Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods

Blais, Burton W.; Gaudreault, Melissa; Phillippe, Lucille M.

doi:10.1016/s0956-7135(02)00053-1

Cited by 49 publications

(21 citation statements)

References 10 publications

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“…Further increasing the popularity of these antibody methods are their ability to detect amounts of allergen and gluten consistent with the concentrations discussed as important for safeguarding the allergic or gluten-sensitive consumer [5][6][7][8]. However, the commercial immunodiagnostic methods available for the detection of food allergens and gluten are analyte specific with only a few efforts to develop assays capable of multiple analyses [9,10]. As a result, when testing food for multiple food allergens and gluten, it becomes necessary to perform multiple analytical tests, a timeconsuming and expensive process.…”

Section: Introductionmentioning

confidence: 99%

Multiplex detection of food allergens and gluten

et al. 2015

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To help safeguard the food supply and detect the presence of undeclared food allergens and gluten, most producers and regulatory agencies rely on commercial test kits. Most of these are ELISAs with a few being PCR-based. These methods are very sensitive and analyte specific, requiring different assays to detect each of the different food allergens. Mass spectrometry offers an alternative approach whereby multiple allergens may be detected simultaneously. However, mass spectrometry requires expensive equipment, highly trained analysts, and several years before a quantitative approach can be achieved. Using multianalyte profiling (xMAP®) technology, a commercial multiplex test kit based on the use of established antibodies was developed for the simultaneous detection of up to 14 different food allergens plus gluten. The assay simultaneously detects crustacean seafood, egg, gluten, milk, peanut, soy, and nine tree nuts (almond, Brazil nut, cashew, coconut, hazelnut, macadamia, pine nut, pistachio, and walnut). By simultaneously performing multiple tests (typically two) for each analyte, this magnetic bead-based assay offers built-in confirmatory analyses without the need for additional resources. Twenty-five of the assays were performed on buffer extracted samples, while five were conducted on samples extracted using reduced-denatured conditions. Thus, complete analysis for all 14 allergens and gluten requires only two wells of a 96-well microtiter plate. This makes it possible to include in a single analytical run up to 48 samples. All 30 bead sets in this multiplex assay detected 5 ng/mL of food allergen and gluten with responses greater than background. In addition, 26 of the bead sets displayed signal/noise ratios of five or greater. The bead-based design makes this 30-plex assay expandable to incorporate new antibodies and capture/detector methodologies by ascribing these new detectors to any of the unassigned bead sets that are commercially available.

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Section: Introductionmentioning

confidence: 99%

Multiplex detection of food allergens and gluten

et al. 2015

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“…The major analytical Th methods, including enzyme-linked immunosorbent assay (ELISA), mass spectrometry (MS) and polymerase chain reaction (PCR) are discussed in detail in this chapter. While the majority of commercially available allergen detection methods are single allergen assays, multi-allergen detection methods have recently been developed using a multiplex enzyme immunoassay [7][8][9][10], MS [11][12][13][14][15][16] or DNA amplifi cation [17,18]. Understanding fi the limitations of available methods for food allergen quantitation, specifi-fi cally with respect to sample extraction, thermal processing, and biomarker selection, will improve method selection, establish appropriate allergen control plans, and ultimately protect allergic consumers.…”

Section: ) Th E Food Allergen Labeling and Consumer Protectionmentioning

confidence: 99%

Detection of Allergen Markers in Food: Analytical Methods

et al. 2016

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“…The detection limit is around 1 mg/kg, however, detectability is affected by the food matrix and the processing history of the food (Poms et al, 2003). PCR methods are also available There are also systems for multiple nut allergen detection (Ben Rejeb et al, 2003;Blais et al, 2003;Pomés et al, 2003).…”

Section: Peanutsmentioning

confidence: 99%

Opinion of the Scientific Panel on Dietetic products, nutrition and allergies [NDA] on a request from the Commission relating to the evaluation of allergenic foods for labelling purposes

2004

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Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods

Cited by 49 publications

References 10 publications

Multiplex detection of food allergens and gluten

Multiplex detection of food allergens and gluten

Detection of Allergen Markers in Food: Analytical Methods

Opinion of the Scientific Panel on Dietetic products, nutrition and allergies [NDA] on a request from the Commission relating to the evaluation of allergenic foods for labelling purposes

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