

Bosak

Glendinning

et al. 2016

Advances in Nutrition

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The consumption of amino acids by animals is controlled by both oral and postoral mechanisms. We used a genetic approach to investigate these mechanisms. Our studies have shown that inbred mouse strains differ in voluntary amino acid consumption, and these differences depend on sensory and nutritive properties of amino acids. Like humans, mice perceive some amino acids as having a sweet (sucrose-like) taste and others as having an umami (glutamate-like) taste. Mouse strain differences in the consumption of some sweet-tasting amino acids (d-phenylalanine, d-tryptophan, and l-proline) are associated with polymorphisms of a taste receptor, type 1, member 3 gene (Tas1r3), and involve differential peripheral taste responsiveness. Strain differences in the consumption of some other sweet-tasting amino acids (glycine, l-alanine, l-glutamine, and l-threonine) do not depend on Tas1r3 polymorphisms and so must be due to allelic variation in other, as yet unknown, genes involved in sweet taste. Strain differences in the consumption of l-glutamate may depend on postingestive rather than taste mechanisms. Thus, genes and physiologic mechanisms responsible for strain differences in the consumption of each amino acid depend on the nature of its taste and postingestive properties. Overall, mouse strain differences in amino acid taste and appetite have a complex genetic architecture. In addition to the Tas1r3 gene, these differences depend on other genes likely involved in determining the taste and postingestive effects of amino acids. The identification of these genes may lead to the discovery of novel mechanisms that regulate amino acid taste and appetite.

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Section: -Bottle Choice Testsmentioning

confidence: 99%

Genetics of Amino Acid Taste and Appetite

Bachmanov

Bosak

Glendinning

et al. 2016

Advances in Nutrition

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Section: Plos Geneticsmentioning

confidence: 88%

A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli

et al. 2020

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Taste receptor cells use multiple signaling pathways to detect chemicals in potential food items. These cells are functionally grouped into different types: Type I cells act as support cells and have glial-like properties; Type II cells detect bitter, sweet, and umami taste stimuli; and Type III cells detect sour and salty stimuli. We have identified a new population of taste cells that are broadly tuned to multiple taste stimuli including bitter, sweet, sour, and umami. The goal of this study was to characterize these broadly responsive (BR) taste cells. We used an IP 3 R3-KO mouse (does not release calcium (Ca 2+) from internal stores in Type II cells when stimulated with bitter, sweet, or umami stimuli) to characterize the BR cells without any potentially confounding input from Type II cells. Using live cell Ca 2+ imaging in isolated taste cells from the IP 3 R3-KO mouse, we found that BR cells are a subset of Type III cells that respond to sour stimuli but also use a PLCβ signaling pathway to respond to bitter, sweet, and umami stimuli. Unlike Type II cells, individual BR cells are broadly tuned and respond to multiple stimuli across different taste modalities. Live cell imaging in a PLCβ3-KO mouse confirmed that BR cells use this signaling pathway to respond to bitter, sweet, and umami stimuli. Short term behavioral assays revealed that BR cells make significant contributions to taste driven behaviors and found that loss of either PLCβ3 in BR cells or IP 3 R3 in Type II cells caused similar behavioral deficits to bitter, sweet, and umami stimuli. Analysis of c-Fos activity in the nucleus of the solitary tract (NTS) also demonstrated that functional Type II and BR cells are required for normal stimulus induced expression.

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“…In addition to these studies, multiple laboratories have independently concluded that the identified taste receptors are not solely responsible for transducing all bitter, sweet and umami stimuli. For instance, the detection of some carbohydrates is not impaired by the loss of T1R2 or T1R3, the identified sweet receptors (Kusuhara, Yoshida et al, 2013, Ohkuri, Yasumatsu et al, 2009, Treesukosol, Blonde et al, 2009, Treesukosol, Smith et al, 2011, Treesukosol & Spector, 2012, Zukerman, Glendinning et al, 2009), and umami stimuli appear to be transduced by receptors in addition to the T1R1+T1R3 heterodimer (Chaudhari, Landin et al, 2000, Chaudhari, Maruyama et al, 2005, Chaudhari, Pereira et al, 2009, Delay, Hernandez et al, 2006, Eddy, Eschle et al, 2017, Maruyama, Pereira et al, 2006, Pal Choudhuri, Delay et al, 2015, Pal Choudhuri, Delay et al, 2016, Shigemura, Shirosaki et al, 2009). The glucose transporter which has been implicated in detecting sweet stimuli (Sukumaran, Yee et al, 2016), was also expressed in some Type III cells in the RNA-seq profiling study.…”

Section: Discussionmentioning

confidence: 98%

A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli

Banik

Benfey

[]

et al. 2019

Preprint

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1Taste receptor cells use multiple signaling pathways to detect chemicals in 2 potential food items. These cells are functionally grouped into different types: Type I 3 cells act as support cells and have glial-like properties; Type II cells detect bitter, sweet, 4 and umami taste stimuli; and Type III cells detect sour and salty stimuli. We have 5 identified a new population of taste cells that are broadly tuned to multiple taste stimuli 6 including bitter, sweet, sour and umami. The goal of this study was to characterize 7 these broadly tuned (BR) taste cells. We used an IP3R3-KO mouse (does not release 8 calcium (Ca 2+ ) from Type II cells when stimulated with bitter, sweet or umami stimuli) to 9 characterize the BR cells without any potentially confounding input from Type II cells.

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Multiple Pathways for Signaling Glutamate Taste in Rodents

Cited by 7 publications

References 19 publications

Genetics of Amino Acid Taste and Appetite

Genetics of Amino Acid Taste and Appetite

A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli

A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli

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