Multiplex immunoassays using surface modification-mediated porous layer open tubular capillary

A Highly Sensitive Capillary-Based Immunosensor by Combining with Peroxidase Nanocomplex-Mediated Signal Amplification for Detection of Procalcitonin in Human Serum

“…8,13 While PCT detection is performed in the present study, the proposed BS-capillary-based immunosensor can be applied for detection of different biomarkers, since the antibody immobilization is achieved using well-known silica chemistry and the biotin−streptavidin signal amplification system is compatible with the immunoassays. We also expect that the introduction of the biotin−streptavidin signal amplification system proposed in the study could be utilized for multiplex detection of biomarkers, as we have recently reported, 14 which would stimulate further studies on the immunoassays based on capillary sensors.…”

“…8,11 In addition, the capillary wall possesses an excellent light-wave-guiding property; thus, capillary-based immunosensors are especially suitable for sensitive optical detection such as chemiluminescence (CL) or laser-induced fluorescence. 8,11,14,17 Moreover, capillary-based immunosensors feature the advantage of easy integration and automatization, which meet the requirements for miniaturized bioanalysis nowadays.…”

“…With either sophisticated design and manipulation or complicated surface modification, the detection sensitivity of these sensors can be improved by 3-to 10-fold compared to that of a normal capillary-based sensor. 13,14,18 A promising strategy to further enhance the detection sensitivity of microcapillary-based sensors is to combine them with an effective signal amplification system, which has been adopted to improve their analytical performance in various fields. 19−27 Surprisingly, to the best of our knowledge, up to now, there is no report about the application of a signal amplification system in capillary-based immunoassays.…”

“…Combining high specificity of immunological reaction with sensitivity and convenient operation, immunosensors or miniaturized immunoassay devices have emerged as important assays for quantitative detection of biomarkers in clinical diagnosis. − Among various kinds of immunosensors, capillary-based immunosensors, which employ fused silica microcapillary tubes as support for bioreagent immobilization, have been developed rapidly in recent years. ,− Compared to other solid-material-based sensors, capillary-based immunosensors show several essential merits for immunoassays. It is well known that assays based on microcapillaries offer low sample/reagent consumption, fast mass-transfer rate, and short analysis time. , In addition, the capillary wall possesses an excellent light-wave-guiding property; thus, capillary-based immunosensors are especially suitable for sensitive optical detection such as chemiluminescence (CL) or laser-induced fluorescence. ,,, Moreover, capillary-based immunosensors feature the advantage of easy integration and automatization, which meet the requirements for miniaturized bioanalysis nowadays.…”

Chemiluminescent Optical Fiber Immunosensor Combining Surface Modification and Signal Amplification for Ultrasensitive Determination of Hepatitis B Antigen

“…Then it was washed successively with 0.1 M NaOH for 1 h and water for 15 min. After drying under N 2 , a porous layer was fabricated on the fiber surface through an in situ two-phase reaction, the procedure of which was similar as that reported in our previous study [ 36 ]. Briefly, the pre-treated fiber was inserted in the reaction solution containing 0.05 g CTAB, 0.03 g urea, 1.5 mL H 2 O, 46 μL n-amyl alcohol, 1.5 mL cyclohexane, and 50 μL TEOS.…”