Multiple-type HPV infection predicting condyloma acuminatum recurrence after aminolevulinic acid photodynamic therapy

Hua, Hui; Zhou, Shu; Gu, Li; Shi, Zhinan; Gu, Li-Qun; Zhou, Bingrong

doi:10.1016/j.pdpdt.2021.102538

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…The CA lesions on both the skin and mucosa suggest the infection of multiple types of HPV 19 . A recent published study showed that the infection of multiple HPV types is an important clinical predictor of recurrence after photodynamic therapy 11 . Third, the preoperative mean CD4 cell count of the patients in our study was lower than that in Xu et al’s study.…”

Section: Discussioncontrasting

confidence: 67%

“…Xu et al 10 found that the CA recurrence rate in HIV‐positive patients treated with ALA‐PDT was approximately 29%. Although this rate is lower than that of other physical therapy methods, it is significantly higher than that of the normal population 11 . At present, there is no study on the clinical factors predicting the CA recurrence after ALA‐PDT treatment in HIV‐positive patients.…”

Section: Introductionmentioning

confidence: 85%

“…Although this rate is lower than that of other physical therapy methods, it is significantly higher than that of the normal population. 11 At present, there is no study on the clinical factors predicting the CA recurrence after ALA-PDT treatment in HIV-positive patients. CD4 + T lymphocytes (CD4 cells), the main target cells of HIV infection, play a central role in immune responses.…”

Section: Introductionmentioning

confidence: 99%

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Peripheral blood CD4⁺ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients

Zhou

Shi

et al. 2022

Photoderm Photoimm Photomed

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Background Few studies have reported postoperative relapse of condyloma acuminatum (CA) after 5‐aminolevulinic acid photodynamic therapy (ALA‐PDT) in human immunodeficiency virus (HIV) positive patients. Methods The clinical data of HIV‐positive CA patients treated with ALA‐PDT from October 2018 to December 2019 were analyzed retrospectively. Univariate and multivariate Cox regression was used to analyze the variables related to postoperative recurrence. Pearson correlation test was employed to analyze the correlation between CD4+ T cell count and postoperative recurrence rate. Kaplan–Meier method was used to compare the CA recurrence after ALA‐PDT in low CD4 group and high CD4 group. Results A total of 38 HIV‐positive patients with CA were included in the study. Among them, 26 patients experienced CA recurrence within 6 months, and the recurrence rate was 68.4%. CD4+ T cell count was 187.0 (79.0–596.0) cells/μl in relapsed patients and 406.0 (89.0–612.0) cells/μl in non‐relapsed patients, showing a statistically significant difference (p = .005). Pearson correlation coefficient analysis revealed a negative correlation between CD4+ T cell count and postoperative recurrence rate (p = .005, r = −.443). Univariate regression analysis showed that CD4+ T cell count was correlated with postoperative recurrence, hazard ratio (HR) was 0.99 [95% Confidence interval (CI) = 0.99–1.0, p = .012]. Multivariate Cox regression analysis showed that with the low CD4+ T cell count as the reference, the high CD4+ T cell count was negatively correlated with postoperative recurrence (HR = 0.09, 95% CI 0.01–0.87, p = .038). Conclusions Peripheral blood CD4+ T cell count can predict the CA recurrence rate after ALA‐PDT in HIV‐positive patients.

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Section: Discussioncontrasting

confidence: 67%

Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Peripheral blood CD4⁺ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients

Zhou

Shi

et al. 2022

Photoderm Photoimm Photomed

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“…5‐Aminolevulinic acid photodynamic therapy (ALA‐PDT) is currently used for skin diseases such as acne vulgaris, condyloma acuminatum and solar keratosis (Boen et al, 2017; Hua et al, 2021; Park et al, 2010; Zhou et al, 2019). ALA, once converted into PpIX in cells, can be further activated by light at certain wavelengths to induce the production of reactive oxygen species (ROS) (Kwiatkowski et al, 2018; Ozog et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Protective autophagy enhances antistress ability through AMPK/ULK1 signaling pathway in human immortalized keratinocytes

Shi,

Wang,

et al. 2024

Cell Biology International

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Keratinocytes, located in the outermost layer of human skin, are pivotal cells to resist environmental damage. Cellular autophagy plays a critical role in eliminating damaged organelles and maintaining skin cell homeostasis. Low‐dose 5‐Aminolevulinic acid photodynamic therapy (ALA‐PDT) has been demonstrated to enhance skin's antistress ability; however, the regulatory mechanisms of autophagy in keratinocytes remain unclear. In this study, we treated immortalized human keratinocytes (HaCaT cells) with low‐dose ALA‐PDT (0.5 mmol/L, 3 J/cm2). Through RNA‐sequencing analysis, we identified that low‐dose ALA‐PDT modulated autophagy‐related pathways in keratinocytes and pinpointed Unc‐51‐like kinase 1 (ULK1) as a key gene involved. Western blot results revealed that low‐dose ALA‐PDT treatment upregulated the expression of autophagy‐related proteins Beclin‐1 and LC3‐II/LC3‐I ratio. Notably, low‐dose ALA‐PDT regulated autophagy by inducing an appropriate level of reactive oxygen species (ROS), transiently reducing mitochondrial membrane potential, and decreasing adenosine triphosphate production; all these processes functioned on the AMP‐activated protein kinase (AMPK)/ULK1 pathway to activate autophagy. Finally, we simulated external environmental damage using ultraviolet B (UVB) at a dose of 60 mJ/cm2 and observed that low‐dose ALA‐PDT mitigated UVB‐induced cell apoptosis; however, this protective effect was reversed when using the autophagy inhibitor 3‐methyladenine. Overall, these findings highlight how low‐dose ALA‐PDT enhances antistress ability in HaCaT cells through controlling ROS generation and activating the AMPK/ULK1 pathway to arouse cellular autophagy.

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Preparation of transferrin-modified IR820-loaded liposomes and its effect on photodynamic therapy of breast cancer

Di,

Shuhe,

Baoding

et al. 2024

Discov Onc

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Objective To prepare and characterize transferrin (Tf)-modified liposomes (Lipo) encapsulating the photosensitizing agent neoindocyanine green (IR820), and to investigate their effects on breast cancer 4T1 cells as well as in a breast cancer mouse model. Methods Photosensitive liposomes, IR820@Lipo and Tf-IR820@Lipo, were synthesized using thin film dispersion, with encapsulation efficiency assessed via UV detection. The physicochemical properties were analyzed using transmission electron microscopy (TEM) and particle size analysis. Uptake by breast cancer 4T1 cells was evaluated through confocal laser scanning microscopy and flow cytometry, while cell proliferation inhibition was measured using the CCK8 assay. Differences in intracellular fluorescence intensity were utilized to assess drug aggregation in vivo through small animal imaging techniques. The anticancer efficacy and potential side effects of the formulations were examined through pharmacodynamic studies conducted in vivo . Results The mean particle sizes of IR820@Lipo and Tf-IR820@Lipo were found to be 84.30 ± 15.66 nm and 116.20 ± 14.68 nm respectively, with zeta potentials of −8.21 ± 2.06 mV for IR820@Lipo and −5.23 ± 1.19 mV for Tf-IR820@Lipo; TEM revealed that the liposomes exhibited a spheroid morphology with uniform distribution across samples; encapsulation efficiencies reached 86.38 ± 0.99% for IR820@Lipo and an impressive 93.81 ± 1.06% for Tf-IR820@Lipo; notably, Tf-IR820@Lipo significantly inhibited proliferation while promoting apoptosis of the 4T1 cells upon laser irradiation; reactive oxygen species (ROS) detection indicated enhanced fluorescence intensity within the treated cells under light exposure when utilizing both formulations; in vivo experiments demonstrated tumor accumulation of both IR820@Lipo and Tf-IR820@Lipo, indicating effective tumor targeting within a breast cancer mouse model; pharmacodynamic assessments revealed that Tf-IR820@Lipo exhibited superior inhibitory effects against breast cancer without causing liver or kidney dysfunctions in mice nor presenting significant toxic side effects overall. Conclusion Given its high targeting capability, potent efficacy, and low toxicity profile, Tf-IR820@Lipo holds promise as a novel therapeutic agent for breast cancer treatment when combined with photodynamic therapy (PDT), potentially offering new avenues for patient management.

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Multiple-type HPV infection predicting condyloma acuminatum recurrence after aminolevulinic acid photodynamic therapy

Cited by 6 publications

References 20 publications

Peripheral blood CD4⁺ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients

Peripheral blood CD4⁺ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients

Protective autophagy enhances antistress ability through AMPK/ULK1 signaling pathway in human immortalized keratinocytes

Preparation of transferrin-modified IR820-loaded liposomes and its effect on photodynamic therapy of breast cancer

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Multiple-type HPV infection predicting condyloma acuminatum recurrence after aminolevulinic acid photodynamic therapy

Cited by 6 publications

References 20 publications

Peripheral blood CD4+ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients

Peripheral blood CD4+ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients

Protective autophagy enhances antistress ability through AMPK/ULK1 signaling pathway in human immortalized keratinocytes

Preparation of transferrin-modified IR820-loaded liposomes and its effect on photodynamic therapy of breast cancer

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Peripheral blood CD4⁺ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients

Peripheral blood CD4⁺ T cell count predicts recurrence of condyloma acuminatum after photodynamic therapy in HIV‐positive patients