Multiplex-PCR and PCR-RFLP assays to monitor water quality against pathogenic bacteria

Abd‐El‐Haleem, Desouky; Kheiralla, Zeinab H.; Zaki, Sahar; Rushdy, Abeer Ahmed; Abd-El-Rahiem, Walaa

doi:10.1039/b307476e

Cited by 24 publications

(16 citation statements)

References 32 publications

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“…MPCR methods for detecting several genes of these bacteria have been reported previously for E. coli O157:H7 (9,10,13,16,(21)(22)(23)(24), Salmonella spp (13,21,(25)(26)(27), and V. cholerae (21,28,29 ). However, none of the reported MPCR methods simultaneously detected all three viable pathogens present in the same sample.…”

mentioning

confidence: 99%

Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi

Morin

Gong

2004

Clinical Chemistry

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confidence: 99%

Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi

Morin

Gong

2004

Clinical Chemistry

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“…Culture-based detection can be time consuming and does not account for viable but nonculturable strains (Iijima et al, 2007;Khan et al, 2007;Ram et al, 2008). Molecular methods including PCR are faster, can detect all viable bacteria in a sample, and can also detect pathogens in very low concentrations (Abd-El-Haleem et al, 2003). However, one of the major disadvantages of PCR as it relates to environmental samples is that there is a window of opportunity where it can detect non-viable bacteria (i.e.…”

Section: Microbial Contamination In Private Systemsmentioning

confidence: 99%

Associations between fecal indicator bacteria prevalence and demographic data in private water supplies in Virginia

Smith

Krometis

Hagedorn

et al. 2014

Journal of Water and Health

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Over 1.7 million Virginians rely on private water systems to supply household water. The heaviest reliance on these systems occurs in rural areas, which are often underserved in terms of financial resources and access to environmental health education. As the Safe Drinking Water Act (SDWA) does not regulate private water systems, it is the sole responsibility of the homeowner to maintain and monitor these systems.Previous limited studies indicate that microbial contamination of drinking water from private wells and springs is far from uncommon, ranging from 10% to 68%, depending on type of organism and geological region. With the exception of one thirtyyear old government study on rural water supplies, there have been no documented investigations of links between private system water contamination and household demographic characteristics, making the design of effective public health interventions, very difficult.The goal of the present study is to identify potential associations between concentrations of fecal indicator bacteria (e.g. coliforms, E. coli) in 831 samples collected at the point-of-use in homes with private water supply systems and homeowner-provided demographic data (e.g. homeowner age, household income, education, water quality perception). Household income and the education of the perceived head of household were determined to have an association with bacteria concentrations. However, when a model was developed to evaluate strong associations between total coliform presence and potential predictors, no demographic parameters were deemed significant enough to be included in the final model. Of the 831 samples tested, 349 (42%) of samples tested positive for total coliform and 55 (6.6%) tested positive for E. coli contamination. Chemical and microbial source tracking efforts using fluorometry and qPCR suggested possible E. coli contamination from human septage in 21 cases. The findings of this research can ultimately aid in determining effective strategies for public health intervention and gain a better understanding of interactions between demographic data and private system water quality.iii ACKNOWLEDGMENTS

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“…2). Previously, Abd-El- Haleem et al (2003), reported that HinfI is the most suitable enzyme for RFLP analysis for the 16S rDNA amplicons.…”

Section: Pcr-rflp Fingerprints Of the 16s Rdna Genementioning

confidence: 99%

Isolation and molecular characterization of some copper biosorped strains

Zaki

Farag

2010

Int. J. Environ. Sci. Technol.

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ABSTRACT:The biosorption of copper (II) from aqueous solution using different bacterial strains was studied.Copper-biosorbing bacteria were isolated from tannery effluent collected from Borg Al-Arab, Alexandria, Egypt. These isolates displayed different degrees of copper biosorption under aerobic conditions. Based on 16S rDNA gene sequence analysis, three of them (S2, S5 and S7) were identified as Chryseobacterium sp., Enterobacter sp. and Stenotrophomonas sp., respectively. Initial copper (II) ion concentrations from 25-250 mg/L at constant temperature 30 ºC were studied. The residual copper (II) concentration and its toxicity effect in solution were determined using atomic absorption spectrophotometer and bioluminescent bioreporter. The bioluminescence inhibition of strain (S5) reached to 91.4 % as compared with the strain (S7) reached to 83.3 % at 225 mg/L of copper ion where the maximum biosorption efficiency for S5 and S7 were 71 % and 70.1 % correspondingly using atomic absorption. The bioluminescent bioreporter was proved to be fast and accurate technique for measurement the toxicity effect of residual copper (II) in solution.

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Multiplex-PCR and PCR-RFLP assays to monitor water quality against pathogenic bacteria

Cited by 24 publications

References 32 publications

Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi

Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi

Associations between fecal indicator bacteria prevalence and demographic data in private water supplies in Virginia

Isolation and molecular characterization of some copper biosorped strains

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