Multiple tissue gene expression analyses in Japanese medaka (Oryzias latipes) exposed to hypoxia

Zhang, Ju; Wells, Melissa C.; Heater, Sheila; Walter, Ronald B.

doi:10.1016/j.cbpc.2006.06.012

Cited by 60 publications

(59 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is reflected by gene expression changes observed in other fish exposed to hypoxia (Gracey et al, 2001;Ton et al, 2003;David et al, 2005;Ju et al, 2007). In our study, no genes involved in TCA cycle and the electron transfer chain were found to be induced.…”

Section: Discussionsupporting

confidence: 60%

“…A range of physiological adaptations on transcriptional levels have received considerable attention in fish. cDNA microarray has been used to identify hypoxiainduced genes in tissues from several fish species (Gracey et al, 2001;Gracey and Cossins, 2003;Ton et al, 2003;David et al, 2005;Ju et al;2007). Suppression subtractive hybridization is a powerful approach to identify differentially expressed gene that involved in physiological and pathological processes without bias of the prerequisite knowledge of a specific gene.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization

Zhong

Wang

Zhang

et al. 2009

Comparative Biochemistry and Physiology Part B: Biochemistry an

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization

Zhong

Wang

Zhang

et al. 2009

Comparative Biochemistry and Physiology Part B: Biochemistry an

View full text Add to dashboard Cite

“…Groups with similar letters are significantly different from each other with p< 0.05 (two-way ANOVA with Bonferroni post hoc test). Significant differences of Le-SOD expression levels between the groups of small individuals were identified using an unpaired t test with Welch correction for unequal variances or the nonparametric Mann-Whitney test pathways involving NFκB as found in the disk abalone Haliotis discus discus (De Zoysa et al 2009;Ju et al 2007). Whereas some Antarctic marine invertebrates such as the sea star Odontaster validus or the gammarid Paraceradocus gibber lack a classical heat shock response (Clark et al 2008b;Clark and Peck 2009), experimental work with L. elliptica indicated that the HSP70 genes are induced in response to heat stress (Clark et al 2008a;Park et al 2007), hypoxia (Clark and Peck 2009;Clark et al 2013), and also changes in pH (Cummings et al 2011).…”

mentioning

confidence: 99%

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Husmann

Abele

Rosenstiel

et al. 2014

Cell Stress and Chaperones

View full text Add to dashboard Cite

Increasing temperatures and glacier melting at the Western Antarctic Peninsula (WAP) are causing rapid changes in shallow coastal and shelf systems. Climate change-related rising water temperatures, enhanced ice scouring, as well as coastal sediment runoff, in combination with changing feeding conditions and microbial community composition, will affect all elements of the nearshore benthic ecosystem, a major component of which is the Antarctic soft-shelled clam Laternula elliptica. A 454-based RNA sequencing was carried out on tissues and hemocytes of L. elliptica, resulting in 42,525 contigs, of which 48 % was assigned putative functions. Changes in the expression of putative stress response genes were then investigated in hemocytes and siphon tissue of young and old animals subjected to starvation and injury experiments in order to investigate their response to sedimentation (food dilution and starvation) and iceberg scouring (injury). Analysis of antioxidant defense (Le-SOD and Lecatalase), wound repair (Le-TIMP and Le-chitinase), and stress and immune response (Le-HSP70, Le-actin, and Letheromacin) genes revealed that most transcripts were more clearly affected by injury rather than starvation. The upregulation of these genes was particularly high in the hemocytes of young, fed individuals after acute injury. Only minor changes in expression were detected in young animals under the selected starvation conditions and in older individuals. The stress response of L. elliptica thus depends on the nature of the environmental cue and on age. This has consequences for future population predictions as the environmental changes at the WAP will differentially impact L. elliptica age classes and is bound to alter population structure.

show abstract

“…Customized cDNA microarrays PCR amplified cDNA fragments or synthetic oligonucleotide based on ESTs spotted on glass slides or nylon membranes (Ju et al, 2007).…”

Section: Array Based Platformsmentioning

confidence: 99%

Untitled

Hakim¹

2017

GenAqua

View full text Add to dashboard Cite

Although aquaculture management becomes challenging because of overuse, pollution and human activities reduced resources and genetic variations, current developments in molecular technology and genome programs have created an enormous evidence to be used for the genetic advancement of aquaculture. Over the past decade, using molecular markers showed polymorphism at the DNA level and played rising role in aquaculture. The utility of DNA markers has certified the hastiness in the exploration of fish germplasm. Various molecular markers present and their differences in principles, methodologies and applications still need careful consideration in selecting more useful marker for the successful management of aquaculture. In addition to the basic principles, necessities, pros and cons of various markers, this review also discusses their usage in different aspects of aquaculture; we provide an overview of the potential of various techniques of functional genomics like expressed sequence tags (EST), microarrays and RNA sequencing in aquaculture.

show abstract

Multiple tissue gene expression analyses in Japanese medaka (Oryzias latipes) exposed to hypoxia

Cited by 60 publications

References 66 publications

Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization

Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Untitled

Contact Info

Product

Resources

About