Multiplex Detection of Magnetic Beads Using Offset Field Dependent Frequency Mixing Magnetic Detection

Pourshahidi, Ali Mohammad; Achtsnicht, Stefan; Nambipareechee, Mrinal Murali; Offenhäusser, Andreas; Krause, Hans‐Joachim

doi:10.3390/s21175859

Cited by 9 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For colorization purposes, a reference was taken from the previous works, where the phasor information (amplitude and phase information together) of the MNPs at the higher harmonics was utilized [12,13,[22][23][24][25]. Similarly, in this work, we propose using the modified harmonic amplitude information, A mod = A•cos(ϕ) at the higher harmonics which is directly available from a lock-in-based detection system used for improved sensitivity [2,15,26].…”

Section: Colorization Algorithmmentioning

confidence: 99%

A method for multiplexed and volumetric-based magnetic particle spectroscopy bioassay: mathematical study

Chugh

Liang

Yari

et al. 2023

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

Magnetic particle spectroscopy (MPS) is an emerging biosensing technique that detects target analytes by exploiting the dynamic magnetic responses of magnetic nanoparticles (MNPs). Due to the ease of synthesis and surface chemical functionalization of MNPs, MPS-based bioassays have gained popularity around the globe. One limiting factor for MPS-based assay is the ability to detect multiple analytes simultaneously in a single run, namely, multiplexed bioassay. Several groups have reported the realization of multiplexed bioassays on surface-based MPS platforms by spatially separating reaction areas by using the unique magnetic responses of different MNPs. In this work, we systematically study the magnetization curves (M-H curves) of different types of MNPs and their relationship to the dynamic magnetic responses when subjected to AC magnetic driving fields. Due to the different structures, sizes, and magnetic properties of each kind of MNP, the resulting harmonics are unique. Thus, concurrent quantification (also called “colorization”) of each type of MNP in a mixture is possible by solving the harmonic matrix function. Our results show that the uniqueness of M-H response curves of selected types of MNP and the signal-to-noise ratio (SNR) of the system can affect the accuracy of multiplexed, volumetric-based MPS bioassays. The reported method assumes that each type of MNPs nanoparticles do not interact, and that the magnetic response of the mixture is a linear combination of the responses of each kind of MNP. This assumption may not hold for very dense systems where inter-particle interactions become significant and may require more complex models.

show abstract

Section: Colorization Algorithmmentioning

confidence: 99%

A method for multiplexed and volumetric-based magnetic particle spectroscopy bioassay: mathematical study

Chugh

Liang

Yari

et al. 2023

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

show abstract

“…Among those promising methods, magnetic frequency mixing detection (FMMD) is becoming increasingly popular, with potential to combine analyte-differentiating biosensing techniques [ 9 ], even with MPI imaging modalities simultaneously [ 10 ]. To achieve this, FMMD uses a dual frequency excitation to drive MNPs through saturation and generate a nonlinear magnetic response from the particles [ 11 ], which is characterized by a multi-faceted intermodulation signal that allows for multiplex interpretation and consequently high information gain per measurement [ 12 ]. Due to the small nano-sized tracers and high sensitivity, FMMD is especially suitable for detection of structures on the micro- and nanoscale, as shown by its successful application for detection of SARS [ 13 ] and influenza viruses [ 14 , 15 ], antibodies [ 16 ] and aflatoxin B1 [ 17 ] as well as nanoparticle size differentiation [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Key Contributors to Signal Generation in Frequency Mixing Magnetic Detection (FMMD): An In Silico Study

Engelmann,

Simsek,

Shalaby

et al. 2024

Sensors

Self Cite

View full text Add to dashboard Cite

Frequency mixing magnetic detection (FMMD) is a sensitive and selective technique to detect magnetic nanoparticles (MNPs) serving as probes for binding biological targets. Its principle relies on the nonlinear magnetic relaxation dynamics of a particle ensemble interacting with a dual frequency external magnetic field. In order to increase its sensitivity, lower its limit of detection and overall improve its applicability in biosensing, matching combinations of external field parameters and internal particle properties are being sought to advance FMMD. In this study, we systematically probe the aforementioned interaction with coupled Néel–Brownian dynamic relaxation simulations to examine how key MNP properties as well as applied field parameters affect the frequency mixing signal generation. It is found that the core size of MNPs dominates their nonlinear magnetic response, with the strongest contributions from the largest particles. The drive field amplitude dominates the shape of the field-dependent response, whereas effective anisotropy and hydrodynamic size of the particles only weakly influence the signal generation in FMMD. For tailoring the MNP properties and parameters of the setup towards optimal FMMD signal generation, our findings suggest choosing large particles of core sizes dC>25 nm with narrow size distributions (σ<0.1) to minimize the required drive field amplitude. This allows potential improvements of FMMD as a stand-alone application, as well as advances in magnetic particle imaging, hyperthermia and magnetic immunoassays.

show abstract

“…In the FMMD technique, the MNPs serve as markers to detect a wide range of analytes such as toxins [17], antigens [18], antibodies [19], and viruses [20]. In addition, multiplex detection of different particles was demonstrated, showing the potential of this technique to detect multiple analytes in a single assay [21,22].…”

Section: Introductionmentioning

confidence: 99%

DNA Sensor for the Detection of Brucella spp. Based on Magnetic Nanoparticle Markers

Abuawad,

Ashhab,

Offenhäusser

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Due to the limitations of conventional Brucella detection methods, including safety concerns, long incubation times, and limited specificity, the development of a rapid, selective, and accurate technique for the early detection of Brucella in livestock animals is crucial to prevent the spread of the associated disease. In the present study, we introduce a magnetic nanoparticle marker-based biosensor using frequency mixing magnetic detection for point-of-care testing and quantification of Brucella DNA. Superparamagnetic nanoparticles were used as magnetically measured markers to selectively detect the target DNA hybridized with its complementary capture probes immobilized on a porous polyethylene filter. Experimental conditions like density and length of the probes, hybridization time and temperature, and magnetic binding specificity, sensitivity, and detection limit were investigated and optimized. Our sensor demonstrated a relatively fast detection time of approximately 10 min, with a detection limit of 55 copies (0.09 fM) when tested using DNA amplified from Brucella genetic material. In addition, the detection specificity was examined using gDNA from Brucella and other zoonotic bacteria that may coexist in the same niche, confirming the method’s selectivity for Brucella DNA. Our proposed biosensor has the potential to be used for the early detection of Brucella bacteria in the field and can contribute to disease control measures.

show abstract

Multiplex Detection of Magnetic Beads Using Offset Field Dependent Frequency Mixing Magnetic Detection

Cited by 9 publications

References 43 publications

A method for multiplexed and volumetric-based magnetic particle spectroscopy bioassay: mathematical study

A method for multiplexed and volumetric-based magnetic particle spectroscopy bioassay: mathematical study

Key Contributors to Signal Generation in Frequency Mixing Magnetic Detection (FMMD): An In Silico Study

DNA Sensor for the Detection of Brucella spp. Based on Magnetic Nanoparticle Markers

Contact Info

Product

Resources

About