Multiple Shoot-Bud Stimulation and Rhizogenesis Induction of Embryogenic and Juvenile Explants of Walnut

Peñuela, Rosa Magdalena Uscátegui; Garavito, Cesar Hernán Rodríguez; Tamés, Ricardo Sánchez; Rodrı́guez, Ricardo

doi:10.17660/actahortic.1988.227.92

Cited by 11 publications

(5 citation statements)

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“…;Chalupa, (1981) andPenuela et al, (1987) who confirmed the importance of the auxin NAA in the rooting process.Table (7): Effect of medium salt strength and NAA concentrations on the average number of roots/shoot. c 3.00 b 3.13 b 3.25 b 4.25 a Overall mean = 2.73…”

mentioning

confidence: 84%

Effect of Different Concentrations of Kinetin and NAA on Micropropagation of Gardenia Jasminoides

M.S.Duhoky¹,

Rasheed²

2009

JZS

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The goal of this study was to use plant tissue culture technique in vegetative propagation of Gardenia (Gardenia jasminoides) by using single nodes and shoot tips excised from soft cuttings and treated with different concentrations of growth regulators.The results revealed that the use of different disinfistants was highly effective in reducing cultures contamination. The use of mercuric chloride (0.1%, HgCl2) for 10 minutes was very effective in preventing contamination and gave the highest survival percentage (99%). During the culture of shoot tips at initiation stage, a nutrient medium contained (2 mgl-1 kinetin+ 0.6 mgl-1 NAA) gave the highest values of average number of shoots and leaves and growth length (2.3 shoots/ explant, 2.4 leaves and 1.9 cm respectively).At vegetative multiplication stage, The culture of shoot tips on a nutrient medium containing (3 mgl-1 kinetin) gave the highest values of average number of shoots and leaves (2.9 shoots/ explant, 3.6 leaves respectively).As for growth length, the results revealed that the highest value was achieved by MS medium supplemented by 2 mgl-1 kinetin + 0.1 mgl-1 NAA (3.3 cm). Regarding rooting process, MS medium supplemented by 4 mgl-1 NAA gave the highest average number and length of roots (4.0 roots and 2.50 cm respectively).Also, the medium supplemented by 8 mgl-1 IAA gave the highest number and length of roots (3.40 roots and 3.50 cm respectively). Plantlets obtained were transferred to pots and acclimatized with 95 % success.

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confidence: 84%

Effect of Different Concentrations of Kinetin and NAA on Micropropagation of Gardenia Jasminoides

M.S.Duhoky¹,

Rasheed²

2009

JZS

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“…esto se puede lograr mediante: Pretratamientos en especies que generan exudados con efectos perjudiciales, la implementación de un pretratamiento aplicado al explante puede ser de mucha utilidad. Por ejemplo, Pérez et al (1985) y Peñuela et al (1988) mencionan la utilidad de aplicar un baño de agua continua durante 16 a 24 horas para la remoción de sustancias fenólicas producidas en semillas de Corylus sp. y Juglans sp., respectivamente, antes de su desinfección.…”

Section: Remoción De Sustancias Fenólicasunclassified

Problemas de oxidación y oscurecimiento de explantes cultivados in vitro

Azofeifa

2008

Agron. Mesoam.

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“…It is important to call attention to earlier work on zygotic embryos of walnut by Cossio and Minotta (1983), Rodriguez and Sanchez-Tames (1981), and Rodriguez (1982), as well related recent publications by Jay-Allemand and Cornu (1986), Penuela et al (1988), Rodriquez et al (1989, and the work on apomixis in walnut by Deryuzhkin et al (1985). These studies contribute to the general advance of our understanding of walnut embryogenic development.…”

Section: Discussionmentioning

confidence: 94%

Somatic Embryogenesis and Synthetic Seed I

1995

Biotechnology in Agriculture and Forestry

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Multiple Shoot-Bud Stimulation and Rhizogenesis Induction of Embryogenic and Juvenile Explants of Walnut

Cited by 11 publications

References 0 publications

Effect of Different Concentrations of Kinetin and NAA on Micropropagation of Gardenia Jasminoides

Effect of Different Concentrations of Kinetin and NAA on Micropropagation of Gardenia Jasminoides

Problemas de oxidación y oscurecimiento de explantes cultivados in vitro

Somatic Embryogenesis and Synthetic Seed I

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