Multiples of Median-Transformed, Normalized Reference Ranges of Steroid Profiling Data Independent of Age, Sex, and Units

Zalas, Dominika; Reinehr, Thomas; Niedziela, Marek; Borzikowsky, Christoph; Flader, Maciej; Simic-Schleicher, Gunter; Akkurt, Halit Ilker; Heger, Sabine; Hornig, Nadine; Holterhus, Paul‐Martin; Kulle, Alexandra

doi:10.1159/000488028

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…With the aim of interpreting the acquired results, they were subsequently calculated on a scale of a standard ten, by applying the method frequently used in health psychology (other fields of science) which involves the transposing of data to standardized units. This enabled the comparison of results for the analysed people from a wide group of norms (standard results range between 1 and 10) (Zalas et al, 2018). The indicator of the level of intensity of health behaviour of the analysed group that is calculated in this way is placed at around 6 out of ten, thus it is possible to state that this result is not high, but rather average by comparison with the average values indicated in subject-related literature (Table1) (Juczyński, 2009).…”

Section: Sd -Standard Deviationmentioning

confidence: 99%

Untitled

2020

JPES

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Purpose: We know that the realization of the training aims is not only associated with a systematic effort, multiple skills, specific predispositions, yet also everyday health behaviour, namely various forms of behaviour of individuals that have an impact on the state of health. This influences its retention and strengthening. The aim of the research conducted was to increase knowledge relating to the ties between health behaviour of students in terms of physical education and their subjective evaluation of the progress in the sphere of their individual possibilities of adapting to psychophysical strain. Material: The research encompassed 65 students of physical education at M.A. level (average 21±1.56 years; range 19 -25 years). The measurement was conducted by applying the "Inventory of Health Behaviour Questionnaire", namely a standardized tool consisting of 4 subscales referring to various aspects of health behaviour that emerge in everyday life. Results: There is significant difference between age of both genders of participants. However, variance analysis did not reveal any significant differences between self-estimation and health behaviour aspects of participants. Only moderate correlation between self-estimation and health behaviour indices were statistically significant, which higher values in a group of females. Conclusions: A subjective evaluation of the progress made and the positive mental approach indicate the relation with the health behaviour of students of the physical education course. The results indicate that in the case of the analysed students in the sphere of the health behaviour shown, there is still great potential in terms of the possibilities to be availed of. The results acquired by us may lead to paying greater attention to the problematic issue of the awareness of the role of positive nutritional behaviour in the area of the course content conducted in the academic environment.

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Section: Sd -Standard Deviationmentioning

confidence: 99%

Untitled

2020

JPES

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“…Recent progress in liquid chromatography with tandem mass spectrometry (LC-MS/MS) technology allowed complete plasma steroidomic profiling for CAH patients (14,24,44,48) and revealed an increase in so called "minor compounds," including 21-deoxycorticosterone (21DB) and 21-deoxycortisol (21DF) originating from increased steroid precursors, respectively, progesterone and 17OHP (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Interaction between accumulated 21-deoxysteroids and mineralocorticoid signaling in 21-hydroxylase deficiency

Travers

Bouvattier

Fagart

et al. 2020

American Journal of Physiology-Endocrinology and Metabolism

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21-Hydroxylase deficiency (21OHD) is a rare genetic disorder in which salt-wasting syndrome occurs in 75% of cases, due to inability to synthesize cortisol and aldosterone. Recent mass spectrometry progress allowed identification of 21-deoxysteroids, i.e., 17-hydroxyprogesterone (17OHP), 21-deoxycortisol (21DF), and 21-deoxycorticosterone (21DB). We hypothesized that they may interfere with mineralocorticoid signaling and fludrocortisone therapy in patients with congenital adrenal hyperplasia (CAH) without effective glucocorticoid replacement and ACTH suppression. Our goal was to quantify circulating 21-deoxysteroids in a pediatric cohort with CAH related to 21OHD and to examine their impact on mineralocorticoid receptor (MR) activation. Twenty-nine patients with salt-wasting phenotype were classified in two groups according to their therapeutic control. During routine follow-up, 17OHP, 21DF, 21DB, and cortisol levels were quantified by liquid chromatography with tandem mass spectrometry before hydrocortisone intake and 1 and 2.5 h following treatment administration. Luciferase reporter gene assays were performed on transfected HEK293T cells while in silico modeling examined structural interactions between these steroids within ligand-binding domain of MR. Plasma 17OHP, 21DF, and 21DB accumulate in uncontrolled patients reaching micromolar concentrations even after hydrocortisone intake. 21DF and 21DB act as partial MR agonists with antagonist features similar to 17OHP, consistent with altered anchoring to Asn770 and unfavorable contact with Ala773 in ligand-binding pocket of MR. Our results demonstrate a complex interaction between all accumulating 21-deoxysteroids in uncontrolled 21OHD patients and mineralocorticoid signaling and suggest that appropriate steroid profiling should optimize management and follow-up of such patients, as keeping those steroids to low plasma levels should attest therapeutic efficacy and prevent interference with MR signaling.

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