Multiplex liquid chromatography-tandem mass spectrometry for the detection of wheat, oat, barley and rye prolamins towards the assessment of gluten-free product safety

Manfredi, Anita; Mattarozzi, Monica; Giannetto, Marco; Careri, Maria

doi:10.1016/j.aca.2015.09.008

Cited by 49 publications

(38 citation statements)

References 39 publications

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“…However, this is a challenging task due to polymorphism of gluten proteins, which vary from sample to sample [7,46,48]. When comparing the use of modern techniques such as LC-MS and ELISA, previous studies show no correlation between ELISA results and the relative content of peptides determined by MS [48].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 98%

“…A progressive number of approaches using MS have been developed, offering great potential in this area [9,37,46,52].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…In the last years, MS techniques have overcome some limitations associated to antibody-based methods, such as cross-reactivity and discriminating capacity of gluten protein sources in a single run [46]. Recently, label-free MS experiments have been improved in order to quantify specifically CD epitopes [43].…”

Section: Proteomic Tools For Gluten Detectionmentioning

confidence: 99%

“…In this context, methods based on MS have been alternatively proposed for gluten quantification, since it can detect specific and comprehensive peptides with good sensitivity and precision, due to the high-throughput data analysis capacity [10,46]. A progressive number of approaches using MS have been developed, offering great potential in this area [9,37,46,52].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…However, measurements by commercial ELISA kits are inconsistent and require standardization of results due to the lack of certified reference material and the diversity of kits using different test conditions [7,46].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

See 4 more Smart Citations

Determination of Gluten Peptides Associated with Celiac Disease by Mass Spectrometry

Alves¹,

D’Almeida²,

Ferreira³

2017

Celiac Disease and Non-Celiac Gluten Sensitivity

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Gluten is a big protein network composed of monomeric fraction (prolamins) and polymeric fraction (glutelins), occurring in many cereal-based products, especially in those containing wheat. Gluten peptides can trigger food allergies and intolerances, including inflammatory reactions as the celiac disease, an autoimmune disorder of the small intestine characterized by mucosal degeneration and villous atrophy. The treatment is the permanent exclusion of gluten from diet. However, gluten analysis is a very difficult task, due to the high complexity of polypeptides and the lack of consensus on the most appropriate analytical method. Proteomics approaches, combining liquid chromatography and mass spectrometry in tandem (LC-MS/MS), have been pointed as the most promising nonimmunological techniques for gluten detection. LC-MS analyses associated with bioinformatics and specific-prolamin database can solve methodological limitations since it is based on the accurate molecular mass of peptide biomarkers. One of the major contributions of proteomics has been the identification of epitopes of gluten peptides responsible for wheat-related diseases. Recent works have defined grain-specific gluten peptides and also the lowest concentration at which peptides could be confidently detected. Proteomic application for gluten quantification should support not only regulatory limits in processed foods, but also the safety of consumers about food labeled as gluten-free.

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Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 98%

“…A progressive number of approaches using MS have been developed, offering great potential in this area [9,37,46,52].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Section: Proteomic Tools For Gluten Detectionmentioning

confidence: 99%

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

See 3 more Smart Citations

Determination of Gluten Peptides Associated with Celiac Disease by Mass Spectrometry

Alves¹,

D’Almeida²,

Ferreira³

2017

Celiac Disease and Non-Celiac Gluten Sensitivity

View full text Add to dashboard Cite

show abstract

Advances in Food Allergen Analysis

et al. 2016

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Recent advances in biosensors for diagnosis of celiac disease: A review

Gupta

Kaushal

Kumar

et al. 2018

Biotech & Bioengineering

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Celiac disease (CD) is an intestinal issue activated by the inappropriate immune reaction towards gluten protein of wheat, rye, barley, oats, and autoantigen, tissue transglutaminase. Regardless of the accessibility of immunochemical conventions for research facility analysis of CD, there is as yet a need of speedier, less expensive, and simpler devices for diagnosing CD. This review concentrates on progresses in biosensors for diagnosing CD in perspective of the scaled down hardware, multianalyte discovery and low sample volume necessity. Various recently developed biosensors in this field are presented.

show abstract

Multiplex liquid chromatography-tandem mass spectrometry for the detection of wheat, oat, barley and rye prolamins towards the assessment of gluten-free product safety

Cited by 49 publications

References 39 publications

Determination of Gluten Peptides Associated with Celiac Disease by Mass Spectrometry

Determination of Gluten Peptides Associated with Celiac Disease by Mass Spectrometry

Advances in Food Allergen Analysis

Recent advances in biosensors for diagnosis of celiac disease: A review

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