Multiple roles of microRNA‑146a in immune responses and hepatocellular carcinoma (Review)

Li, Xuemei; Li, Tao; Wang, Lianzi; Wu, Xian; Liu, Jiaqing; Xu, Yuanhong; Wei, Wei

doi:10.3892/ol.2019.10862

Cited by 35 publications

(31 citation statements)

References 76 publications

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“…4,16 3 | miR-146a IN HBV/HCV Hepatitis C virus (HCV) and hepatitis B virus (HBV) infections are the most significant cause of liver fibrosis, cirrhosis, and hepatocellular carcinoma, and have become one of the most harmful viral infectious diseases worldwide. 17 HCV/HBV-associated pathology is principally due to some immunological reasons rather than the HBV/HCV itself. 18,19 Both the viral infection outcome and antiviral treatment efficacy are primarily dependent on viral/host immune factors.…”

Section: Mir-146amentioning

confidence: 99%

The role of miR‐146a in viral infection

et al. 2019

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Cellular microRNAs (miRNAs) were identified as a key player in the posttranscriptional regulation of cellular‐genes regulatory pathways. They also emerged as a significant regulator of the immune response. In particular, miR‐146a acts as an importance modulator of function and differentiation cells of the innate and adaptive immunity. It has been associated with disorder including cancer and viral infections. Given its significance in the regulation of key cellular processes, it is not surprising which virus infection have found ways to dysregulation of miRNAs. miR‐146a has been identified in exosomes (exosomal miR‐146a). After the exosomes release from donor cells, they are taken up by the recipient cell and probably the exosomal miR‐146a is able to modulate the antiviral response in the recipient cell and result in making them more susceptible to virus infection. In this review, we discuss recent reports regarding miR‐146a expression levels, target genes, function, and contributing role in the pathogenesis of the viral infection and provide a clue to develop the new therapeutic and preventive strategies for viral disease in the future.

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Section: Mir-146amentioning

confidence: 99%

The role of miR‐146a in viral infection

et al. 2019

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“…At present, miRNAs have been increasingly found to have important roles in the development of cancer. it has been shown that miR-146a not only has a tumor-suppressive effect, but also plays a critical role in the growth of HCC cells and that genetic variation of miR-146a may be a risk factor for developing Hcc (21). The expression of mir143HG was significantly downregulated in HCC cells and tissues, and was associated with the staging and prognosis of patients with Hcc (22).…”

Section: Discussionmentioning

confidence: 99%

miR‑378 in combination with ultrasonic irradiation and SonoVue microbubbles transfection inhibits hepatoma cell growth

Wang

et al. 2020

Mol Med Report

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ultrasonic microbubbles in combination with microrna (mirnas/mirs) exhibited promising effects on cancer treatments. The aim was to investigate the role of miR-378 in hepatoma cells and the efficiency of it in combination with ultrasonic irradiation and SonoVue ® microbubbles method for cell transfection. HuH-7, Hep3B and SK-Hep1 cells were transfected with an miR-378 mimic using only lipofectamine ® 3000 or combined with SonoVue microbubbles and ultrasonic irradiation at 0.5 W/cm 2 for 30 sec. mRNAs and protein levels of Cyclin D1, Bcl-2, Bax, Akt, p53 and Survivin were detected by reverse transcription-quantitative PCR and western blotting, respectively. Cell survival rate, proliferation, cell cycle and apoptosis were determined by Cell Counting Kit-8, cell double cytochemical staining and flow cytometry, respectively. It was found that using a combination of ultrasonic irradiation and the SonoVue microbubbles method increased the effectiveness of mir-378 transfection into hepatocellular carcinoma (Hcc) cells, and increased the inhibition of cell survival and proliferation. Moreover, mir-378 increased the rate of apoptosis and upregulated the expression of Bax and p53, and suppressed the cell cycle and downregulated the expression of Cyclin D1, Bcl-2, Akt, β-catenin and Survivin much more effectively in the HCC cell line by applying the combined method. Thus, miR-378 was shown to be a suppressive factor to reduce proliferation and increase apoptosis in HCC cells. Additionally, the combination of ultrasonic irradiation and SonoVue microbubbles method was more efficient in the transfection of miRNA.

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“…21,22 Also, miR-146a has been found function as an oncogene in hepatocellular carcinoma. 14,15 However, the potential mechanism of miR-164a in retinoblastoma still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…[7][8][9][10][11][12][13] Recent years, more and more studies have found that miR-146a also act as an oncogene in hepatocellular carcinoma. 14,15 Much attentions have been paid to miRNAs in retinoblastoma, however, the pathological correlation of miR-146a in retinoblastoma was poorly knew. Thus, our study mainly focused on the expression of miR-146a in retinoblastoma and aiming to reveal its potential mechanism.…”

Section: Introductionmentioning

confidence: 99%

MiR‐146a functions as a potential tumor suppressor in retinoblastoma by negatively regulate neuro‐oncological ventral antigen‐1

Liu

2020

The Kaohsiung J of Med Scie

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MicroRNAs (miRNAs) are dysregulated in many tumors and have been found to play crucial roles in cancer biology. Retinoblastoma is a rare tumor that develops rapidly from a malignant tumor of immature cells in the retina known as photoreceptor progenitors. Our study aimed to explore the role of miR-146a in the pathology of retinoblastoma. Potential target gene of miR-146a was predicted by Targetscan. Reverse transcription quantitative polymerase chain reaction (RT-PCR) showed that miR-146a was downregulated and ventral nerve tumor antigen 1 (Neuro-oncological ventral antigen 1, NOVA1) was upregulated in retinoblastoma. Luciferase assay confirmed that miR-146a directly target NOVA1. MiR-146a knockdown and overexpression experiments were performed and found that miR-146a could regulate the expression of NOVA1. The miR-146a knockdown and overexpression experiments were conducted to investigate the biological function of miR-146a. MiR-146a was found inhibited the viability, proliferation and invasion of retinoblastoma cell by MTT, EdU, and transwell assays. Flow cytometry was performed for the apoptosis analysis and miR-146a increased the apoptosis of retinoblastoma cell was found. Above phenomenon can be rescued by overexpression of NOVA1. In conclusion, these results suggest that miR-146a acts as a tumor suppressor and can act as a potential therapeutic target for retinoblastoma in the future.

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Multiple roles of microRNA‑146a in immune responses and hepatocellular carcinoma (Review)

Cited by 35 publications

References 76 publications

The role of miR‐146a in viral infection

The role of miR‐146a in viral infection

miR‑378 in combination with ultrasonic irradiation and SonoVue microbubbles transfection inhibits hepatoma cell growth

MiR‐146a functions as a potential tumor suppressor in retinoblastoma by negatively regulate neuro‐oncological ventral antigen‐1

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