Multiple roles of oxidants in the pathogenesis of asbestos-induced diseases

The Role of Inflammation in Development and Therapy of Malignant Mesothelioma

“…Further studies in human mesothelial cells confirmed that asbestos exposure caused priming of NLRP3 (increased mRNA levels) as well as its activation (Shukla et al, 2003;2011). In support of our work, Yang et al (2010) showed the release of high mobility group box-1 protein (HMGB-1) from asbestosexposed mesothelial cells (Yang et al, 2010).…”

“…expression (Janssen-Heininger et al, 2000;Shukla et al, 2003). Our group has repeatedly demonstrated the role of asbestos in inflammation.…”

“…A survey of the current literature suggests that there is more than one mechanism involved in the pathogenesis of asbestosinduced mesothelioma. One proposed mechanism is the oxidative stress concept that highlights how iron within asbestos fibers catalyzes free radical generation and thereby induces oxidative stress and carcinogenesis (Shukla et al, 2003). Another proposed mechanism is the chronic inflammatory theory that is founded on the observation that inflammation is a biologic response to pathogenic materials and injured cells in which various types of cells, such as neutrophils, macrophages, fibroblasts and vascular endothelial cells, interact with each other.…”

Dispersion medium modulates oxidative stress response of human lung epithelial cells upon exposure to carbon nanomaterial samples

“…ROS generation through Fenton reactions or following phagocytosis has been directly related to fibre toxicity and has also been associated with carbon nanotube (CNT) exposure, leading to lipid peroxidation, oxidative stress and changes in cell morphology [Sayes et al, 2005;Shvedova et al, 2003]. Other consequences of oxidative injury include effects on nuclear factor activation, gene transcription and protein [Shukla et al, 2003;Vallyathan and Shi, 1997. This study investigates the oxidative stress response following in vitro exposure to three types of carbon nanomaterials, high pressure carbon monoxide produced SWCNT (HiPco SWCNT), arc discharge produced SWCNT and P90 carbon black, following dispersion in biological media. As-produced nanomaterials are employed, unpurified and minimally processed, in order to best mimic occupation exposure by inhalation of airborne dust particles derived from carbon nanoparticle production.…”

Gene expression and copy number profiling suggests the importance of allelic imbalance in 19p in asbestos-associated lung cancer

“…The mechanisms behind these actions may be multiple. The main mechanisms are thought to be generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS), alteration in the mitochondrial function, physical disturbance of cell cycle progression and activation of several signal transduction pathways (Upadhyay and Kamp, 2003;Shukla et al, 2003a). Asbestos-exposed workers have been reported to have increased levels of sister chromatid exchanges and DNA double-strand breaks in white blood cells (Fatma et al, 1991;Marczynski et al, 1994).…”