Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish

Jarque, Sergio; Ibarra, Jone; Rubio-Brotons, Maria; García-Fernández, José M.; Terriente, Javier

doi:10.3390/ijms20071739

Cited by 24 publications

(8 citation statements)

References 80 publications

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“…In fact, in recent years, embryos from small fish, particularly zebrafish, rather than adults, have been used to develop and optimize high‐throughput screening methods to detect EDCs (Jarque et al, 2018; Jarque et al, 2019). There are not many studies on dosage and exposure at late stages, but studies should be performed on exposure lasting for a lifetime.…”

Section: Discussionmentioning

confidence: 99%

Does bisphenol A bioaccumulate on zebrafish? Determination of tissue bisphenol A level

Eser

Tural

Günal

et al. 2021

Biomedical Chromatography

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Bisphenol A (BPA) is a high‐production‐volume industrial chemical mainly used in the production of polycarbonates and epoxy resins utilized in the manufacture of containers, bottles, toys, and medical devices. It has systemic effects as an endocrine disruptor even at low doses. To analyze its quantity in biological materials, sensitive and reproducible methods have to be used. Different doses and duration (90 and 900 μg/L, 24 and 120 h, and 21 days) of BPA exposure to whole body zebrafish were analyzed after specific homogenization of tissue, and then a modified method HPLC was used. The mobile phase was acetonitrile and water using a gradient method of reversed‐phase C18 column, and excitation = 227 nm/emission = 313 nm. The calibration curve for BPA using HPLC‐fluorescence detection method was between a concentration range of 1 and 1000 ng/mL and linear, and r2 = 0.999. The mean and standard error of mean values were 4.29 ± 1.05, 2.50 ± 0.92, and 2.53 ± 0.68 for control; 10.43 ± 2.61, 11.46 ± 3.24, and 8.55 ± 3.11 for BPA‐90 μg/L; and 17.78 ± 4.39, 21.55 ± 4.37, and 25.32 ± 3.25 for BPA‐900 μg/L (24 h, 120 h, and 21 days, respectively). Although some statistical significance among dose/time was observed between two different dose‐treated groups, statistical significance was not found in dose/time results within the group. However, the positive result of BPA in the control group can be explained by low‐dose, chronic exposure or prevalence of endocrine‐disrupting chemicals.

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Section: Discussionmentioning

confidence: 99%

Does bisphenol A bioaccumulate on zebrafish? Determination of tissue bisphenol A level

Eser

Tural

Günal

et al. 2021

Biomedical Chromatography

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“…Aromatase , cyp19 genes ( cyp19a1a and cyp19a1b ) in zebrafish are sensitive biomarkers for assessing the estrogenic potencies of natural estrogens and/or eEDCs ( Jarque et al, 2019 ). In tg ( cyp19a1b:GFP ) zebrafish ( cyp19a1b is a brain specific aromatase gene in zebrafish) GFP expression is under control of 3.4 kb of endogenous cyp19a1b promoter which includes an ERE and possibly also a glia specific enhancer sequence ( Le Page et al, 2008 ; Tong et al, 2009 ).…”

Section: Tg Zebrafish As Nam For Assessing Eedc-related Developmental...mentioning

confidence: 99%

Application of Transgenic Zebrafish Models for Studying the Effects of Estrogenic Endocrine Disrupting Chemicals on Embryonic Brain Development

2022

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Endocrine disrupting chemicals (EDCs) are environmental pollutants that mimic hormones and/or disrupt their function. Estrogenic EDCs (eEDCs) interfere with endogenous estrogen signalling pathway(s) and laboratory animal and human epidemiological studies have provided evidence for a causal link between exposure to them during embryonic/early life and neurological impairments. However, our understanding of the molecular and cellular mechanism(s) underlying eEDCs exposure effects on brain development, tissue architecture and function and behaviour are limited. Transgenic (TG) zebrafish models offer new approach methodologies (NAMs) to help identify the modes of action (MoAs) of EDCs and their associated impacts on tissue development and function. Estrogen biosensor TG zebrafish models have been applied to study eEDC interactions and resulting transcriptional activation (via a fluorescent reporter expression) across the entire body of the developing zebrafish embryo, including in real time. These estrogen biosensor TG zebrafish models are starting to deepen our understanding of the spatiotemporal actions of eEDCs and their resulting impacts on neurological development, brain function and behaviour. In this review, we first investigate the links between early life exposure to eEDCs and neurodevelopmental alterations in model organisms (rodents and zebrafish) and humans. We then present examples of the application of estrogen biosensor and other TG zebrafish models for elucidating the mechanism(s) underlying neurodevelopmental toxicities of eEDCs. In particular we illustrate the utility of combining estrogen biosensor zebrafish models with other TG zebrafish models for understanding the effects of eEDCs on the brain, spanning cellular processes, brain circuitry, neurophysiology and behaviour. Finally, we discuss the future prospects of TG zebrafish models as experimental models for studying more complex scenarios for exposure to contaminant mixtures on neurological development and function.

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“…In addition, observations can be made in real-time allowing for the monitoring of embryogenesis ( Fig. 1) as well as regarding the effects of vaccines in relation to cardiovascular, hepatic, nervous, and endocrine, not to mention, behavioral aspects too [18,40].…”

Section: Zebrafish Model and Vaccines Testing Vaccination Safetymentioning

confidence: 99%

Zebrafish as an alternative animal model in human and animal vaccination research

et al. 2020

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Much of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes.

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Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish

Cited by 24 publications

References 80 publications

Does bisphenol A bioaccumulate on zebrafish? Determination of tissue bisphenol A level

Does bisphenol A bioaccumulate on zebrafish? Determination of tissue bisphenol A level

Application of Transgenic Zebrafish Models for Studying the Effects of Estrogenic Endocrine Disrupting Chemicals on Embryonic Brain Development

Zebrafish as an alternative animal model in human and animal vaccination research

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