Multiplex bacterial growth monitoring in 24‐well microplates using a dual optical sensor for dissolved oxygen and pH

Kocincová, Anna S.; Nagl, Stefan; Arain, Sarina; Krause, Christian; Borisov, Sergey M.; Arnold, Matthias; Wolfbeis, Otto S.

doi:10.1002/bit.21793

Cited by 105 publications

(79 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reference particles were synthesized by incorporating ruthenium(II)tris-(4,7-diphenyl-1,10-phenanthroline) (Ru(dpp) 3 , Sigma-Aldrich) in polyacrylonitrile (PAN) (Sigma-Aldrich) to form Ru(dpp) 3 -PAN particles [25,27]. FITC-AC and Ru(dpp) 3 -PAN (3:1) were mixed with 20 ml of a solution consisting of polyurethane hydrogel (Cardiotech International Inc., Wilmington, Mass., USA) in ethanol/water (90/10 v/v) [25,28]. This mixture was then spread on a transparent poly(vinylidene-chloride) (PVdC) foil (Saran plastic wrap, Dow Chemicals, Midland, Mich., USA).…”

Section: Methodsmentioning

confidence: 99%

Impact of Age and Body Site on Adult Female Skin Surface pH

et al. 2012

View full text Add to dashboard Cite

Background: pH is known as an important parameter in epidermal barrier function and homeostasis. Aim: The impact of age and body site on skin surface pH (pH_SS) of women was evaluated in vivo. Methods: Time domain dual lifetime referencing with luminescent sensor foils was used for pH_SS measurements. pH_SS was measured on the forehead, the temple, and the volar forearm of adult females (n = 97, 52.87 ± 18.58 years, 20–97 years). Every single measurement contained 2,500 pH values due to the luminescence imaging technique used. Results: pH_SS slightly increases with age on all three investigated body sites. There are no significant differences in pH_SS between the three investigated body sites. Conclusion: Adult pH_SS on the forehead, the temple and the volar forearm increases slightly with age. This knowledge is crucial for adapting medical skin care products.

show abstract

Section: Methodsmentioning

confidence: 99%

Impact of Age and Body Site on Adult Female Skin Surface pH

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Borisov et al developed a modified DLR method for the simultaneous detection of CO 2 and O 2 [29]. This method was also used for monitoring of O 2 and pH [30] and pH and temperature [31]. In 2011 Borisov et al developed a DLR setup for the simultaneous detection of the parameters pH, CO 2 , O 2 and temperature in two separated layers which were separated spectrally [32].…”

Section: Et Al Invented a Novel Technique For Luminescence Decay Timmentioning

confidence: 99%

Development of microscopic time‐domain dual lifetime referencing luminescence detection for pH monitoring in microfluidic free‐flow isoelectric focusing

Poehler

Herzog

Suendermann

et al. 2015

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

www.els-journal.com Page 3 Engineering in Life SciencesThis article is protected by copyright. All rights reserved. AbstractA lifetime-based ratiometric microscale pH sensor system and a fluorescence microscopic setup was developed for the in-line observation of pH in free-flow isoelectric focusing based on the principle of time-domain dual lifetime referencing (t-DLR). The t-DLR method has been developed for pH monitoring and various other applications in the fields of environmental monitoring and biotechnology. Here, we introduce the integration of pH sensor microstructures for t-DLR in microfluidic channels and the application of the t-DLR scheme for pH sensing in miniaturized electrophoretic procedures. The pH sensor was inkjet-printed on glass in rows with a length of 10 mm, a height of 404 ± 18 nm and a width of 371 ± 28 µm, and integrated into a microfluidic chip generated by a lasercutting and lamination technique. It had a working range from pH 4 to 8 with a pK A of 6.10 ± 0.01 and fast response times under 500 ms. The sensor was used for the in-line observation of the pH gradient during isoelectric focussing of the proteins β-lactoglobulin A, conalbumin and myoglobin and their identification by their isoelectric point (pI). The obtained pIs were in good agreement with literature data demonstrating the applicability of the pH sensor in microfluidic continuous separations.

show abstract

“…So, pH-sensitive particles can be realized by coupling pH-indicator dyes with the polymer matrix, oxygen-sensitive fluorescence particles are made by coupling of fluorescence dyes with high sensitivity against fluorescence quenching by the triplet oxygen molecule [38,39].…”

Section: Application Of Sensor Particlesmentioning

confidence: 99%

Droplet-Based Microfluidics as a Biomimetic Principle: From PCR-Based Virus Diagnostics to a General Concept for Handling of Biomolecular Information

Köhler

2012

Microdroplet Technology

View full text Add to dashboard Cite

The ability of lithographic fabrication of microchannels for fluid transport on the one hand and the continuously decreasing critical dimensions during the development of microlithography over the last decades generated high expectations on the progress of automated processing of small amounts of substances. In particular, it was expected that large numbers of well distinguished chemical entities-atoms, molecules, nano particles-could become manipulated highly parallel and with high speed. This hope was fed by the imagination of an analogy between the electron transport in integrated circuits and the transport of chemical objects inside microfluidic networks. The experiences of the last both decades had shown that such ideas of a complete analogy are not realistic. The progress of handling of substance-coupled information is much slower as the progress of hard ware development in the electronic devices. But, beside this general disappointment, microfluidics is still promising the arise and growth-up of very important new strategies for organizing chemical systems at the microscale and for supplying functional interfaces between the complex information inside the world of molecules and particles on the one hand and electronic systems on the other hand.Whereas clouds of electrons are manipulated in digital electronic devices, clouds of molecules are transported through microchannels by a convective flow. But, the special conditions in microfluidics cause low Reynolds numbers which reflect the small ratio of channel diameter and volume flow rate to the viscous forces. The microfluidic conditions cause always a laminary structure of flow with steep flow velocity gradients and results in to very high fluidic dispersion of concentration signals if homogeneous fluids are applied. So, the principle high potential of J.M. K€ ohler

show abstract

Multiplex bacterial growth monitoring in 24‐well microplates using a dual optical sensor for dissolved oxygen and pH

Cited by 105 publications

References 30 publications

Impact of Age and Body Site on Adult Female Skin Surface pH

Impact of Age and Body Site on Adult Female Skin Surface pH

Development of microscopic time‐domain dual lifetime referencing luminescence detection for pH monitoring in microfluidic free‐flow isoelectric focusing

Droplet-Based Microfluidics as a Biomimetic Principle: From PCR-Based Virus Diagnostics to a General Concept for Handling of Biomolecular Information

Contact Info

Product

Resources

About